Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri

Yıl 2020, , 83 - 90, 30.12.2020

Selim Çomaklı , Mustafa İleritürk , Esra Manavoğlu Kirman

https://doi.org/10.46810/tdfd.809231

Öz

Kolistin, gram-negatif bakterilerin neden olduğu enfeksiyonlarda son seçenek olarak tedavi amacıyla kullanılmaktadır. Literatürde kolistinin neden olduğu testis toksisitesi bildirilmiş olmasına rağmen mekanizmasına ilişkin çok az veri bulunmaktadır. Yapılan bu çalışmanın amacı kolistin uygulamasının neden olduğu testis toksisitesinde rutinin (Rut) koruyucu etkilerinin araştırılmasıdır. Bu amaç için 5 adet deneysel grup oluşturuldu; Kontrol, Rut (100 mg/kg/gün), Kolistin (15 mg/kg/gün), Kolistin + Rut (50 mg/kg/gün), Kolistin + Rut (100 mg/kg/gün). Uygulama sonrasında ratların testis dokularında malondialdehit (MDA) ve redükte glutatyon (GSH) seviyeleri tespit edildi. Aynı zamanda testis dokularının histopatolojik incelemesi yapıldı ve 8-hidroksi-2'-deoksiguanozin (8-OHdG), nükleer faktörü kappa B (NF-κB) ve kaspaz 3 aktiviteleri immunohistokimya ile belirlendi. Sonuçlara bakıldığında kolistin uygulamasının rat testis dokularında MDA düzeyini arttırdığı, GSH düzeyini ise azalttığı tespit edilmiştir. Farklı dozlarda Rut uygulaması sonrası ise MDA düzeyinde azalma, GSH düzeyinde ise artış gözlendi. Kolistinin testis dokularında neden olduğu hasar sonucu 8-OHdG, NF-κB ve kaspaz 3 immunpozitifliklerinin arttığı, özellikle Rut (100 mg/kg/gün) uygulamasından sonra bu proteinlerin aktivite düzeylerinin azaldığı belirlendi. Sonuç olarak kolistinin rat testislerinde oksidatif hasar, enflamasyon, DNA hasarı ve apoptoza neden olduğu, Rut uygulamasının kolistin kaynaklı testis hasarında koruyucu etki gösterdiği belirlendi.

Anahtar Kelimeler

Apoptoz, Enflamasyon, Kolistin, Oksidatif stress, 8-OHdG, Testis

Kaynakça

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