Evaluation of the Biological Activities of Royal Jelly on Prostate and Breast Cancer Cells

Öz

Due to their high toxicity to healthy body cells, chemotherapy drugs used to treat cancer pose a serious threat to the organism. Recent studies have encouraged the utilization of bee products to prevent and treat the cancer. The specific food for the queen honeybee larva (Apis mellifera), royal jelly (RJ) is a yellow, milky, cremy product of the bee which is stowed from the mandibular and hypopharyngeal glands of the worker honeybee. According to the reports, RJ may have cytotoxic effects in animal models. However, RJ's cytotoxic and antioxidant properties on PC-3 prostate cancer and MCF-7 breast cancer cell lines have yet to be thoroughly investigated at the cellular level. Therefore, the antioxidant and anti-proliferative activities of RJ in PC-3 prostate cancer cells and MCF-7 breast cancer cells were evaluated to investigate a chemopreventive strategy to increase cancer therapy efficacy. WST-1 assay was used to measure cell proliferation, and levels of reactive oxygen species (ROS) and lipid peroxidation (LPO) were analyzed to look at antioxidant activities. According to the data of this research, RJ act as prooxidants in PC-3 and MCF-7 cancer cell lines by lessening cellular viability and raising ROS and LPO formation.

Anahtar Kelimeler

• Royal jelly
• WST-1 Assay
• Prostate cancer
• Breast cancer
• Lipid peroxidation

Kaynakça

1. [1] Filipič B, Gradišnik L, Rihar K, Šooš E, Pereyra A, Potokar J (2015). The influence of royal jelly and human interferon-alpha (HuIFN-αN3) on proliferation, glutathione level and lipid peroxidation in human colorectal adenocarcinoma cells in vitro. Arhiv Za Higijenu Rada i Toksikologiju, 66 (4): 269-274. doi: 10.1515/aiht-2015-66-2632
2. [2] Ramanathan ANKG, Nair AJ, Sugunan VS (2018). A review on Royal Jelly proteins and peptides. Journal of Functional Foods 44: 255-264. doi: 10.1016/j.jff.2018.03.008
3. [3] Dobritzsch D, Aumer D, Fuszard M, Erler S, Buttstedt A (2019). The rise and fall of major royal jelly proteins during a honeybee (Apis mellifera) workers’ life. Ecology and Evolution 9 (15): 8771-8782. doi: 10.1002/ece3.5429
4. [4] Coutinho D, Karibasappa SN, Mehta DS (2018). Royal jelly antimicrobial activity against periodontopathic bacteria. Journal of Interdisciplinary Dentistry 8 (1): 18. doi: 10.4103/ jid.jid_72_17 Darendelioglu, E (2020). Neuroprotective effects of chrysin on diclofenac-induced apoptosis in SH-SY5Y cells. Neurochemical Research 45 (5): 1064-1071. doi: 10.1007/s11064-020-02982-8.
5. [5] Park HG, Kim BY, Park MJ, Deng Y, Choi YS et al. (2019). Antibacterial activity of major royal jelly proteins of the honeybee (Apis mellifera) royal jelly. Journal of Asia-Pacific Entomology 22 (3): 737-741. doi: 10.1016/j.aspen.2019.06.005
6. [6] Ahmad S, Campos MG, Fratini F. Altaye SZ, Li J (2020). New insights into the biological and pharmaceutical properties of royal jelly. International Journal of Molecular Sciences 21 (2): 382- 407. doi: 10.3390/ijms21020382
7. [7] Park MJ, Kim BY, Deng Y, Park HG, Choi YS et al. (2020). Antioxidant capacity of major royal jelly proteins of honeybee (Apis mellifera) royal jelly. Journal of Asia-Pacific Entomology, 23 (2): 445-448. doi: 10.1016/j.aspen.2020.03.007
8. [8] Guo J, Wang Z, Chen Y, Cao J, Tian W et al. (2021). Active components and biological functions of royal jelly. Journal of Functional Foods 82: 104514. doi: 10.1016/j.jff.2021.104514

9. [9] Lercker G, Capella P, Conte LS, Ruini F, Giordani G (1981). Components of royal jelly: I. Identification of the organic acids. Lipids 16 (12): 912-919. doi:10.1007/BF02534997
10. [10] Viuda‐Martos M, Ruiz‐Navajas Y, Fernández‐López J, Pérez‐Álvarez JA (2008). Functional properties of honey, propolis, and royal jelly. Journal of Food Science 73 (9): 117-124. doi:10.1111/ j.1750-3841.2008.00966.x
11. [11] Pasupuleti VR, Sammugam L, Ramesh N, Gan SH (2017). Honey, propolis, and royal jelly: a comprehensive review of their biological actions and health benefits. Oxidative Medicine and Cellular Longevity 2017. doi: 10.1155/2017/1259510
12. [12] Kocot J, Kiełczykowska M, Luchowska-Kocot D, Kurzepa J, Musik I (2018). Antioxidant potential of propolis, bee pollen, and royal jelly: possible medical application. Oxidative Medicine and Cellular Longevity 2018: 1-29. doi: 10.1155/2018/7074209
13. [13] Liu HL, Jiang WB, Xie MX (2010). Flavonoids: recent advances as anticancer drugs. Recent Patents on Anti-cancer Drug Discovery 5 (2): 152-164. doi: 10.2174/157489210790936261
14. [ 14] Zhang HW, Hu JJ, Fu RQ, Liu X, Zhang YH et al. (2018). Flavonoids inhibit cell proliferation and induce apoptosis and autophagy through downregulation of PI3Kγ mediated PI3K/AKT/ mTOR/p70S6K/ULK signaling pathway in human breast cancer cells. Scientific Reports 8 (1): 11255. doi: 10.1038/ s41598-018-29308-7
15. [15] Hazafa A, Rehman, KU, Jahan N, Jabeen Z (2019). The role of polyphenol (flavonoids) compounds in the treatment of cancer cells. Nutrition and Cancer 72 (3): 386-397. doi:10.1080/0163 5581.2019.1637006
16. [16] Premratanachai P, Chanchao C (2014). Review of the anticancer activities of bee products. Asian Pacific Journal of Tropical Biomedicine 4 (5): 337-344. doi: 10.12980/APJTB.4.2014C1262
17. [17] Badolato M, Carullo G, Cione E, Aiello F, Caroleo MC (2017). From the hive: Honey, a novel weapon against cancer. European Journal of Medicinal Chemistry 142: 290-299. doi: 10.1016/j. ejmech.2017.07.064
18. [18] Farooqi AA, Romero MA, Aras A, Qureshi MZ, Wakim LH (2019). Honey and popolis‐mediated regulation of protein networks in cancer cells. Nutraceuticals and Natural Product Derivatives: Disease Prevention & Drug Discovery 137-144. doi: 10.1002/9781119436713.ch6
19. [19] Aykutoglu G, Tartik M, Darendelioglu E, Ayna A, Baydas G (2020). Melatonin and vitamin E alleviate homocysteine‐induced oxidative injury and apoptosis in endothelial cells. Molecular Biology Reports 47 (7): 5285-5293.
20. [20] Özbolat SN, Ayna A (2021). Chrysin suppresses HT-29 cell death induced by diclofenac through apoptosis and oxidative damage. Nutrition and Cancer 73 (8): 1419-1428. doi:10.1080/ 01635581.2020.1801775
21. [21] Fontana R, Mendes MA, De Souza BM, Konno K, César LMM et al. (2004). Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera). Peptides 25 (6): 919- 928. doi: 10.1016/j.peptides.2004.03.016
22. [22] Bengü AŞ, Ayna A, Özbolat SN, Tunç A, Aykutoğlu G et al. (2020). Content and antimicrobial activities of Bingol Royal Jelly. Türk Tarım ve Doğa Bilimleri Dergisi 7 (2): 480-486. doi:10.30910/ turkjans.725977
23. [23] Yang YC, Chou WM, Widowati DA, Lin IP, Peng CC (2018). 10-hydroxy-2-decenoic acid of royal jelly exhibits bactericide and anti-inflammatory activity in human colon cancer cells. BMC Complementary and Alternative Medicine 18 (1): 202. doi: 10.1186/s12906-018-2267-9
24. [24] Miyata Y, Sakai H (2018). Anti-cancer and protective effects of royal jelly for therapy-induced toxicities in malignancies. International Journal of Molecular Sciences 19 (10): 3270- 3285. doi: 10.3390/ijms19103270
25. [25] Al-Kushi AG, Header EA, ElSawy NA, Moustafa RA, Alfky NAA (2018). Antioxidant effect of royal jelly on immune status of hyperglycemic rats. Pharmacognosy Magazine 14 (58): 528. doi:10.4103/pm.pm_87_18
26. [26] Takahama H, Shimazu T (2006). Food‐induced anaphylaxis caused by ingestion of royal jelly. The Journal of Dermatology 33 (6): 424-426. doi: 10.1111/j.1346-8138.2006.00100.x
27. [27] Kimura Y (2008). Antitumor and antimetastatic actions of various natural products. In Studies in Natural Products Chemistry 34: 35-76. doi: 10.1016/S1572-5995(08)80024-5
28. [28] Turan I, Demir S, Misir S, Kilinc K, Mentese A et al. (2015). Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines. Tropical Journal of Pharmaceutical Research 14 (5): 777-782. doi:10.4314/tjpr.v14i5.5
29. [29] Taniguchi Y, Kohno K, Inoue SI, Koya-Miyata S, Okamoto I et al. (2003). Oral administration of royal jelly inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice. International Immunopharmacology 3 (9): 1313-1324. doi: 10.1016/s1567-5769(03)00132-2
30. [30] Bincoletto C, Eberlin S, Figueiredo CA, Luengo MB, Queiroz ML (2005). Effects produced by Royal Jelly on haematopoiesis: relation with host resistance against Ehrlich ascites tumour challenge. International Immunopharmacology 5 (4): 679-688. doi: 10.1016/j.intimp.2004.11.015
31. [31] Paulsen CE, Carroll KS (2013). Cysteine-mediated redox signaling: chemistry, biology, and tools for discovery. Chemical Reviews 113 (7): 4633-4679. doi: 10.1021/cr300163e
32. [32] Klaunig JE, Xu Y, Isenberg JS, Bachowski S, Kolaja KL et al. (1998). The role of oxidative stress in chemical carcinogenesis. Environmental Health Perspectives 106 (suppl 1): 289-295. doi:10.1289/ehp.98106s1289
33. [33] Jafari M, Rezaei M, Kalantari H, Hashemitabar M (2013). Determination of cell death induced by lovastatin on human colon cell line HT29 using the comet assay. Jundishapur Journal of Natural Pharmaceutical Products 8 (4): 187-191. doi: 10.17795/jjnpp-10951