Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi

H. Turan Akkoyun; A. Şükrü Bengü; Mahire Bayramoğlu Akkoyun; Aykut Ulucan; Mehmet Sait İzgi; Ömer Şahin; Suat Ekin; Şule Melek

doi:10.46810/tdfd.819741

Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi

Öz

Bu çalışmada, sıçanlarda civa II klorür'ün meydana getireceği beyin hasarının engellenmesinde borik asit (BA)'in koruyucu etkileri araştırıldı. Deneyde 200-300 gr ağırlığında, 24 adet yetişkin, Wistar albino cinsi erkek rat kullanıldı. Ratlar, Grup I (Kontrol,n:8):İzotonik serum uygulandı (i.p), Grup II (civa II klorür (HgCl2),n:8):Oral yol ile (0.01 g/kg), Grup III (HgCl2+BA,n:8): Oral yol ile HgCl2 (0.01 g/kg/gün) + BA (3.25mg/kg/gün) (i.p) konsantrasyonda uygulaması yapıldı. Tüm gruplardaki ratlar 10. günün sonunda sakrifiye edilerek beyin dokuları alındı. Biyokimyasal parametrelerden SOD, CAT ve GSH-Px antioksidan enzim aktiviteleri ölçüldü. Ayrıca histopatolojik olarak değerlendirildi. SOD enzim aktivitesi değerlendirildiğinde; Hg uygulanan grupta kontrole oranla azaldığı (p˂0.001) görüldü. BA, uygulanan grupta ise aktivitenin HgCl2 grubu ile kıyaslandığında arttığı (p˂0.001) tespit edildi. CAT enzim aktivitesi değerlendirildiğinde; kontrole oranla HgCl2 ve HgCl2+BA uygulanan gruplarda aktivitede azalma (p˂0.001) olduğu görüldü. HgCl2 uygulanan grupla karşılaştırıldığında BA uygulanan grupta enzim aktivitesinde artış belirlendi. GSH-Px enzim aktivitesi değerlendirildiğinde ise; kontrol grubu ile kıyaslandığında HgCl2 (p˂0.001) ve HgCl2+BA (p˂0.05) uygulanan gruplarda aktivitenin azaldığı görüldü. Ayrıca HgCl2 uygulanan grup ile karşılaştırıldığında BA uygulanan grupta artış olduğu görüldü (p˂0.001). SOD, CAT ve GSH-Px gibi antioksidan enzim aktivitelerinin anlamlı olarak değiştiği ve beyin dokusu histopatolojik incelemede BA'nın koruyucu etkisi olabileceği düşünülmektedir.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

H. Turan Akkoyun ^*
0000-0002-4547-8003
Türkiye

A. Şükrü Bengü
Türkiye

Mahire Bayramoğlu Akkoyun
0000-0001-5150-5402
Türkiye

Aykut Ulucan
0000-0001-8844-8237
Türkiye

Mehmet Sait İzgi
0000-0003-3685-3219
Türkiye

Ömer Şahin
0000-0003-4575-3762
Türkiye

Suat Ekin
0000-0002-6502-5028
Türkiye

Şule Melek
0000-0002-0677-722X
Türkiye

Yayımlanma Tarihi

30 Aralık 2020

Gönderilme Tarihi

2 Kasım 2020

Kabul Tarihi

14 Aralık 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 9 Sayı: 2

DOI

https://doi.org/10.46810/tdfd.819741

IZ

https://izlik.org/JA72SL85ML

Kaynak Göster

RIS / Bibtex

APA

Akkoyun, H. T., Bengü, A. Ş., Bayramoğlu Akkoyun, M., Ulucan, A., İzgi, M. S., Şahin, Ö., Ekin, S., & Melek, Ş. (2020). Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. Türk Doğa ve Fen Dergisi, 9(2), 127-133. https://doi.org/10.46810/tdfd.819741

AMA

1.Akkoyun HT, Bengü AŞ, Bayramoğlu Akkoyun M, vd. Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. TDFD. 2020;9(2):127-133. doi:10.46810/tdfd.819741

Chicago

Akkoyun, H. Turan, A. Şükrü Bengü, Mahire Bayramoğlu Akkoyun, vd. 2020. “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”. Türk Doğa ve Fen Dergisi 9 (2): 127-33. https://doi.org/10.46810/tdfd.819741.

EndNote

Akkoyun HT, Bengü AŞ, Bayramoğlu Akkoyun M, Ulucan A, İzgi MS, Şahin Ö, Ekin S, Melek Ş (01 Aralık 2020) Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. Türk Doğa ve Fen Dergisi 9 2 127–133.

IEEE

[1]H. T. Akkoyun vd., “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”, TDFD, c. 9, sy 2, ss. 127–133, Ara. 2020, doi: 10.46810/tdfd.819741.

ISNAD

Akkoyun, H. Turan - Bengü, A. Şükrü - Bayramoğlu Akkoyun, Mahire - Ulucan, Aykut - İzgi, Mehmet Sait - Şahin, Ömer - Ekin, Suat - Melek, Şule. “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”. Türk Doğa ve Fen Dergisi 9/2 (01 Aralık 2020): 127-133. https://doi.org/10.46810/tdfd.819741.

JAMA

1.Akkoyun HT, Bengü AŞ, Bayramoğlu Akkoyun M, Ulucan A, İzgi MS, Şahin Ö, Ekin S, Melek Ş. Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. TDFD. 2020;9:127–133.

MLA

Akkoyun, H. Turan, vd. “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”. Türk Doğa ve Fen Dergisi, c. 9, sy 2, Aralık 2020, ss. 127-33, doi:10.46810/tdfd.819741.

Vancouver

1.H. Turan Akkoyun, A. Şükrü Bengü, Mahire Bayramoğlu Akkoyun, Aykut Ulucan, Mehmet Sait İzgi, Ömer Şahin, Suat Ekin, Şule Melek. Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. TDFD. 01 Aralık 2020;9(2):127-33. doi:10.46810/tdfd.819741