Use of L-Lactate Dehydrogenase Immobilized on Carboxylated Multiwalled Carbon Nanotubes/Polyaniline/Pencil Graphite Electrode as a Lactate Biosensor

Öz

In this study, L-Lactate dehydrogenase (L-LDH) was covalently immobilized on carboxylated multiwalled carbon nanotubes (cMWCNT)/polyaniline (PANI)/pencil graphite electrode (PGE) and used as a lactate biosensor. Electrochemical polymerization of PANI was carried out using a three-electrode cell technique via cyclic voltammretry (CV). The characterization of LDH/cMWCNT/PANI/PGE electrode was achieved using electrochemical and scanning electron microscopy (SEM) techniques. The effect of applied potential, pH, lactate concentration, and NAD+ (with and without) on sensor response was assessed. The optimal pH was determined as 7.0. Biosensor response increased with increasing of lactate concentration. The current values were obtained as 0.026 and 0.038 mA cm-2 for 0.166 and 1.331 mM lactate solution with NAD+ as a cofactor, respectively at +0.2 V. The results indicate that LDH/cMWCNT/PANI/PGE biosensor was more sensitive than the biosensor without carbon nanotubes.

Anahtar Kelimeler

• Biosensor
• Lactate Dehydrogenase
• Pencil Graphite Electrode
• Carbon Nanotubes

Use of L-Lactate Dehydrogenase Immobilized on Carboxylated Multiwalled Carbon Nanotubes/Polyaniline/Pencil Graphite Electrode as a Lactate Biosensor

Öz

Bu çalışmada, L-Laktat dehidrojenaz (L-LDH), karboksillenmiş çok duvarlı karbon nanotüpler (cMWCNT)/polianilin (PANI)/kalem grafit elektrot (PGE) üzerinde kovalent olarak immobilize edilmiş ve laktat biyosensörü olarak kullanılmıştır. PANI’ nin elektrokimyasal polimerizasyonu, dönüşümlü voltametri (CV) ile üç elektrot tekniği kullanılarak gerçekleştirildi. LDH/cMWCNT/PANI/PGE elektrotunun karakterizasyonu, taramalı elektron mikroskobu (SEM) ve elektrokimyasal deneylerle yapılmıştır. Uygulanan potansiyel, pH ve laktat derişiminin NAD+ (ile ve olmadan) sensör yanıtı üzerine etkileri araştırılmıştır. Optimum pH 7,0 olarak belirlendi. Biyosensör yanıtının, laktat derişimi ile birlikte arttığı belirlendi. Akım değerleri, kofaktör olarak NAD+ ile +0,2 V’ ta 0,166 ve 1,331 mM laktat çözeltisi için sırasıyla 0,026 ve 0,038 mA cm-2 olarak belirlendi. Sonuçlar, LDH/cMWCNT/PANI/PGE biyosensörün, karbon nanotüp kullanılmayan biyosensöre göre daha duyarlı olduğunu göstermiştir.

Anahtar Kelimeler

• Biyosensör
• Laktat Dehidrojenaz
• Kalem Grafit Elektrot
• Karbon Nanotüpler

Kaynakça

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