Drosophila melanogaster Modelinde Maleik Asit ve Vanilik Asitin Bakır Toksisitesi Üzerine Etkilerinin Araştırılması

Öz

Bakır, tüm canlı organizmaların biyolojik fonksiyonlarının sürdürülmesi için gerekli olan bir metaldir. Bakır, düşük konsantrasyonlarda hücresel metabolizmanın sürdürülmesi için gerekli olmasına rağmen, yüksek konsantrasyonlarda ROS oluşumuna neden olduğundan toksik etkilere neden olabilir. Bu çalışmada larva ve yetişkin D. melanogaster'de CuSO4 (1 mM) ile toksisite oluşturuldu. Sinekler daha sonra maleik asit (MA) (2 mg) ve vanilik asit (VA) (2 mg) ile işlendi. Sonuçlar Cu toksisitesinin SOD, CAT, GPX, AChE ve GSH düzeylerinde azalmaya neden olduğunu gösterdi. MDA düzeylerinde önemli bir artış oldu. Ancak MA ve VA tedavisinin SOD, CAT, GPX, AChE ve GSH miktarlarını artırdığı, MDA miktarını ise azalttığı belirlendi. Bu sonuçlar MA ve VA'nın CuSO4'ün neden olduğu ROS ve oksidatif stres üzerinde iyileştirici etkilere sahip olduğunu gösterdi. Sonuç olarak, gelecekteki çalışmalarda metal kaynaklı toksisiteye karşı doğal bileşiklerin farklı biyolojik parametreler üzerindeki etkileri değerlendirilmelidir.

Anahtar Kelimeler

• Antioksidan
• Drosophila
• Maleik asit
• Vanilik asit

Investigation of the Effects of Maleic Acid and Vanillic Acid on Copper Toxicity in the Drosophila melanogaster Model

Abstract

Copper is a metal that is necessary for the maintenance of biological functions of all living organisms. Although copper is essential for the maintenance of cellular metabolism at low concentrations, at high concentrations it can cause toxic effects as it causes ROS formation. In this study, toxicity was induced by CuSO4 (1 mM) in larval and adult D. melanogaster. The flies were then treated with maleic acid (MA) (2 mg) and vanillic acid (VA) (2 mg). The results showed that Cu toxicity caused a decrease in SOD, CAT, GPX, AChE and GSH levels. There was a significant increase in MDA levels. However, it was found that treatment with MA and VA increased the amounts of SOD, CAT, GPX, AChE and GSH and decreased the amount of MDA. These results showed that MA and VA had ameliorative effects on ROS and oxidative stress caused by CuSO4. In conclusion, the effects of natural compounds on different biological parameters against metal-induced toxicity should be evaluated in future studies.

Keywords

• Antioxidant
• Drosophila
• Maleic acid
• Vanillic acid

Kaynakça

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