Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri

Selim Çomaklı; Mustafa İleritürk; Esra Manavoğlu Kirman

doi:10.46810/tdfd.809231

Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri

Öz

Kolistin, gram-negatif bakterilerin neden olduğu enfeksiyonlarda son seçenek olarak tedavi amacıyla kullanılmaktadır. Literatürde kolistinin neden olduğu testis toksisitesi bildirilmiş olmasına rağmen mekanizmasına ilişkin çok az veri bulunmaktadır. Yapılan bu çalışmanın amacı kolistin uygulamasının neden olduğu testis toksisitesinde rutinin (Rut) koruyucu etkilerinin araştırılmasıdır. Bu amaç için 5 adet deneysel grup oluşturuldu; Kontrol, Rut (100 mg/kg/gün), Kolistin (15 mg/kg/gün), Kolistin + Rut (50 mg/kg/gün), Kolistin + Rut (100 mg/kg/gün). Uygulama sonrasında ratların testis dokularında malondialdehit (MDA) ve redükte glutatyon (GSH) seviyeleri tespit edildi. Aynı zamanda testis dokularının histopatolojik incelemesi yapıldı ve 8-hidroksi-2'-deoksiguanozin (8-OHdG), nükleer faktörü kappa B (NF-κB) ve kaspaz 3 aktiviteleri immunohistokimya ile belirlendi. Sonuçlara bakıldığında kolistin uygulamasının rat testis dokularında MDA düzeyini arttırdığı, GSH düzeyini ise azalttığı tespit edilmiştir. Farklı dozlarda Rut uygulaması sonrası ise MDA düzeyinde azalma, GSH düzeyinde ise artış gözlendi. Kolistinin testis dokularında neden olduğu hasar sonucu 8-OHdG, NF-κB ve kaspaz 3 immunpozitifliklerinin arttığı, özellikle Rut (100 mg/kg/gün) uygulamasından sonra bu proteinlerin aktivite düzeylerinin azaldığı belirlendi. Sonuç olarak kolistinin rat testislerinde oksidatif hasar, enflamasyon, DNA hasarı ve apoptoza neden olduğu, Rut uygulamasının kolistin kaynaklı testis hasarında koruyucu etki gösterdiği belirlendi.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Veteriner Cerrahi

Bölüm

Araştırma Makalesi

Yazarlar

Selim Çomaklı ^*
0000-0002-8744-7686
Türkiye

Mustafa İleritürk
0000-0002-4581-4492
Türkiye

Esra Manavoğlu Kirman Bu kişi benim
0000-0003-3877-7686
Türkiye

Yayımlanma Tarihi

30 Aralık 2020

Gönderilme Tarihi

12 Ekim 2020

Kabul Tarihi

16 Kasım 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 9 Sayı: 2

DOI

https://doi.org/10.46810/tdfd.809231

IZ

https://izlik.org/JA77GC74DY

Kaynak Göster

RIS / Bibtex

APA

Çomaklı, S., İleritürk, M., & Manavoğlu Kirman, E. (2020). Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. Türk Doğa ve Fen Dergisi, 9(2), 83-90. https://doi.org/10.46810/tdfd.809231

AMA

1.Çomaklı S, İleritürk M, Manavoğlu Kirman E. Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. TDFD. 2020;9(2):83-90. doi:10.46810/tdfd.809231

Chicago

Çomaklı, Selim, Mustafa İleritürk, ve Esra Manavoğlu Kirman. 2020. “Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri”. Türk Doğa ve Fen Dergisi 9 (2): 83-90. https://doi.org/10.46810/tdfd.809231.

EndNote

Çomaklı S, İleritürk M, Manavoğlu Kirman E (01 Aralık 2020) Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. Türk Doğa ve Fen Dergisi 9 2 83–90.

IEEE

[1]S. Çomaklı, M. İleritürk, ve E. Manavoğlu Kirman, “Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri”, TDFD, c. 9, sy 2, ss. 83–90, Ara. 2020, doi: 10.46810/tdfd.809231.

ISNAD

Çomaklı, Selim - İleritürk, Mustafa - Manavoğlu Kirman, Esra. “Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri”. Türk Doğa ve Fen Dergisi 9/2 (01 Aralık 2020): 83-90. https://doi.org/10.46810/tdfd.809231.

JAMA

1.Çomaklı S, İleritürk M, Manavoğlu Kirman E. Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. TDFD. 2020;9:83–90.

MLA

Çomaklı, Selim, vd. “Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri”. Türk Doğa ve Fen Dergisi, c. 9, sy 2, Aralık 2020, ss. 83-90, doi:10.46810/tdfd.809231.

Vancouver

1.Selim Çomaklı, Mustafa İleritürk, Esra Manavoğlu Kirman. Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. TDFD. 01 Aralık 2020;9(2):83-90. doi:10.46810/tdfd.809231

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Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi

https://doi.org/10.62425/jlasp.1569011