A Field Study on Determination of Nematode Diversity in Canola Fields in Tekirdağ, Turkey

Abstract

Soils collected from 28 canola fields in Malkara, Süleymanpaşa, Muratlı, Hayrabolu, and Ergene districts of Tekirdağ were examined for the determination of nematode fauna. In the soils taken from 0-60 cm depth in each field, 28 genera of nematodes were recovered by the modified Baermann Funnel method. Of these, eight were bacterivores, three were fungivores, five were omnivores, three were predators, and the rest were plant-parasitic species. Nematodes in survey areas had colonizer-persister values ranging from 1 to 5. The maturity and community indices were also calculated and four were found as follows: Enrichment (E): 34.6±15.2; Maturity (MI): 2.68±0.05; Plant parasite (PPI): 2.69±0.39; Shannon's diversity (H'): 1.80±0.26. The predominant taxa were Filenchus (100%), Mesodorylaimus (92%), and Ditylenchus (85.1%). In terms of density in 100 cm3 soil, the highest values were found in the genus Acrobeloides (192 individuals/100 cm3 soil), and the number of Ditylenchus (57 individuals/100 cm3 soil), Filenchus (180 individuals/100 cm3 soil), and Mesodorylaimus (165 individuals/100 cm3 soil), individuals were also found high in some soils. The plant parasitic nematodes identified in canola fields include Boleodorus tylactus, Filenchus cylindricus, Filenchus sheri, Filenchus thornei, Geocenamus brevidens, Helicotylenchus digonicus, Malenchus fusiformis, Paratylenchus variabilis, Pratylenchoides alkani, Rotylenchus robustus, Tylenchorhynchus annulatus, and Tylenchorhynchus cylindricus.

Keywords

• Nematode diversity
• canola
• Tekirdağ
• Turkey

Kaynakça

1. AAFC. Agriculture and Agri-Food Canada – Regulatory Directive DIR 94-09: The Biology of Brassica napus L. (Canola/ Rapeseed). 1994;11.
2. Allen MW. A review of the nematode genus Tylenchorhynchus. Uni Calif Pub Zool. 1955;61:129-166.
3. Andrássy I. Revision der Gattung Tylenchus Bastian, 1865 (Tylenchidae, Nematoda). Acta Zool. Hung. 1954;1:5-42.
4. Anonymous. Turkish Statistical Institute; 2021. Available from: https://www.tuik.gov.tr.
5. Baadl S, Mahdikhani Moghadam E, Rouhani H. Identification of Aphelenchoides Fischer, 1849 species (Nematoda: Aphelenchoididae) collected from rapeseed fields in North Khorasan province. Journal of Iranian Plant Protection Research. 2016;29(4):558-570. doi: 10.22067/jpp.v29i4.32940.
6. Bongers R. The maturity index: An ecological measure of environmental disturbance based on nematode species composition. Oecologia. 1990;83:14-19.
7. De Man JG. Onderzoekingen over vrij in de aarde levende Nematoden. Tijdschrift Nederlandsche voor de Dierkunde Vereen. 1876;2:78-196.
8. Elliot CA. Diversity indices. – In Hunter MLJr Wildlife, forests, and forestry. Prentice Hall. Engelwood Cliffs, N.J. 1990; 297-302.

9. Fatemy S, Abootorabi E, Ebrahimi N, Aghabeigi F. First report of Pratylenchus neglectus and P. thornei infecting canola and weeds in Iran. Plant Dis. 2006;90(12):1555. doi: 10.1094/PD-90-1555B. PMID: 30780991.
10. Jenkins W RA. Rapid centrifugal-flotation technique for separating nematodes from soil. Plant Dis Rep.1964;48:692.
11. Jing GN, Halbrendt MG. Nematicidal compounds from rapeseed (Brassica napus and B. campestris). Journal of the Pennsylvania Academy of Science. 1994;68(1):29-33. JSTOR, http://www.jstor.org/stable/44148985.
12. Kepenekci İ. Plant parasitic nematodes (Tylenchida, Nematoda) in Turkey. Pakistan Journal of Nematology. 2014; 32 (1): 11-31.
13. Khan M, Khan S. Two new and a known species of Tylenchus Bastian (Nematoda: Tylenchinae) from Afghanistan. Nematol. Mediterr. 1978;6:213-221.
14. Manachini B, Landi S, Tomasini V. Biodiversity of nematofauna of oilseed rape (Brassica napus L.). Commun Agric Appl Biol Sci. 2005;70(4):927-35. PMID: 16628940.
15. Munawar M., Yevtushenko DP., Castillo P. Overview of the genus Boleodorus and first reports of Boleodorus thylactus and B. volutus from Southern Alberta, Canada. Animals 2021; 11:1760. https://doi.org/10.3390/ ani11061760
16. Munawar M., Castillo P., Yevtushenko DP. Description of Filenchus species from agroecosystem of Southern Alberta, Canada. Agronomy. 2022; 12(3):690. https://doi.org/10.3390/agronomy12030690
17. Okada H., Harada, H., Kadota I. Fungal-feeding habits of six nematode isolates in the genus Filenchus. Soil Biol. Biochem. 2005; 37: 1113–1120.
18. Okada H., Kadota I. Host status of 10 fungal isolates for two nematode species, Filenchus misellus and Aphelenchus avenae. Soil Biol. Biochem. 2003; 35: 1601–1607
19. Owen KJ, Clewett TG, Thompson JP. Pre-cropping with canola decreased Pratylenchus thornei populations, arbuscular mycorrhizal fungi, and yield of wheat. Crop and Pasture Science. 2010;61:399-410. https://doi.org/10.1071/CP09345.
20. Potter, J. W. and McKeown, A. W. 2003. Nematode biodiversity in Canadian agricultural soils. Can. J. Soil Sci. 83: 289–302.
21. Rosmaninho T, Mota M, Inácio ML, Eisenback JD, Gutiérrez-Gutiérrez C. Six first reports of pin nematodes from Portugal, with an update of the systematics, genetic diversity, and phylogeny of the genus Paratylenchus (Nematoda: Tylenchulidae). Horticulturae. 2022;8:343. https://doi.org/10.3390/ horticulturae8040343.
22. Seinhorst JW. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica. 1959;4(1):67-69. https://doi.org/10.1163/187529259X0038.
23. Shannon CE. A mathematical theory of communication. The Bell System Technical Journal. 1948;27:379-423. https://doi.org/10.1002/j.1538-7305.1948.tb01338.x.
24. Siddiqi MR. Tylenchida: parasites of plants and insects. 2nd edition. Wallingford Oxon: CAB International; 2000:833 pp.
25. Simon ACM, Lopez-Nicora HD, Ralston TI, Richer EA, Niblack TL, Paul PA. First report of stunt nematode (Tylenchorhynchus annulatus) on corn in Ohio. Plant disease.2020;104:2527. doi: 10.1094/PDIS-02-20-0429-PDN.
26. Simpson EH. Measurement of diversity. Nature. 1949;163:688. http://dx.doi.org/10.1038/163688a0.
27. Süzer S. Kanola Tarımı. Edirne, 2001;Yayın No:77-78.
28. Taher, I. E., Ami, S. N., Haleem, R. A., & Shareef, B. (2017). First record of mycetophagous nematode Aphelenchus avenae In Iraq with description and testing their propagatıon on different fungus culture. Bulletin of The Iraq Natural History Museum, 14, 251-259. 10.26842/binhm.7.2017.14.3.0251.
29. Taylor S, Szot D. First record of damage to canola caused by the oat race of stem nematode (Ditylenchus dipsaci). Australasian Plant Pathology. 2000;29:153. https://doi.org/10.1071/AP00027.
30. Thorne G. Some nematodes of the family Tylenchidae which do not possess a valvular median esophageal bulb. Great Basin Naturalist. 1941;2(2):1.
31. Thorne G, Malek RB. Nematodes of the northern Great Plains. Part I. Tylenchida (Nemata: Secernentea). Tech. Bull. S. Dak. Agric. Exp. Stn. 1968;1:72-76.
32. Tıraş M. Türkiye’de kanola tarımı. Doğu Coğrafya Dergisi. 2011;14(21):159-172. Retrieved from https://dergipark.org.tr/tr/pub/ataunidcd/issue/2436/31181.
33. Yeates GW, Bongers T, de Goede RGM, Freckman DW, Georgieva SS. Feeding habits in soil nematode families and genera-an outline for soil ecologists. Journal of Nematology. 1993;25:315-331.
34. Yilmaz H, Avkiran B. Analysis of canola (rapeseed) production cost and income in context of oilseeds production support policies: A case study from Trakya region of Turkey. Ekonomika poljoprivrede. 2020;67:483-493. 10.5937/ekoPolj2002483Y.
35. Yuen PH. Four new species of Helicotylenchus Steiner (Hoplolaiminae: Tylenchida) and a redescription of H. canadensis Waseem, 1961. Nematologica.1964;10(3):373-387.
36. Yüksel HŞ. Pratylenchoides alkani n. sp. and P. erzurumensis n. sp. (Nematoda: Tylenchoidea) from soil in Turkey. Proceedings of the Helminthological Society of Washington.1977;44:185-188.
37. Wouts WM, Sturhan D. Descriptions of Rotylenchus species from New Zealand, including R. labiodiscus sp. n. (Nematoda: Hoplolaimidae), New Zealand Journal of Zoology. 1999;26(4):395-404. doi: 10.1080/03014223.1999.9518202.