SSR Markörleri Kullanılarak Bazı Upland Pamuk (Gossypium hirsutum L.) Genotiplerinin Genetik Çeşitlilik Analizlerinin Yapılması

Öz

Pamuk bitkisi dünya genelinde biyotik ve abiyotik stres koşullarında yetiştirilen çok önemli bir tarla bitkisidir. Bu stres koşullarıyla mücadelede kimyasal ilaçlarının olumsuz etkilerinden kaçınmanın en etkili yolu, bitki ıslah metodlarıyla biyotik ve abiyotik stres faktörlerine dayanıklı/tolerant yeni bitki çeşitlerini geliştirmektir. Bu çalışmamızda bazı upland pamuk genotipleri 20 adet polimorfik SSR primerleriyle taranmış, populasyon yapıları ve genetik çeşitlilik analizleri yapılmıştır. 17 SSR primeri lokus başına 5.82 allel olmak üzere totalde 99 allel üretmiştir. Markörlerin ortalama PIC değerleri 0.312 olmuştur. En yüksek PIC değeri (0.491)’ni Nau3736 SSR marköründe elde edilirken, en düşük PIC değeri (0.105) Bnl1611, Bnl3449 markörlerinde elde edilmiştir. Markörlerin genetik mesafe değerleri 0.26ile 1.09arasında değişmiştir. En yüksek genetik mesafe Sure Grow 96 ve Carmen, Sealand-542 ve Siokra ¼ ile Sphinx V ve Stoneville-453 arasında ölçülmüştür. Sonuç olarak, genetik olarak birbirinden uzak olan çeşitler (Acala maxxa, Carmen, Aleppo 40, Siokra ¼, and Tex), stres faktörlerine karşı dirençli çeşit geliştirme teknolojisi olan Markör Destekli Seleksiyon (MAS) ıslah programına ebeveyn olarak kullanılabileceği güçlü bir şekilde önerilmektedir.

Anahtar Kelimeler

• Genetik çeşitlilik
• MAS
• PIC
• SSR
• Upland pamuk

Genetic Diversity Analysis of Some Upland Cotton (Gossypium hirsutumL.) Genotypes Using SSR Markers

Abstract

Cotton plant is an important crop cultivated under biotic and abiotic stress conditions worldwide. The best way to avoid the harmful effects of chemicals used to combat these stresses is to develop tolerant or resistant varieties in plant breeding programs. In the present study, some of Upland cotton varietieswerescreened with 20 polymorphic SSR primers, and their population structure and genetic diversity analysis were examined. 17 SSR primers amplified 99 alleles with a 5.82 allele per locus. The mean PIC value of the markers was 0.312. The highest PIC value (0.491) belongs to the Nau3736 SSR marker while Bnl1611 and Bnl3449 markers had the lowest PIC value (0.105). The Genetic Distance (GD) values of the markers varied between 0.26and 1.09. The highest GD values were between Sure Grow 96 and Carmen, Sealand-542 and Siokra ¼, and between Sphinx V and Stoneville-453 cultivars. As a result, the genetically distantcultivars (Acala maxxa, Carmen, Aleppo 40, Siokra ¼, and Tex) can be recommended to use as parents in Marker-assisted selection (MAS) technology to develop new cotton varieties which are resistant or tolerant to stress factors.

Keywords

• Genetic diversity
• MAS
• PIC
• SSR
• Upland cotton

Kaynakça

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