Auxenochlorella protothecoides SC3'ten Ekstre Edilen Pigmentlerin Pseudomonas aeruginosa'ya karşı Antimikrobiyal Aktivitesinin Değerlendirilmesi

Öz

Günümüzde, doğal mikrobiyal pigmentler, antibiyotik direncinde kullanılabilecek potansiyel aday terapötik ajanlar olarak karşımıza çıkmaktadır. Bu çalışmada, yeşil alg pigmentlerinin antimikrobiyal aktivitesi gösterilmiştir. Yeşil alg SC3 izolatı Erzurum'da topraktan izole edildi. Metanol:dimetil sülfoksit ile ekstrakte edilen pigmentler kısmi olarak ince tabaka kromotografisi (TLC), UV-Absorbansı ve fourier-transform kızılötesi spektroskopi (FTIR) yöntemleri ile karakterize edildi. Pigmentlerin antimikrobiyal aktivitesi, Pseudomonas aeruginosa (ATCC 27853 ve klinik izolatı), Staphylococcus aureus (ATCC 25923) ve Escherichia coli (ATCC 25922)’ye karşı agar difüzyon ve mikroplak deneyleri ile belirlendi. SC3 izolatını tanımlamak için total DNA izole edildi ve hem 18S rRNA bölgesi hem de kloroplast genomu için 16S rRNA bölgesi evrensel primerler kullanılarak çoğaltıldı. Bu şekilde, SC3 izolatı Auxenochlorella protothecoides olarak tanımlandı ve Genbank'a kaydedildi (Erişim numarası: MW139225 ve MW063613). Çalışmamıza göre, en iyi antimikrobiyal aktivite P. aeruginosa'ya karşı olduğu belirlenmiştir. Sonuçlarımız, A. protothecoides yeşil alginden elde edilen total pigmentlerin antimikrobiyal aktivitesini ilk kez göstermektedir.

Anahtar Kelimeler

• Auxenochlorella protothecoides
• Antimikrobial
• Pigment.

Antimicrobial Activity of Pigments Extracted from Auxenochlorella protothecoides SC3 against Pseudomonas aeruginosa

Abstract

Nowadays, natural microbial pigments are emerging as potential candidate therapeutic agents that can be used in antibiotic resistance. In this paper, we report the antimicrobial activity of green algae pigments. Green algae SC3 isolate was isolated from soil in Erzurum. Pigments extracted by methanol: dimethyl sulfoxide solvents were partially characterized by Thin Layer Chromatography (TLC), UV-Absorbance, and Fourier-transform infrared spectroscopy (FTIR). The antimicrobial activity of pigments was determined with agar well diffusion and microbroth assays against Pseudomonas aeruginosa (ATCC 27853 and clinic isolate), Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). To identify green algae, total DNA was isolated and both 18S rRNA and 16S rRNA for the chloroplast genome were amplified using universal primers. Thus, this alga was identified as Auxenochlorella protothecoides and submitted to Genbank (Accession number: MW139225 and MW063613). According to our results, the best antimicrobial activity was recorded against P. aeruginosa. Our results show for the first time the antimicrobial activity of the total pigments from A. protothecoides green algae.

Keywords

• Auxenochlorella protothecoides
• Antimicrobial
• Pigment

Kaynakça

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