Effects on Growing of Scenedesmus spp. use of Different Medium

Yıl 2023, Cilt: 1 Sayı: 2, 7 - 16, 31.12.2023

Selin Doğan , Zeliha Akyüz , Rabia Tatar , Emirhan Ögrü , Muhammet Furkan Topal , Yusuf Gülşahin , Gökçe Karadayı , Mehmet Karadayı , Özden Fakıoğlu , Medine Güllüce

Öz

The aim of this study is investigated in the effects of different medium on growing performans Scenedesmus spp., which is an industrially important member of the Chlorophyceae (Green algae) group. For this purpose, Scenedesmus spp. is isolated from natural lakes within the borders of Erzurum province by from samples taken with a plankton net. Pure cell culture growth of Scedesmus spp. in BBM (Bold's Basal Medium), 3N-BBM (3 Nitrogen Bold's Basal Medium), WCM (Wright's Cryptophyte Medium), BG-11 (Blue Green Algal) was monitored during 14 days in incubation. At the end of the try has been found the highest growth in 3N-BBM culture medium with a mean cell counting of 355 cells/ml, and the lowest growth in WCM culture medium with a mean cell counting of 86 cells/ml. As a result of in this try has been determined that the culture medium enriched with nitrogen (N) is effective positive impact on growing of Scenedesmus spp.

Anahtar Kelimeler

BBM, 3N-BBM, WCM, BG-11 Medium, Scenedesmus spp.

Proje Numarası

TÜBİTAK ARDEB-1001 (122O2973)

Farklı Kültür Ortamlarının Scenedesmus spp. Gelişimi Üzerine Etkileri

Öz

Bu çalışmanın amacı Chlorophycea (Yeşil algler) grubunun endüstriyel açıdan önemli bir üyesi olan Scenedesmus spp. türlerinin gelişimleri üzerine farklı kültür ortamlarının etkilerinin araştırılmasıdır. Bu amaçla Erzurum il sınırları içerisinde yer alan doğal göllerden plankton kepçesi ile alınan örneklerden Scenedesmus spp. izole edilmiştir. Scedesmus spp.’nin BBM (Bold’s Basal Medium), 3N-BBM (3 Nitrogen Bold’s Basal Medium), WCM (Wright’s Cryptophyte Medium), BG-11 (Blue Green Algal) saf hücre kültür büyümeleri 14 günlük inkübasyon süresince takip edilmiştir. Deneme sonunda en yüksek gelişim ortalama 355 x 104 adet/ml hücre sayısıyla 3N-BBM kültür ortamında, en düşük gelişim ortalama 86 x 104 adet/ml hücre sayısıyla WCM kültür ortamında hesaplanmıştır. Sonuç olarak azot (N) ile zenginleştirilmiş kültür ortamının Scedesmus spp. gelişimine pozitif etki ettiği tespit edilmiştir.

Anahtar Kelimeler

BBM, 3N-BBM, WCM, BG-11 Medium, Scenedesmus spp.

Proje Numarası

TÜBİTAK ARDEB-1001 (122O2973)

Kaynakça

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