Yaşlanma ve Yüzme Egzersizinin, Torasik Aorta ve Gastroknemius İletim Arterlerinde Karbon Monoksit Gevşeme Yanıtına Etkisi

Öz

Amaç: Bu çalışmanın amacı yaşlanma ve yüzme egzersizine bağlı olarak sıçanların torasik aortasında ve gastroknemius iletim arterinde karbon monoksit CO ’e verilen damar yanıtlarının değişip değişmediğini test etmektir.Yöntem: Çalışmamızda 4 aylık genç ve 24 aylık yaşlı Wistar dişi sıçanlar kullanıldı. Sıçanlar rastgele gençsedanter GS , genç-egzersiz GE , yaşlı-sedanter YS ve yaşlı-egzersiz YE olmak üzere dört gruba ayrıldı. Egzersiz gruplarına 8 hafta süresince haftada 5 gün, günde 1 saat yüzme egzersizi yaptırıldı. Sıçanlardan izole edilen torasik aorta halkaları organ banyosu düzeneğinde, gastroknemius iletim arteri ise telli miyograf düzeneğinde çalışıldı. Aort ve gastroknemius iletim arteri halkalarının fenilefrin PE kasılma yanıtları hemoksijenaz inhibitörü chromium mesoporphyrin inkübasyonu öncesi ve sonrasında kaydedilerek endojen olarak üretilen CO’in vasküler tonusa katkısı değerlendirildi. CO donörü CORM; carbon - monoxide - releasing - molecule kullanılarak ekzojen gevşeme yanıtları değerlendirildi.Bulgular: CrMP inkübasyonu öncesinde ve sonrasında PE doz yanıt eğrilerinde hem aortada hem de gastroknemius iletim arterinde gruplar arasında fark p

Anahtar Kelimeler

• Karbon monoksit, hemoksijenaz, vasküler tonus, aorta, gastroknemius iletim arteri

The Effect of Aging and Exercise Training on Carbon Monoxide Relaxation Response in Thoracic Aorta and Gastrocnemius Feed Artery

Abstract

Objective: The purpose of this study is to observe any aging and swimming exercise associated changes in the rat thoracic aorta and gastrocinemius feed artery.Methods: 4-month and 24-month old female rats were used and divided into four following groups: sedentary young, trained young, sedentary old, and trained old. Swimming exercise was performed for 8 weeks 60 min/day, 5 days/week . Thoracic Aorta and gastrocinemius feed artery isolated from the rats. Thoracic Aorta rings were mounted on organ bath and gastrocinemius feed arteries were mounted on wire myograph. Contraction responses of all vessel rings in presence and absence of HO inhibitor CrMP were recorded as an endogenous CO contribution to vascular tonus. The effect of exogenous CO relaxation response were assesed by CO releasing molecule CORM .Results: Phenylephrine dose- response curves with or without CrMP were smilar in all groups both aortic rings and gastrocnemius feed artery. The maximum PE contraction responses obtained after CrMP incubation were significantly higher in the GE, YS and YE groups Endotel disfonksiyonu, sadece esansiyel hipertansiyon veya sekonder hipertansiyon 1, 2 , hiperkolesterolemi 3, 4 ve ateroskleroz 4 gibi hastalıkların karekteristik özelliği olmayıp günümüzde yaşlılıkla da ilişkilendirilmektedir 5, 6 . Bu patolojik durumlardan bağımsız olarak da yaşlılığın, torasik aorta ve direnç arterlerinde endotel disfonksiyonuna yol açtığı rapor edilmiştir 5, 7, 8 . Endotel fonksiyonunda yaşlanmayla birlikte görülen değişikliklerin kardiyovasküler hastalıkların patogenezinde klinik açıdan önemli etkileri olabilir. Yaşa bağlı olarak görülen endotel fonksiyonlarındaki bozulma / azalma öncelikle endotel hücrelerinin vasküler tonusu düzenlemek için salgıladıkları gevşetici mediatörlerin azalması ile kendini gösterir. Bu durum endotelden salgılanan çeşitli vazodilatör maddelerin ve özellikle nitrik oksidin NO hem üretiminin hem de bioyararlanımının azalması ile açıklanmaktadır 9 . Bunun yanında yaşlanma p

Keywords

• Carbon monoxide, hemo oxygenase, vascular tonus, aorta, gastrocnemius feed artery

Kaynakça

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