Bazı Çiçek Balların Botanik Çeşitliliği, Antioksidan ve Antimikrobiyal Aktivitesi

Öz

Çalışma, Türkiye'de toplanan dört bal örneğinin botanik kökenini, antioksidan güç, antimikrobiyal aktivite ve fizikokimyasal özelliklerini değerlendirmek amacıyla planlandı. Melissopalinolojik sonuçlar, Örnek 1 ve Örnek 2'nin polifloral, diğerlerinin ise unifloral olduğunu ortaya çıkardı. Toplam fenolik (116.47- 1711.13 mgGAE/100 g) ve flavonoid (1.46- 27.25 mgCAE/100 g) miktarları belirlendi. Ayrıca, antioksidan aktiviteler demir iyon şelatlama aktivitesi (FICA) (% 71.32-93.35), hidrojen peroksit giderme aktivitesi (HPSA) (SC50: 217.8-1238.39 µg/mL), 2,2'-azino-bis (3-etilbenzotiazolin-6-sülfonik asit) (ABTS) radikal giderme (SC50:20.15-27.14 µg/mL), 2, 2-difenil-1-pikrilhidrazil (DPPH) radikal giderme aktivitesi (SC50: 22.93-308.17 ug/mL) ve demir indirgeme gücü (FRAP) (% 71.03-99.54) ile değerlendirildi. Örneklerin karşılaştırılması için standart antioksidan bileşikler olarak butile hidroksi anizol (BHA), rutin (RUT) ve troloks (TRO) kullanıldı. Bu sonuçlara göre, örnekler ABTS analizi dışında TRO'dan daha verimli antioksidan aktivite göstermiştir. Ayrıca, Örnek 1 ve 4 daha yüksek FICA aktivitesine sahiptir ve Örnek 2 ve 3, standartlardan daha fazla FRAP aktivitesi göstermiştir. Bal örneklerinin Staphylococcus aureus ve Escherichia coli’ye karşı minimum inhibitör konsantrasyonları (MIC'ler) ve agar difüzyon deneyleri, bu numunelerin büyümelerini engellediğini gösterdi.

Anahtar Kelimeler

• Antimikrobiyal aktivite
• Antioksidant aktivite
• Apis mellifera
• Bal
• Fizikokimyasal analiz

Floral origin, Antioxidant and Antimicrobial Activity of Some Floral Honey

Öz

The study was planned to assess botanic origin, antioxidant power, antimicrobial activity and physicochemical properties of four honey samples were collected in Turkey. The melissopalynologic results revealed that sample 1 and 2 are polyfloral, and others are unifloral. Total content of phenolic (116.47-1711.13 mg GAE/100 g) and flavonoids (1.46 - 27.25 mg CAE/100 g) were analysed, and the antioxidant activities measured by ferrous ion chelating activity (FICA) (71.32-93.35%), hydrogen peroxide scavenging activity (HPSA) (SC50: 217.8-1238.39 µg/mL), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging (SC50: 20.15-27.14 µg/mL), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (SC50: 22.93-308.17 µg/mL) and ferric reducing antioxidant power (FRAP) (71.03-99.54 %) differed notably. For comparison of the samples, butylated hydroxy anisole (BHA), rutin (RUT) and trolox (TRO) were used as standard antioxidant compounds. According to these results, samples showed more efficient antioxidant activity than TRO except for ABTS assay. Also, Sample 1 and 4 have higher FICA activity and Sample 2 and 3 demonstrated more FRAP activity than standards. The minimal inhibitory concentrations (MICs) and agar diffusion assays of honey samples against Staphylococcus aureus and Escherichia coli showed that these samples was the most effective in inhibition of their growth.

Anahtar Kelimeler

• Antimicrobial activity
• Antioxidant activity
• Apis mellifera
• Honey
• Physicochemical analysis

Kaynakça

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