The Investigation of PestivirusInfections in Bovine, Ovine and Caprine Foetuses in the East and Southeast Regions of Turkey

Yıl 2022, , 1 - 5, 25.03.2022

Metin Gürçay

https://doi.org/10.46810/tdfd.850555

Öz

The present study was aimed to investigate the presence of pestiviruses in bovine, ovine and caprine foetuses in the East and Southeast regions of Turkey by antigenenzyme-linked immunosorbent assay (Ag-ELISA) method. For this purpose, 245 bovine foetuses, 89 ovine foetuses and caprine foetuses (in total 370 foetuses) were collected from Elazig, Malatya, Tunceli, Bingöl, Bitlis, Diyarbakır, Şırnak and Hakkâriprovinces during January-May 2012. A pool of foetal tissues (lung, liver and spleen) was created for each foetus. The sample of each foetus was tested with the Ag-ELISA test. Pestivirus antigens were detected in 81 of 245 cattle samples (33%), 23 of 89 sheep samples (25.8%), and 8 of 36 goat samples (22.2%). According to these results, it has been revealed that pestivirus infectionis responsible for abortion in cattle, sheep and goats in the investigated regions.

Anahtar Kelimeler

Pestivirus, Aborted Foetuses, The East and SoutheastAnatolia, Turkey

Türkiye’nin Doğu ve Güneydoğu Bölgelerinde Abort Yapmış Sığır, Koyun ve Keçi Fötuslarında Pestivirus Enfeksiyonunun Araştırılması

Öz

Bu çalışmada, Doğu ve Güneydoğu Anadolu bölgelerinde bulunan illerde yetiştirilen sığır, koyun ve keçilerin abort olmuş fötuslarında pestivirus antijen varlığının ticari bir ELISA (AgELISA,IDEXX Laboratories,Inc,Maine,USA) testi ile araştırılması amaçlanmıştır. Elâzığ, Malatya, Tunceli, Bingöl, Bitlis, Diyarbakır, Şırnak ve Hakkâriillerinden 2012 yılı, Ocak-Mayıs ayları arasında 245 sığır, 89 koyun, 36 keçi olmak üzere toplam 370 aborte olmuş fötus toplandı. Her bir fötus için, akciğer, karaciğer ve dalak doku numunelerini içeren, bir numune havuzu oluşturuldu. Her bir fötuse ait numune, Ag-ELISA testi ile test edildi. Test sonucunda, 245 sığır numunesinin 81’inde (%33), 89 koyun numunesinin 23’ünde (%25,8) ve 36 keçi numunesinin 8’inde (%22,2) pestivirus antijeni tespit edildi. Bu sonuçlara göre, araştırmanın yapıldığı bölgelerde sığır, koyun ve keçilerde abort yapma nedeni olarak önemli derecede pestivirus enfeksiyonunun sorumlu olduğu ortaya konulmuş oldu.

Anahtar Kelimeler

Pestivirüs, Abort Yapmış Fötüs, Doğu ve Güneydoğu Anadolu, Türkiye

Kaynakça

• [1]Smith DB, Meyers G, Bukh J, Gould EA, Monath T, Muerhoff, A.S, Pletnev A, Rico-Hesse R, Stapleton JT, Simmonds P, Becher P. Proposed revision to the taxonomy of the genus Pestivirus, family Flaviviridae.The Journal of general virology2017; 98 (8), 2106.
• [2]Simmonds P, Becher P, Bukh J, Gould EA, Meyers G, Monath T, Muerho S, Pletnev A, Rico-Hesse R, Smith DB et al. ICTV Virus Taxonomy Profile: Flaviviridae. J. Gen. Virol. 2017; 98: 2–3.
• [3]Yesilbag K, Alpay G, Becher P. Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus. Viruses 2017; 9: 128.
• [4]Bauermann FV, Ridpath JF, Weiblen R, Flores EF. HoBi-like viruses: An emerging group of pestiviruses. J. Veter. Diagn. Investig. 2013;25:6–15.
• [5]Tao J, Liao J, Wang Y, Zhang X, Wang J, Zhu G. Bovine viral diarrhea virus (BVDV) infections in pigs. Veter. Microbiol. 2013; 165:185–189.
• [6]Braun U, Hilbe M, Peterhans E, Schweizer M. Border disease in cattle. Veter. J. 2019;246: 12–20.
• [7]Pinior B, Garcia S, Minviel JJ, Raboisson D. Epidemiological factors and mitigation measures influencing production losses in cattle due to bovine viral diarrhoea virus infection: A meta‐analysis.Transboundary and emerging diseases,2019; 66(6): 2426-2439.
• [8]Kelling CL. Viral diseases of the fetus. In: Youngquist RS, Threlfall WR, Editors. Current therapy in large animal therio-genology. 2nd ed. St. Louis: Elsevier 399–408. 2007.
• [9]Givens MD, Marley MS, Infectious causes of embryonic and fetalmortality. Theriogenology. 2008; 70: 270–285.
• [10]Adu-Addai B, Koney EB, Addo P, Kaneene J, Mackenzie C, Agnew DW, Importance of infectious bovine reproductive diseases: an example from Ghana. Veterinary record. 2012; 171: 47-48.
• [11]Anderson ML. Infectious causes of bovine abortion during midto late-gestation. Theriogenology. 2007; 68: 474-86.
• [12]Yang N, Cui X, Qian W, Yu S, Liu Q. Survey of nine abortifacient infectious agents in aborted bovine fetuses fromdairy farms in Beijing, China by PCR. Acta Veterinaria Hungarica. 2012; 60: 83-92.
• [13]Borel N, Frey CF, Gottstein B, Hilbe M, Pospischil A, Franzoso F D, Waldvogel A. Laboratory diagnosis of ruminant abortion in Europe.The Veterinary Journal,2014; 200 (2): 218-229.
• [14]Alpay G, Öner EB, Yeşilbağ K.Seroepidemiology and molecular investigation of pestiviruses among sheep and goats in Northwest Anatolia.Turkish Journal of Veterinary and Animal Sciences, 2018;42(3), 205-210.
• [15]Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine. Tenth Edition. Edinburgh, London, New York, Oxford, Philadelphia, St Louis, Sydney, Toronto: Saunders Elsevier, 1248-77, 2008.
• [16]Burgu Ġ, Alkan F, Özkul A, ve ark. Türkiye'de süt sığırcılığı işletmelerinde bovine viral diarrhoea virus (BVDV) enfeksiyonunun epidemiyolojisi ve kontrolü. Ankara Üniv. Vet. Fak. Derg.2003; 50: 127-133.
• [17]Tan MT, Karaoğlu MT, Erol N, et al. Serological andvirological investigations of bovine viral diarrhoea virus (BVDV) infection in dairy cattle herds in Aydın province. Turkish Journal of Veterinary and Animal Sciences. 2006; 30(3): 299-304.
• [18]Yildirim Y, Yilmaz V, Kalaycioglu AT et al. An investigation of a possible involvement of BVDV, BHV-1 and BHV-4 infections in abortion of dairy cattle in Kars district of Turkey. Kafkas Univ. Vet. Fak. Derg. 2011; 17: 879-883.
• [19]Gürçay M, İssi M, Gül Y. Investigation of bovine viral diarrhoea virus in dairy cattle premises where aborts occur. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2013; 10 (2) : 87-91.
• [20]Krametter-Froetscher R, Duenser M, Preyler B,Theiner A, Benetka V, Moestl K, Baumgartner W. Pestivirus infection in sheep and goats in West Austria. Vet J. 2010; 186: 342-346.
• [21]Dehkordi FS. Prevalence study of Bovine viral diarrhea virus by evaluation of antigen capture ELISA and RT-PCR assay in Bovine, Ovine, Caprine, Buffalo and Camel aborted fetuses in Iran.AMB express. 2011;1(1): 1-6.
• [22]Albayrak H, Gumusova SO, Ozan E, Yazici Z. Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey.Tropical animal health and production. 2012; 44 (4): 677-680.
• [23]Şevik M. Genomic characterization of pestiviruses isolated from bovine, ovine andcaprine foetuses in Turkey: A potentially new genotype of pestivirus I species.Transboundary and Emerging Diseases. 2020;00:1-10.
• [24]Gürçay M, Keçeci H, Öztürk M. Determination of presence and prevalence of Bovine Viral Diarrhea Virus infection in cattle herds in Bingol province.Etlik Veteriner Mikrobiyoloji Dergisi, 31(1), 34-38.