EVOLUTIONARY-OBTAINED saccharomyces cerevisiae, RESISTANT TO DMEM CULTURED WITH PROSTATE CANCER CELLS

Yıl 2018, Cilt: 7 Sayı: 1, 11 - 17, 26.06.2018

Musa Tartık Gıyasettin Baydaş Mehmet Çiftçi

Öz



The complexity of the cell is a major problem to study on
cancer treatment. In order to find an accurate solution to cancer, it is
necessary to know the cell metabolism in detail, but the available knowledge
and technology are inadequate. Herein, there is a need for alternative
approaches to suggest a treatment way against cancer instead of trying to
understand the cell metabolism in details. Therefore, this study aimed to
obtain the desired phenotype of Saccharomyces
cerevisiae which can easily grow in the DMEM pre-cultured with PC3 (PCM).

Evolutionary engineering was successfully applied to wild
type (WT) yeast population to randomly generate a variety of genetic
phenotypes. The resistant mutants against PCM were randomly selected from an
agar plate. Furthermore, the best individual mutant being resistant in PCM was
determined with genetic stability tests.

In the current study, the resistant yeasts were obtained by
evolutionary engineering against PCM restricting the growth of WT. The growth
fitness of selected mutants dramatically increased in the PCM, when compared to
WT. The best mutant, MY2, is an example to be resistant in extreme environment
with directing the instinct of survival of organisms. To suggest as a cancer
treatment, the secondary metabolites of MY2 on cancer cells have to be tested.

Anahtar Kelimeler

Saccharomyces cerevisiae, cancer, evolutionary engineering, PC3, yeast

EVOLUTIONARY-OBTAINED saccharomyces cerevisiae, RESISTANT TO DMEM CULTURED WITH PROSTATE CANCER CELLS

Öz

Hücrenin
karmaşıklığı kanser tedavisi geliştirmek için büyük bir sorundur. Kansere karşı
doğru bir çözüm bulmak için, hücre metabolizmasını ayrıntılı olarak bilmek gerekir,
ancak mevcut bilgi ve teknoloji bunun için yetersizdir. Bu noktada, kanser
tedavisi için alternatif yaklaşımlara ihtiyaç duyulmaktadır. Bu nedenle, bu
çalışma, PC3 (PCM) ile önceden kültürlenmiş DMEM'de kolaylıkla büyüyebilen
mutant Saccharomyces cerevisiae fenotipinin elde edilmesini amaçlamıştır.

 

Evrimsel
mühendislik yöntemi başarılı bir şekilde uygulanarak, doğal tip maya (WT)
popülasyonunda çeşitli genetik fenotipler rasgele üretildi. PCM'ye karşı
dirençli mutantlar bir agar plakasından rastgele olacak şekilde seçildi.
Ayrıca, Seçilen mutant feneotipler içinden PCM'de dirençli en iyi bireysel
mutant genetik kararlılık testleri ile belirlendi.

 

Mevcut
çalışmada, WT'nin büyümesini sınırlayan PCM'ye karşı evrimsel mühendislik
yöntemiyle dirençli muatantlar elde edildi. Seçilen mutantların PCM içindeki
büyüme kapasitesi, WT'ye kıyasla anlamlı biçimde arttı. En iyi mutant olan MY2,
organizmaların hayatta kalma içgüdüsünü yönlendiren uç koşullarda dirençli
olmanın bir örneğidir. Kanser hücresi büyütülen ortamda rahatlıkla büyüye bilen
MY2, üretiği mikincil metabolitlerin kanser hücreleri üzerinde test edilmesi
gerekir. Elde edilen sonuçlara göre kanser tedavisinde kullanılabilirliği
değerlendirilmelidir.

Anahtar Kelimeler

saccharomyces cerevisiae, cancer, Evolutionary engineering, PC3, yeast

Kaynakça

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