Comparison of Anticancer Capacity of Six Grape Extracts on Cancerous Liver Cell Line

Yıl 2025, Cilt: 14 Sayı: 4, 85 - 89, 30.12.2025

Laila Elfogohi , Oktay Bıyıklıoğlu , Aslı Uğurlu Bayarslan , Serhat Karabıcak , İdris Yazgan , Talip Çeter

https://doi.org/10.46810/tdfd.1712868

Öz

Grape is one of the most widely consumed food sources worldwide and has been under massive research for its health benefits. In this study, we used ethanolic extracts from six different grape cultivars (both white and black grapes) to test their anticancer capacity for Huh7 liver cancer cell line. The findings showed that the grape cultivar species play the role on the cytotoxicity, which was observed as biphasic dose dependent. Based on the findings and the literature, it can be claimed rather than using whole grape extracts, fractionation of the extracts is needed to isolate bioactive compounds due to the fact that flavonoids can serve as cytotoxicity and cytoprotective behaviors depending on their chemistry.

Anahtar Kelimeler

Grape extract , Huh7 cell line , Cancer , Cytotoxicity , Flavonoids

Altı Çeşit Üzüm Ekstraktının Kanserli Karaciğer Hücre Hattı Üzerindeki Antikanser Kapasitesinin Karşılaştırılması

Öz

Üzüm, dünya çapında en yaygın tüketilen gıda kaynaklarından biridir ve sağlık yararları için kapsamlı araştırmalara tabi tutulmaktadır. Bu çalışmada, Huh7 karaciğer kanseri hücre hattı için antikanser kapasitelerini test etmek amacıyla altı farklı üzüm çeşidinden (hem beyaz hem de siyah üzüm) etanolik özütler kullanıldı. Bulgular, üzüm çeşidi türlerinin sitotoksisitede rol oynadığını ve bunun da iki fazlı doz bağımlı olarak gözlemlendiğini gösterdi. Bulgulara ve literatüre dayanarak, flavonoidlerin kimyalarına bağlı olarak sitotoksisite ve sitoprotektif davranışlarda bulunabilmeleri nedeniyle, biyoaktif bileşikleri izole etmek için bütün üzüm özütleri kullanmak yerine özütlerin fraksiyonlanmasının gerekli olduğu iddia edilebilir.

Kaynakça

• Signorelli P, Fabiani C, Brizzolari A, Paroni R, Casas J, Fabriàs G, et al. Natural Grape Extracts Regulate Colon Cancer Cells Malignancy. Nutrition and Cancer. 2015; 67(3):494–503.
• Morré DM, Morré DJ. Anticancer activity of grape and grape skin extracts alone and combined with green tea infusions. Cancer Letters. 2006; 238(2):202–9.
• Sahpazidou D, Geromichalos GD, Stagos D, Apostolou A, Haroutounian SA, Tsatsakis AM, et al. Anticarcinogenic activity of polyphenolic extracts from grape stems against breast, colon, renal and thyroid cancer cells. Toxicology Letters. 2014; 230(2):218–24.
• Habib HM, El-Fakharany EM, Kheadr E, Ibrahim WH. Grape seed proanthocyanidin extract inhibits DNA and protein damage and labile iron, enzyme, and cancer cell activities. Scientific Reports. 2022; 12(1).
• Dinicola S, Pasqualato A, Cucina A, Coluccia P, Ferranti F, Canipari R, et al. Grape seed extract suppresses MDA-MB231 breast cancer cell migration and invasion. European Journal of Nutrition. 2013; 53(2):421–31.
• Singh T, Sharma SD, Katiyar SK. Grape Proanthocyanidins Induce Apoptosis by Loss of Mitochondrial Membrane Potential of Human Non-Small Cell Lung Cancer Cells In Vitro and In Vivo. PLoS ONE. Public Library of Science; 2011; 6(11):e27444–4.
• Dinicola S, Cucina A, Pasqualato A, D’Anselmi F, Proietti S, Lisi E, et al. Antiproliferative and Apoptotic Effects Triggered by Grape Seed Extract (GSE) versus Epigallocatechin and Procyanidins on Colon Cancer Cell Lines. International Journal of Molecular Sciences. 2012; 13(1):651–64.
• Zhou K, Raffoul JJ. Potential Anticancer Properties of Grape Antioxidants. Journal of Oncology. 2012; 2012:1–8.
• Laila Radwan Abdalla E, Tepe HD, Yazgan İ, Çeter T. Chemical composition and antioxidant activity of seven different grape extracts. Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering. 2018; 27(2):47–54.
• Mete D, Taşlıca EY, Mohamed GŞ. Sorafenib loaded ZIF-8 metal-organic frameworks as a multifunctional nano-carrier offers effective hepatocellular carcinoma therapy. Journal of Drug Delivery Science and Technology. Elsevier BV; 2023; 82:104362–2.
• Caddeo C, Nigro I, Chiummiento L, Funicello M, Lupattelli P, Santarsiere A, et al. A novel aminohydroxy sulfonamide formulated in PEGylated liposomes with potential antitumor activity. Journal of Drug Delivery Science and Technology. Elsevier BV; 2025; 106:106739.
• Sancak S, Yazgan İ, Bayarslan AU, Ayna A, Evecen S, Taşdelen Z, et al. Surface chemistry dependent toxicity of inorganic nanostructure glycoconjugates on bacterial cells and cancer cell lines. Journal of Drug Delivery Science and Technology. Elsevier BV; 2022; 79:104054–4.
• Liang Z, Cheng L, Zhong G-Y, Liu RH. Antioxidant and Antiproliferative Activities of Twenty-Four Vitis vinifera Grapes. PLoS ONE. 2014; 9(8):e105146.
• Ferhi S, Santaniello S, Zerizer S, Cruciani S, Fadda A, Sanna D, et al. Total Phenols from Grape Leaves Counteract Cell Proliferation and Modulate Apoptosis-Related Gene Expression in MCF-7 and HepG2 Human Cancer Cell Lines. Molecules. 2019; 24(3):612.
• Milella RA, Rosso MD, Gasparro M, Gigante I, Debiase G, Forleo LR, et al. Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.). Frontiers in Plant Science. Frontiers Media; 2023; 13.
• Esparza I, Moler JA, Arteta M, Moreno NJ, Ancín-Azpilicueta C. Phenolic Composition of Grape Stems from Different Spanish Varieties and Vintages. Biomolecules. Multidisciplinary Digital Publishing Institute; 2021; 11(8):1221–1.
• Colombo F, Di Lorenzo C, Regazzoni L, Fumagalli M, Sangiovanni E, Peres de Sousa L, et al. Phenolic profiles and anti-inflammatory activities of sixteen table grape (Vitis vinifera L.) varieties. Food & Function. 2019; 10(4):1797–807.
• Di Lorenzo C, Colombo F, Biella S, Orgiu F, Frigerio G, Regazzoni L, et al. Phenolic profile and antioxidant activity of different grape (Vitis vinifera L.) varieties. BIO Web of Conferences. 2019; 12:04005.
• Pyo Y, Kwon KH, Jung YJ. Anticancer Potential of Flavonoids: Their Role in Cancer Prevention and Health Benefits. Foods. Multidisciplinary Digital Publishing Institute; 2024; 13(14):2253–3.
• Vazhappilly CG, Alsawaf S, Mathew S, Nasar NA, Hussain MI, Cherkaoui NM, et al. Pharmacodynamics and safety in relation to dose and response of plant flavonoids in treatment of cancers. Inflammopharmacology. Springer Nature; 2024.