Determination of Antioxidant-Antiradical Activity and Vitamin C Content of Peucedanum longifolium Waldst. & Kit

Abstract

In the presented study, the aim was to investigate the antioxidant and antiradical properties, as well as the vitamin C content, of Peucedanum longifolium Waldst. & Kit. The total phenolic, flavonoid contents and antioxidant capacity, DPPH radical scavenging capacity, and superoxide radical elimination ability of Peucedanum longifolium were evaluated spectrophotometrically. Additionally, the vitamin C content—an important antioxidant vitamin—was determined using the dinitrophenylhydrazine (DNPH) method. The phenolic and flavonoid contents and total antioxidant capacity of P. longifolium were found to be 4.98±0.06 mg gallic acid/g, 4.92±0.21 mg quercetin/g, and 26.46±2.83 mM ascorbic acid/g, respectively. The vitamin C content was determined to be 113.18±2.36 mg/100 g. The highest inhibition percentages for the DPPH and ABTS radicals were measured as 85.58±2.12% and 78.08±0.38%, respectively. The plant exhibited superior superoxide and hydroxyl radical scavenging capacities and metal chelating activity compared to the positive controls. However, its hydrogen peroxide scavenging activity was found to be weaker than that of the positive control, BHT. In conclusion, the high vitamin C content and strong antioxidant and free radical scavenging activities of Peucedanum longifolium suggest that this species may have protective potential against diseases caused by free radicals. The findings not only provide a scientific foundation for the informed use of the plant in traditional or complementary medicine, but also serve as a valuable reference for future research.

Keywords

• Peucedanum longifolium Waldst.&Kit
• DPPH
• ABTS
• superoxide
• hydroxyl
• radical
• vitamin C

Peucedanum longifolium Waldst. & Kit'in Antioksidan-Antiradikal Aktivitesi ve C Vitamini İçeriğinin Belirlenmesi

Öz

Sunulan çalışmada; Peucedanum longifolium Waldst. & Kit bitkisinin antioksidan/ antiradikal özellikleri ve C vitamini içeriğinin araştırılması amaçlandı. Peucedanum longifolium Waldst. & Kit fenol, flavanoid, total antioksidan içeriği, DPPH, ABTS, hidroksil, superoksit radikali süpürme kapasitesi ve bitkinin metal şelatlama aktivitesi spektrofotometrik olarak değerlendirildi. Ayrıca önemli antioksidan bir vitamin olan C vitamini içeriği ise dinitrofenilhidrazin metodu kullanılarak belirlendi. P.longifolium Waldst.&Kit bitkisinin fenol ve flavanoid, total antioksidan içerikleri sırasıyla 4,98±0,06(mg gallik asit/g), 4,92±0,21(mg kuersetin/g) 26,46±2,83(mM askorbik asit/g) olarak Vitamin C içeriği ise 113,18±2,36 (mg/100g) olarak bulundu. Bitkinin DPPH radikali ve ABTS radikalleri en yüksek inhibisyon yüzdelerinin sırasıyla %85,58±2.12 ve %78,08±0,38 olduğu belirlendi. Bitkinin Süperoksit ve hidroksil radikali giderme kapasitelerinin ve metal şelatlama aktivitesinin pozitif kontrollere oranla daha iyi düzeyde olduğu görüldü. Hidrojen peroksit giderme aktivitesinin ise pozitif kontrol olarak kullanılan BHT’ye oranla zayıf olduğu belirendi. Sonuç olarak, Peucedanum longifolium bitkisinin yüksek düzeyde C vitamini içermesi, güçlü antioksidan ve serbest radikal süpürme aktivitelerine sahip olması, bu türün serbest radikallerin neden olduğu hastalıklara karşı koruyucu potansiyele sahip olabileceğini göstermektedir. Elde edilen verilerin, bitkinin geleneksel ya da tamamlayıcı tıpta bilinçli kullanımı için bilimsel bir temel oluşturmasının yanı sıra, ileride yapılacak çalışmalara da referans olacağı düşünülmektedir.

Anahtar Kelimeler

• Peucedanum longifolium Waldst.&Kit
• DPPH
• ABTS
• süperoksit
• hidroksil
• radikal
• C vitamini

Kaynakça

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