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Bazı Fumaria L. (Papaveraceae) Türlerinin Metanolik Ekstraktlarının Kanser Hücre Hatları Üzerindeki İn Vitro Sitotoksik Etkileri

Yıl 2024, , 25 - 29, 28.06.2024
https://doi.org/10.46810/tdfd.1287018

Öz

Fumaria cinsi (Papaveraceae) halk arasında “Şahtere” olarak bilinir. Son derece zengin bileşiklere sahip olduğu kanıtlanmış olan cins, halk arasında birçok rahatsızlığın tedavisinde kullanılmaktadır. Bu çalışmada, Fumaria asepala Boiss. ve Fumaria schleicheri Soy.-Will. subsp. microcarpa Boiss. ex Hausskn. taksonlarının toprak üstü kısımlarının metanolik ekstraktının Beas-2B, sh-sy5, HCT116 ve A549 hücre hatlarına karşı sitotoksik özellikleri araştırıldı. Kanser hücrelerinin canlılığındaki değişiklikler 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) yöntemi kullanılarak tespit edildi. Sonuçlar, F. asepala ve F. schichleiri subsp. microcarpa’nın metanolik ekstraktının özellikle kolon (HCT-116) kanseri hücrelerinin hücre canlılığında istatistiksel olarak anlamlı bir azalma olduğunu gösterirken, akciğer (A549) kanseri ve beyin (sh-sy5) kanseri hücreleri üzerinde sitotoksik etkinlik sağlamamıştır. Ayrıca bitkisel ekstraktlar, sağlıklı akciğer hücreleri (Beas-2B) üzerinde ise herhangi bir toksik etki yaratmamıştır.

Kaynakça

  • Aksoy C, Yücecan S, Çiftçi N, Tayfur M, Akgün B, Taşcı N. Local plants used for treatment in cancer disease. J. of Nutr. and Dietetics. 1988;17(1): 111-120.
  • Yılmaz B. Investigation of Antitumoral Activity of Plant Extracts Collected from Various Regions [Master Thesis]. Tekirdağ: Namık Kemal University, Institute of Health Science; 2018.
  • Çoban T, Çitoǧlu G, Sever B, İşcan M. Antioxidant activities of plants used in traditional medicine in Türkiye. Pharmaceutical Biology. 2003;41(8):608-613.
  • Goncalves S, Romano A. The medicinal potential of plants from genus Plantago (Plantaginaceae). Industrial Crops and Products. 2016;83(1):213-226.
  • Baydar H. Tıbbi, Aromatik ve Keyf Bitkileri Bilimi ve Teknolojisi. 1st ed. Türkiye: Suleyman Demirel University Publications; 2005;111-115.
  • Craker LE, Gardner Z, Etter SC. Herbs in American Fields: A Horticultural Perspective of Herband Medical Plant Production in the United Sates. Horticultural Science. 2003;38:977–983.
  • url-1: http://www.turktarim.gov.tr/Haber/64/turkiye-tibbi-ve-aromatik-bitki-yetistiriciliginde-oncu-ulkelerden).
  • Pérez-Gutiérrez MA, Romero-García AT, Salinas MJ, Blanca G, Fernández C, Suárez-Santiago VN. Phylogeny of the tribe Fumarieae (Papaveraceae) based on chloroplast and nuclear DNA sequences: Evolutionary and biogeographic implications. American J. of Bot. 2012;99:517–528.
  • Tutin TG, Burges NA, Chater AO, Edmonson JR, Heywood VH, Moore DM, et al. Psilotaceae to Platanaceae. In Flora Europaea, 2nd ed.; Cambridge, UK: Cambridge University Press; 2010;(1):306–311.
  • Orhan IE, Sener B, Musharraf SG. Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Exp. Toxicol. Pathol. 2012;64:205–209.
  • Sousek J, Guedon D. Alkaloids and organic acids content of Eight Fumaria species. Phytochem Anal. 1999;10:6-11.
  • Paltinean R, Mocan A, Vlase L, Gheldiu AM, Cri¸san G, Ielciu I, et al. Evaluation of Polyphenolic Content, Antioxidant and Diuretic Activities of Six Fumaria Species. Molecules. 2017;22:639-650.
  • Dutta R, Sharma MK, Jha MA. Review on Ethnobotanical, Phytochemistry, Bioactivities and Medicinal Mysteries of Fumaria officinalis (Common Fumitory). EAS J. Pharm. Pharmacol. 2019;1:99–105.
  • Al-Snafi AE. Constituents and Pharmacology of Fumaria officinalis-A Review. IOSR J. Pharm. 2020;10:17–25.
  • Ivanov IG, Vrancheva RZ, Marchev AS, Petkova NT, Aneva IY, Panteley DP, Georgiev VG, Pavlov AI. Antioxidant Activities and Phenolic Compounds in Bulgarian Fumaria Species. Int. J. Curr. Microbiol. Appl. Sci. 2014;3:296–306.
  • Zhang R, Guo Q, Kennelly EJ, Long C, Chai X. Diverse Alkaloids and Biological Activities of Fumaria (Papaveraceae): An Ethnomedicinal Group. Fitoterapia. 2020;146: 104697.
  • Paltinean R, Toiu A, Wauters JN, Frédérich M, Tits M, Angenot L, et al. Identification and Determination of Alkaloids in Fumaria Species from Romania. Dig. J. Nanomater. Biostruct. 2013;8:817–824.
  • Păltinean R, Ielciu I, Hanganu D, Niculae M, Pall E, Angenot L, et al. Biological Activities of Some Isoquinoline Alkaloids from Fumaria schleicheri Soy. Will. Plants. 2022;11(9):1202.
  • Vrancheva R, Ivanov I, Marchev A, Pavlov A. Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method. J. Biosci. Biotechnol. 2012;1:255–259.
  • Çelik AK, Yılmaz Sancar P, Icen Taskın I, Kürşat M. Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines. Artvin Coruh University Journal of Forestry Faculty. 2024;25(1): 104-110.
  • Yener I, Olmez OT, Ertaş A, Yılmaz Ma, Fırat M, Kandemir SI, Öztürk M. A detailed study on chemical and biological profile of nine Euphorbia species from Türkiye with chemometric approach: Remarkable cytotoxicity of E. fistulasa and promising tannic acid content of E. eriophora. Indust. Crops and Prod. 2018;123:442-453.
  • Çelik TA, Aslantürk ÖS, Yılmaz EŞ, Güzel Y. Antioxidant and Cytotoxic Activities of Fumaria parviflora Lam. and Fumaria capreolata L. KSU J. Agric Nat. 2022;25(4):819-827.
  • Tabrizi FH, Irian S, Amanzadeh A, Heidarnejad F, Gudarzi H, Salimi M. Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines. Res Pharm Sci. 2016;11(2):152-9.
  • Seyed RM, Nassiri-Asl M, Farahani-Nick Z, Savad S, Seyed KF. Protective effects of Fumaria vaillantii extract on carbon tetrachloride induced hepatotoxicity in rats. Pharmacologyonline. 2007;3:385-393.
  • Rathi A, Srivastava A, Shirwaikar A, Rawat A. Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine. Phytomedicine. 2008;15:470-477.
  • Hussain T, Hefazat HS, Sheeba F, Sweety K, Vijayakumar M, Rao CV. Chemopreventive effect of Fumaria indica that modulates the oxidant antioxidant inbalance during N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Wistar rats. Asian Pacific J. of Tropical Biomed. 2012;2:995-1001.
  • Ezzat M, Kian A, Bagher M, Seyedeh ZOS. Protective activity of Fumaria vaillantii Loisel and mono methyl fumrate on acetoaminophen induced hepatotoxicity in mice. Int J Pharmacology. 2012;8:177-184.
  • Aveen N, Adham AM, Naqishbandi TE. Cytotoxicity and apoptosis induction by Fumaria officinalis extracts in leukemia and multiple myeloma cell lines. J. of Ethnopharmacology. 2021;266:113458.
  • Sattari R, Khayati GR, Hoshyar R. Biosynthesis and characterization of silver nanoparticles capped by biomolecules by fumaria parviflora extract as green approach and evaluation of their cytotoxicity against human breast cancer MDA-MB-468 cell lines. Materials Chem. and Physics. 2020;241:122438.
  • Aljanaby A. Cytotoxicity of Fumaria officinalis on Leishmania tropica, L20B cell lines and bacteria in vitro. J. of Global Pharma Technology. 2019;11:171-175.
  • Sadaoui BK, Gontier E, Telliez MS, Lequart-Pillon M, Ouadid-Ahidouch H, Maiza F. Characterization of isoquinolin alkaloids from Fumaria agraria and evaluation of their antiproliferative activity against human breast cancer cell lines. Phytothérapie. 2015;14: 10.1007/s10298-015-0981-8.
  • Sadaoui BK, Maiza-Benabdesselam F, Ouadid-Ahidouch H. Antiproliferative Activity of Protopine Extract from Fumaria agraria Against Human Lung Cancer Cell Lines. Phytotherapie. 2021;0293.
  • Yılmaz Sancar P. Analysis of the Essential Oil Composition, Antimicrobial Activity and Antioxidant Capacity of Fumaria asepala Boiss. and Fumaria schleicheri Soy.Will. subsp. microcarpa Hausskn. from Turkey. International Journal of Pure and Applied Sciences, 2023;9(1): 29-37.

In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines

Yıl 2024, , 25 - 29, 28.06.2024
https://doi.org/10.46810/tdfd.1287018

Öz

The Fumaria L. species (Papaveraceae), is known colloquially as “Şahtere”. This species, which has been proven to contain significantly rich components, is used by people to treat a number of different diseases. This study investigated the cytotoxic effects of the methanolic extracts of above-ground portions of Fumaria asepala Boiss. and Fumaria schleicheri Soy.-Will. subsp. microcarpa Boiss. ex Hausskn. taxons against Beas-2B, sh-sy5, HCT116 and A549 cell lines. The changes in cancer cell vitality were identified using the 3-(4.5-dimethylthiazol -2-il)-2.5-diphenyltetrazolium bromide (MTT). While the results showed that F. asepala and F. schleicheri subsp. microcarpa's methanolic extracts presented a meaningful decrease in the vitality of colon (HCT-116) cancer cells, no cytotoxic effect was achieved on lung (A549) cancer and brain (sh-sy5) cancer cells. Also, the herbal extracts did not create any toxic effect on healthy lung cells (Beas-2B).

Kaynakça

  • Aksoy C, Yücecan S, Çiftçi N, Tayfur M, Akgün B, Taşcı N. Local plants used for treatment in cancer disease. J. of Nutr. and Dietetics. 1988;17(1): 111-120.
  • Yılmaz B. Investigation of Antitumoral Activity of Plant Extracts Collected from Various Regions [Master Thesis]. Tekirdağ: Namık Kemal University, Institute of Health Science; 2018.
  • Çoban T, Çitoǧlu G, Sever B, İşcan M. Antioxidant activities of plants used in traditional medicine in Türkiye. Pharmaceutical Biology. 2003;41(8):608-613.
  • Goncalves S, Romano A. The medicinal potential of plants from genus Plantago (Plantaginaceae). Industrial Crops and Products. 2016;83(1):213-226.
  • Baydar H. Tıbbi, Aromatik ve Keyf Bitkileri Bilimi ve Teknolojisi. 1st ed. Türkiye: Suleyman Demirel University Publications; 2005;111-115.
  • Craker LE, Gardner Z, Etter SC. Herbs in American Fields: A Horticultural Perspective of Herband Medical Plant Production in the United Sates. Horticultural Science. 2003;38:977–983.
  • url-1: http://www.turktarim.gov.tr/Haber/64/turkiye-tibbi-ve-aromatik-bitki-yetistiriciliginde-oncu-ulkelerden).
  • Pérez-Gutiérrez MA, Romero-García AT, Salinas MJ, Blanca G, Fernández C, Suárez-Santiago VN. Phylogeny of the tribe Fumarieae (Papaveraceae) based on chloroplast and nuclear DNA sequences: Evolutionary and biogeographic implications. American J. of Bot. 2012;99:517–528.
  • Tutin TG, Burges NA, Chater AO, Edmonson JR, Heywood VH, Moore DM, et al. Psilotaceae to Platanaceae. In Flora Europaea, 2nd ed.; Cambridge, UK: Cambridge University Press; 2010;(1):306–311.
  • Orhan IE, Sener B, Musharraf SG. Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Exp. Toxicol. Pathol. 2012;64:205–209.
  • Sousek J, Guedon D. Alkaloids and organic acids content of Eight Fumaria species. Phytochem Anal. 1999;10:6-11.
  • Paltinean R, Mocan A, Vlase L, Gheldiu AM, Cri¸san G, Ielciu I, et al. Evaluation of Polyphenolic Content, Antioxidant and Diuretic Activities of Six Fumaria Species. Molecules. 2017;22:639-650.
  • Dutta R, Sharma MK, Jha MA. Review on Ethnobotanical, Phytochemistry, Bioactivities and Medicinal Mysteries of Fumaria officinalis (Common Fumitory). EAS J. Pharm. Pharmacol. 2019;1:99–105.
  • Al-Snafi AE. Constituents and Pharmacology of Fumaria officinalis-A Review. IOSR J. Pharm. 2020;10:17–25.
  • Ivanov IG, Vrancheva RZ, Marchev AS, Petkova NT, Aneva IY, Panteley DP, Georgiev VG, Pavlov AI. Antioxidant Activities and Phenolic Compounds in Bulgarian Fumaria Species. Int. J. Curr. Microbiol. Appl. Sci. 2014;3:296–306.
  • Zhang R, Guo Q, Kennelly EJ, Long C, Chai X. Diverse Alkaloids and Biological Activities of Fumaria (Papaveraceae): An Ethnomedicinal Group. Fitoterapia. 2020;146: 104697.
  • Paltinean R, Toiu A, Wauters JN, Frédérich M, Tits M, Angenot L, et al. Identification and Determination of Alkaloids in Fumaria Species from Romania. Dig. J. Nanomater. Biostruct. 2013;8:817–824.
  • Păltinean R, Ielciu I, Hanganu D, Niculae M, Pall E, Angenot L, et al. Biological Activities of Some Isoquinoline Alkaloids from Fumaria schleicheri Soy. Will. Plants. 2022;11(9):1202.
  • Vrancheva R, Ivanov I, Marchev A, Pavlov A. Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method. J. Biosci. Biotechnol. 2012;1:255–259.
  • Çelik AK, Yılmaz Sancar P, Icen Taskın I, Kürşat M. Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines. Artvin Coruh University Journal of Forestry Faculty. 2024;25(1): 104-110.
  • Yener I, Olmez OT, Ertaş A, Yılmaz Ma, Fırat M, Kandemir SI, Öztürk M. A detailed study on chemical and biological profile of nine Euphorbia species from Türkiye with chemometric approach: Remarkable cytotoxicity of E. fistulasa and promising tannic acid content of E. eriophora. Indust. Crops and Prod. 2018;123:442-453.
  • Çelik TA, Aslantürk ÖS, Yılmaz EŞ, Güzel Y. Antioxidant and Cytotoxic Activities of Fumaria parviflora Lam. and Fumaria capreolata L. KSU J. Agric Nat. 2022;25(4):819-827.
  • Tabrizi FH, Irian S, Amanzadeh A, Heidarnejad F, Gudarzi H, Salimi M. Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines. Res Pharm Sci. 2016;11(2):152-9.
  • Seyed RM, Nassiri-Asl M, Farahani-Nick Z, Savad S, Seyed KF. Protective effects of Fumaria vaillantii extract on carbon tetrachloride induced hepatotoxicity in rats. Pharmacologyonline. 2007;3:385-393.
  • Rathi A, Srivastava A, Shirwaikar A, Rawat A. Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine. Phytomedicine. 2008;15:470-477.
  • Hussain T, Hefazat HS, Sheeba F, Sweety K, Vijayakumar M, Rao CV. Chemopreventive effect of Fumaria indica that modulates the oxidant antioxidant inbalance during N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Wistar rats. Asian Pacific J. of Tropical Biomed. 2012;2:995-1001.
  • Ezzat M, Kian A, Bagher M, Seyedeh ZOS. Protective activity of Fumaria vaillantii Loisel and mono methyl fumrate on acetoaminophen induced hepatotoxicity in mice. Int J Pharmacology. 2012;8:177-184.
  • Aveen N, Adham AM, Naqishbandi TE. Cytotoxicity and apoptosis induction by Fumaria officinalis extracts in leukemia and multiple myeloma cell lines. J. of Ethnopharmacology. 2021;266:113458.
  • Sattari R, Khayati GR, Hoshyar R. Biosynthesis and characterization of silver nanoparticles capped by biomolecules by fumaria parviflora extract as green approach and evaluation of their cytotoxicity against human breast cancer MDA-MB-468 cell lines. Materials Chem. and Physics. 2020;241:122438.
  • Aljanaby A. Cytotoxicity of Fumaria officinalis on Leishmania tropica, L20B cell lines and bacteria in vitro. J. of Global Pharma Technology. 2019;11:171-175.
  • Sadaoui BK, Gontier E, Telliez MS, Lequart-Pillon M, Ouadid-Ahidouch H, Maiza F. Characterization of isoquinolin alkaloids from Fumaria agraria and evaluation of their antiproliferative activity against human breast cancer cell lines. Phytothérapie. 2015;14: 10.1007/s10298-015-0981-8.
  • Sadaoui BK, Maiza-Benabdesselam F, Ouadid-Ahidouch H. Antiproliferative Activity of Protopine Extract from Fumaria agraria Against Human Lung Cancer Cell Lines. Phytotherapie. 2021;0293.
  • Yılmaz Sancar P. Analysis of the Essential Oil Composition, Antimicrobial Activity and Antioxidant Capacity of Fumaria asepala Boiss. and Fumaria schleicheri Soy.Will. subsp. microcarpa Hausskn. from Turkey. International Journal of Pure and Applied Sciences, 2023;9(1): 29-37.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Pelin Yılmaz Sancar 0000-0002-6134-622X

Irmak Icen Taskın 0000-0002-1612-0563

Erken Görünüm Tarihi 28 Haziran 2024
Yayımlanma Tarihi 28 Haziran 2024
Yayımlandığı Sayı Yıl 2024

Kaynak Göster

APA Yılmaz Sancar, P., & Icen Taskın, I. (2024). In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines. Türk Doğa Ve Fen Dergisi, 13(2), 25-29. https://doi.org/10.46810/tdfd.1287018
AMA Yılmaz Sancar P, Icen Taskın I. In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines. TDFD. Haziran 2024;13(2):25-29. doi:10.46810/tdfd.1287018
Chicago Yılmaz Sancar, Pelin, ve Irmak Icen Taskın. “In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines”. Türk Doğa Ve Fen Dergisi 13, sy. 2 (Haziran 2024): 25-29. https://doi.org/10.46810/tdfd.1287018.
EndNote Yılmaz Sancar P, Icen Taskın I (01 Haziran 2024) In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines. Türk Doğa ve Fen Dergisi 13 2 25–29.
IEEE P. Yılmaz Sancar ve I. Icen Taskın, “In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines”, TDFD, c. 13, sy. 2, ss. 25–29, 2024, doi: 10.46810/tdfd.1287018.
ISNAD Yılmaz Sancar, Pelin - Icen Taskın, Irmak. “In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines”. Türk Doğa ve Fen Dergisi 13/2 (Haziran 2024), 25-29. https://doi.org/10.46810/tdfd.1287018.
JAMA Yılmaz Sancar P, Icen Taskın I. In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines. TDFD. 2024;13:25–29.
MLA Yılmaz Sancar, Pelin ve Irmak Icen Taskın. “In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines”. Türk Doğa Ve Fen Dergisi, c. 13, sy. 2, 2024, ss. 25-29, doi:10.46810/tdfd.1287018.
Vancouver Yılmaz Sancar P, Icen Taskın I. In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines. TDFD. 2024;13(2):25-9.