Investigation of Water Quality and Molecular Analysis (16s rRNA) of Bacterial Community Structure in a Rural Geothermal Spring: Ucler Hill (Azat Village), Kars, Türkiye

Öz

This study was carried out to evaluate the chemical and microbiological properties of a natural water source located in Azat Village of Kars province and used as drinking water by the surrounding villagers. Water samples taken from three different sampling points were analyzed for pH, electrical conductivity, total hardness, alkalinity, nitrogenous compounds (NO₂-, NO₃-, NH₄⁺), metal contents (Fe, Cu, Al, Pb), sulfate and chloride levels. The data obtained were compared with World Health Organization (WHO) drinking water standards and one-sample t-test was applied for each parameter. Statistically significant differences were found in total hardness, NO₃-, Fe, Cu, SO₄²- and Cl- parameters (p < 0.05), while other parameters were found to be compatible with WHO reference values. In microbiological analyses, a total of eight different bacterial species were isolated by culture-dependent methods. These species were identified by 16S rRNA gene sequencing analysis and all of them were determined to be non-pathogenic environmental microorganisms. The identified species included Microbacterium spp., Bacillus spp., Corynebacterium spp., Lactobacillus spp., Rhodococcus spp., Pseudomonas spp., Leuconostoc spp. and Aeromonas spp. Although some species have the potential to be opportunistic pathogens, the overall microbiological profile was considered to be within acceptable limits for natural sources.

Anahtar Kelimeler

• : Natural drinking water source
• Microbial diversity
• Rural water security
• Chemical water quality
• 16S rRNA sequence analysis

Kırsal Bir Jeotermal Kaynakta Su Kalitesi ve Moleküler Analiz (16s rRNA) ile Bakteriyel Topluluk Yapısının İncelenmesi: Üçler Tepesi (Azat Köyü), Kars, Türkiye

Öz

Bu çalışma, Kars ilinin Azat Köyü'nde bulunan ve çevre köylüler tarafından içme suyu olarak kullanılan doğal bir su kaynağının kimyasal ve mikrobiyolojik özelliklerini değerlendirmek amacıyla gerçekleştirilmiştir. Üç farklı örnekleme noktasından alınan su örnekleri pH, elektriksel iletkenlik, toplam sertlik, alkalinite, azotlu bileşikler (NO₂-, NO₃-, NH₄⁺), metal içerikleri (Fe, Cu, Al, Pb), sülfat ve klorür seviyeleri açısından analiz edilmiştir. Elde edilen veriler Dünya Sağlık Örgütü (WHO) içme suyu standartları ile karşılaştırılmış ve her parametre için tek örneklem t-testi uygulanmıştır. Toplam sertlik, NO₃-, Fe, Cu, SO₄²- ve Cl- parametrelerinde istatistiksel olarak anlamlı farklılıklar bulunurken (p<0.05), diğer parametreler WHO referans değerleriyle uyumlu bulunmuştur. Mikrobiyolojik analizlerde, kültüre bağlı yöntemlerle toplam sekiz farklı bakteri türü izole edilmiştir. Bu türler 16S rRNA gen dizileme analizi ile tanımlanmış ve hepsinin patojen olmayan çevresel mikroorganizmalar olduğu belirlenmiştir. Tanımlanan türler arasında Microbacterium spp., Bacillus spp., Corynebacterium spp., Lactobacillus spp., Rhodococcus spp., Pseudomonas spp., Leuconostoc spp. ve Aeromonas spp. yer almaktadır. Bazı türler fırsatçı patojen olma potansiyeline sahip olsa da, genel mikrobiyolojik profilin doğal kaynaklar için kabul edilebilir sınırlar içinde olduğu düşünülmüştür.

Anahtar Kelimeler

• Doğal içme suyu kaynağı
• Mikrobiyal çeşitlilik
• Kırsal su güvenliği
• Kimyasal su kalitesi
• 16S rRNA dizi analizi

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