Evaluation of the Effect of Different Extraction Temperatures on the Synthesis of Silver Nanoparticles from Ocimum basilicum (Basil) Plant

Yıl 2024, , 88 - 94, 28.06.2024

İlkay Ünal , Burcu Aydoğdu

https://doi.org/10.46810/tdfd.1454698

Öz

The eco-friendly green synthesis of silver nanoparticles (AgNPs) using Ocimum basilicum (basil) extract at varying extraction temperatures (40°C, 60°C, 80°C, 100°C) was investigated to determine the optimal conditions for nanoparticle formation. Analysis methods such UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and TransmissionElectron Microscopy (TEM) confirmed the crystalline, spherical nature of AgNPs and identified phytochemicals acting as capping and reducing agents. Notably, the extraction temperature was found to influence both the DPPH radical scavenging activity and the structural properties of AgNPs. According to TEM analysis results, it was observed that high extraction temperatures increased the nanoparticle formation efficiency but created a wide size distribution. The crystallite sizes, calculated using the Scherrer equation, for AgNPs synthesized at different extraction temperatures, were determined to be 12.45 nm, 18.77 nm, 17.76 nm, and 16.03 nm, respectively. The hydrodynamic sizes of the AgNPs were found to range between 158.1 and 333.7 nm. The study highlights the critical role of extraction temperature in the synthesis process, suggesting 40°C as the optimal temperature for achieving efficient and environmentally friendly synthesis of AgNPs with enhanced biological activities.

Anahtar Kelimeler

Green Synthesis, extraction, silver nanoparticle, Temparature, Ocimum basilicum

Etik Beyan

Çalışmanın her aşamasında, bilimsel araştırma ve yayın etiğine uygun davranılmıştır. Araştırmacılar, çalışmanın planlanması, uygulanması ve raporlanması sırasında her türlü sahtecilik, çıkar çatışması, veri manipülasyonu gibi etik olmayan davranışlardan kaçınmışlard

Teşekkür

Yazarlara katkılarından dolayı teşekkür ederiz

Ocimum basilicum (Fesleğen) Bitkisinin Farklı Ekstraksiyon Sıcaklıklarının Gümüş Nanopartikül Sentezine Etkisinin Değerlendirilmesi

Öz

Ocimum basilicum (fesleğen) özütü kullanılarak çeşitli ekstraksiyon sıcaklıklarında (40, 60, 80, ve 100°C) çevre dostu yeşil sentez yöntemi ile gümüş nanopartikülleri (AgNPs) sentezi araştırıldı. Nanopartikül oluşumunun optimal koşullarını belirlemek için UV-Vis spektrofotometri, Fourier Dönüşümlü Kızılötesi Spektroskopi (FTIR), X-ışını Kırınımı (XRD) ve Geçirimli Elektron Mikroskobu (TEM) gibi analiz yöntemleri kullanıldı. Bu yöntemlerle AgNPs'lerin kristalin ve küresel yapısı doğrulanmış ve kaplama ile indirgeyici ajan olarak hareket eden fitokimyasallar tespit edilmiştir. Özellikle, ekstraksiyon sıcaklığının hem DPPH radikal süpürme aktivitesini hem de AgNPs'nin yapısal özelliklerini etkilediği bulunmuştur. TEM analizi, daha yüksek ekstraksiyon sıcaklıklarının nanopartikül oluşum verimliliğinin artmasına yol açtığını ancak aynı zamanda daha geniş boyut dağılımına yol açtığını ortaya çıkardı. Farklı ekstraksiyon sıcaklıklarında sentezlenen AgNPs'lerin kristalit boyutları Scherrer denklemi kullanılarak sırasıyla 12.45 nm, 18.77 nm, 17.76 nm ve 16.03 nm olarak hesaplanmıştır. AgNPs'nin hidrodinamik boyutlarının 158.1 ile 333.7 nm arasında değişmiştir. Çalışma, ekstraksiyon sıcaklığının sentez sürecindeki kritik rolünü vurgulayarak, gelişmiş biyolojik aktivitelere sahip AgNP'lerin verimli ve çevre dostu sentezini için 40 °C’yi en uygun ekstraksiyon sıcaklığı olarak önermektedir.

Anahtar Kelimeler

Yeşil sentez, Ekstraksiyon, Ocimum basilicum, Gümüş nanopartikül

Etik Beyan

Throughout all stages of the study, actions were taken in strict adherence to the ethics of scientific research and publication. The researchers have avoided any form of misconduct, such as fraud, conflicts of interest, or data manipulation, during the planning, execution, and reporting of the study.

Teşekkür

We thank the authors for their contributions

Kaynakça

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