Identification and pathogenicity of bacteria from Gryllotalpa gryllotalpa L. (Orthoptera: Gryllotalpidae)

Yıl 2013, Cilt: 4 Sayı: 2, 89 - 108, 13.07.2016

Kazim Sezen Serife Iscı Hacer Muratoglu Kadriye Inan

Öz

Gryllotalpa gryllotalpa (Orthoptera: Gryllotalpidae)’nın bakteriyel mikrobiyotası, araştırılmıştır ve G. gryllotalpa’nın nimflerinden 10 farklı bakteriyel aerobik tür izole edilmiştir. G. gryllotalpa en önemli tarım zararlılarından biridir. Bu çalışmada, bakterilerin morfolojik, fizyolojik ve biyokimyasal özelliklerini ve API 20E ve API 50CH panel test sistemleri sayesinde metabolik enzim profillerini belirledik. Ayrıca, izolatların moleküler seviyede belirlenmesi için 16S rRNA geni dizi analizi ve Hiper değişken (HV) analizleri uygulanmıştır. Bakteriyel türler, Bacillus weihenstephanensis (Gg1), Serratia ureilytica (Gg2), Bacillus simplex (Gg3), Bacillus nanhaiensis (Gg5), Bacillus sp. (Gg6), Bacillus gibsonii (Gg7), Citrobacter braakii (Gg8), Paenibacillus sp. (Gg9), Enterobacter sp. (Gg10), and Providencia rettgeri (Gg11) olarak belirlenmiştir. Bütün bakteriyel türler, on gün boyunca 3.6×109 hücre/ml dozda G. gryllotalpa’nın nimflerine karşı test edilmiştir. Bu sonuçlar, özellikle B. weihenstephanensis (Gg1) ve Bacillus nanhaiensis (Gg5) bakterilerinin, G. gryllotalpa’nın mikrobiyal mücadelesinde değerli bir etken olabileceğini göstermiştir.

Anahtar Kelimeler

Bakteriyel mikrobiyota, biyolojik mücadele, HV analizi, Gryllotalpa gryllotalpa, böcek patolojisi, 16S rRNA geni

Gryllotalpa gryllotalpa L. (Orthoptera: Gryllotalpidae)’den izole edilen bakterilerin tanımlanması ve patojenitesi

Öz

The bacterial microbiota of the Gryllotalpa gryllotalpa (Orthoptera: Gryllotalpidae) was investigated and 10 different aerobic bacterial strains were isolated from the nymphs of G. gryllotalpa. It is one of the most important agricultural pests. We determined the morphological, physiological, and biochemical properties of the bacteria and metabolic enzyme profiles by API 20E and API 50CH panel test systems. Additionally 16S rRNA gene sequence and Hyper variable (HV) analyses were performed to determine the strains at the molecular level. They were identified as Bacillus weihenstephanensis (Gg1), Serratia ureilytica (Gg2), Bacillus simplex (Gg3), Bacillus nanhaiensis (Gg5), Bacillus sp. (Gg6), Bacillus gibsonii (Gg7), Citrobacter braakii (Gg8), Paenibacillus sp. (Gg9), Enterobacter sp. (Gg10), and Providencia rettgeri (Gg11), according to results. All these bacterial strains were tested against the nymphs of G. gryllotalpa at 3.6×109 cfu/ml dose, within ten days. Our results indicate that especially B. weihenstephanensis (Gg1) and Bacillus nanhaiensis (Gg5) may be valuable as a microbial control agent for G. gryllotalpa.

Anahtar Kelimeler

Bacterial microbiota, biological control, HV analyses, Gryllotalpa gryllotalpa, insect pathology, 16S rRNA gene.

Kaynakça

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