AZD-1390, Yumurtalık Kanseri Hücre Hatlarında Hücre Hareketliliğini Bozar ve ROS Tetiklemeli Apoptotik Hücre Ölümüne Neden Olur

Öz

Ataksi Telanjiektazi mutasyonu (ATM), serin/treonin kinaz için bir protein kodlayan bir gendir. ATM'nin fosforilasyonu, genomik bütünlüğü koruyarak tümör oluşumunu önler. ATM genindeki somatik mutasyonlar bazı yumurtalık kanseri tiplerinde tespit edilmiştir. Bu nedenle, yumurtalık kanseri hücrelerinde AZD-1390 ile ATM inhibisyonunun antikanser etkisini test ettik. Sonuçlarımız, AZD-1390'ın yumurtalık kanseri hücrelerinde doz ve zamana bağlı bir şekilde hücre canlılığını azaltarak antiproliferatif bir etki gösterdiğini gösterdi. Daha ileri analizler, AZD-1390'ın göç potansiyelini önemli ölçüde inhibe ettiğini ve ROS aktivitesini ve hücre ölümünü artırdığını ortaya koydu. Bulgularımız birlikte, AZD-1390 ile ATM inhibisyonunun yumurtalık kanseri hücrelerini tedavi etmede etkili olduğunu ve daha ileri araştırmaların bu çalışmaya katkıda bulunacağını göstermektedir.

Anahtar Kelimeler

• ATM
• AZD-1390
• Yumurtalık Kanseri
• Hücre Proliferasyonu

AZD-1390 Impairs Cell Mobility and Induces ROS-triggered Apoptotic Cell Death in Ovarian Cancer Cell Lines

Öz

The Ataxia Telangiectasia mutation (ATM) codes for a protein for serine/threonine kinase. Phosphorylation of ATM prevents tumor formation by preserving genomic integrity. Somatic mutations in the ATM gene have been identified in some types of ovarian cancer. Therefore, in this study, we tested the anticancer effects of ATM inhibition with AZD-1390 in ovarian cancer cells to eliminate these mutations. Our results showed that AZD-1390 exhibited an antiproliferative effect by decreasing cell viability in ovarian cancer cells in a dose- and time-dependent manner. Further analysis revealed that AZD-1390 significantly inhibited the migration potential and increased ROS activity and apoptotic cell death. Our findings together suggest that ATM inhibition with AZD-1390 is effective in treating ovarian cancer cells and further investigation will contribute to this study.

Anahtar Kelimeler

• ATM
• AZD-1390
• Ovarian Cancer
• Cell Proliferation.

Kaynakça

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