The apoptotıc effects of SCFAs from Lactobacillus reuteri on (HT-29) human colon cancer cells

Yıl 2017, Cilt: 6 Sayı: 2, 11 - 19, 16.12.2017

Ekrem Darendelioglu Mehmet Çiftci , Gıyasettin Baydas

Öz

Colon cancer is one
of the leading causes of death around the World. In colon or other types of
cancer, chemical methods can be considered as a therapeutic strategy. However,
probiotics can also be used as biotherapeutics to reduce recurrence and side
effects in patients with colon cancer.

Short Chain Fatty
Acids (SCFAs) the fermentation products of probiotic L. reuteri bacteria found in the gastrointestinal tract. There are
several SCFAs including acetic, propionic, butyric and lactic acids that have
been shown to have apoptosis inducing properties in human colon cancer cells.
In this study, the anti-proliferative and pro-apoptotic activities of
probiotics were explained and the effects of SCFAs produced by L. reuteri on mitochondrial apoptosis
were observed with changes in ROS and LPO production levels on human colon
cancer cells (HT-29).

Results suggested
that SCFAs from L. reuteri increased
the mortality rates of HT-29 cells, increased ROS and LPO production. In
addition to these effects, downregulation of Bcl-2, elevation of cytochrome c
and overproduction of caspase-3 protein in SCFAs-treated HT-29 cells were
observed. It was concluded that SCFAs from L.
reuteri have cytotoxic effects in HT-29 colon cancer cells.

Anahtar Kelimeler

Colon cancer, Probiotics, L. reuteri, HT-29, SCFAs, ROS, LPO

Lactobacillus reuteri’den elde edilen KZYA’nin insan kolon kanser hücrelerinde (HT-29) apoptotik etkileri

Öz

Kolon kanseri, dünyadaki
önde gelen ölüm nedenlerinden biridir. Kolon veya diğer kanser tiplerinde,
kimyasal yöntemler terapötik bir strateji olarak düşünülebilir. Bununla
birlikte, probiyotikler, kolon kanserli hastalarda hastalığın tekrarı ve yan
etkilerini azaltmak için biyoterapötikler olarak kullanılabilir.

Kısa Zincirli Yağ
Asitleri (KZYA), bağırsakta bulunan probiyotik L. reuteri bakterilerinin fermentasyon ürünleridir. İnsan kolon
kanseri hücrelerinde apoptoz indükleyici özelliklere sahip olduğu gösterilen asetik,
propiyonik, bütirik ve laktik asitleri içeren birçok KZYA vardır. Bu çalışmada,
probiyotiklerin anti-proliferatif ve pro-apoptotik aktiviteleri açıklanmış ve L. reuteri'nin ürettiği KZYA'nin, insan
kolon kanseri hücrelerinde (HT-29) mitokondriyal apoptoz yolağına etkileri ve
ROS ile LPO üretim düzeylerindeki değişimleri gözlemlenmiştir.

Sonuçlara göre, L. reuteri'den elde edilen KZYA, HT-29
hücrelerinde mortalite oranını, ROS ve LPO üretimini anlamlı şekilde
artırmıştır. Bu etkilere ilaveten KZYA ile tedavi edilen HT-29 hücrelerinde
Bcl-2 seviyesinin azalması, sitokromun c ve kaspaz-3 miktarlarının yükselmesi
gözlenmiştir. Tüm sonuçlar ışığında L.
reuteri kaynaklı KZYA'nin kolon kanseri hücrelerinde sitotoksik etkileri
olduğu sonucuna varılmıştır.

Anahtar Kelimeler

Kolon kanseri, Probiyotikler, L. reuteri, HT-29, KZYA, ROS, LPO

Kaynakça

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