Paraoxonase 1 gene polymorphısms (Q192r and L55m) and assocıatıon wıth coronary slow flow

Öz

Aim: Coronary slow flow (CSF) is an angiographic entity characterized by slow progression of opaque material and an early indicator of atherosclerosis. Paraoxonase 1 (PON1) protects high-density lipoprotein (HDL) and low-density lipoprotein (LDL) from oxidative modifications. PON 1 has two amino acid polymorphisms (192Q/R and 55L/M) and that affect its functioning. We aim to determine PON1 two genetic polymorphisms and relationship with CSF. As we know, our study is the first to assessment the relationship PON1 gene polymorphisms (L55M and Q192R) and CSF. Materials and Methods: We included a total of 100 patients and 2 groups as normal coronary flow (NCF) and CSF. Genomic sequences of rs854560 and rs662 polymorphisms were determined using polymerase chain reaction. The research protocol was approved by Fırat University Institutional Review Board (Approval No:16). Results: The mean age of CSF group was 45.4±17 and NCF group was 50.5±11 years. There was the statistically difference in terms of the frequency of carrying Q and R alleles. For dual genotypes, the QQLM genotype was more common in CSF group, whereas the QRLM genotype was more common in NCF. Significant differences were found between patients with QQLM, RRLL, RRLM and QRLM genotypes and healthy individuals. Conslusion: We found a significant relationship between the Q allele and the QQLM genotype and CSF, and we thought that these may be risk factors for CSF. In addition, the fact that the R allele and QRLM, RRLL, and RRLM genotypes were higher in the NCF group and there was a statistically significant relationship suggested that these might be protective factors for CSF.

Anahtar Kelimeler

• coronary slow flow
• paraoxonase
• gene polymorphism
• antioxidant

Paraoksonaz 1 gen polimorfizmleri (Q192r ve L55m) ve koroner yavaş akımla ilişkisi

Öz

Amaç: Koroner yavaş akım (KYA), opak maddenin yavaş ilerlemesi ile karakterize anjiyografik bir antitedir ve aynı zamanda ve aterosklerozun erken bir göstergesi olduğu düşünülmektedir. İnsan paraoksonazı 1 (PON1), yüksek yoğunluklu lipoproteini (HDL) ve düşük yoğunluklu lipoproteini (LDL) oksidatif modifikasyonlardan korur. Paraoksonaz 1'in işleyişini etkileyen iki amino asit polimorfizmi (192Q/R ve 55L/M) vardır. PON1 iki genetik polimorfizmi ve koroner yavaş akım ile ilişkisini belirlemeyi amaçlıyoruz. Bildiğimiz kadarıyla çalışmamız PON1 gen polimorfizmleri (L55M ve Q192R) ile KYA ilişkisini değerlendiren ilk çalışmadır. Gereç ve Yöntemler: Çalışmaya toplam 100 hasta dahil edildi ve normal koroner akım (NKA) ve KYA olmak üzere 2 gruba ayrıldı. rs854560 ve rs662 polimorfizmlerinin genomik dizileri polimeraz zincir reaksiyonu kullanılarak belirlendi. Araştırma protokolü Fırat Üniversitesi Kurumsal İnceleme Kurulu tarafından onaylanmıştır (Onay No:16). Bulgular: Koroner yavaş akım grubunun yaş ortalaması 45,4±17 ve NKA grubunun 50,5±11 idi. Q ve R alellerini taşıma sıklığı açısından istatistiksel olarak fark vardı. Dual genotipler için, QQLM genotipi KYA grubunda daha yaygınken, QRLM genotipi NKA'de daha yaygındı. QQLM, RRLL, RRLM ve QRLM genotiplerine sahip hastalar ile sağlıklı bireyler arasında anlamlı fark bulundu. Sonuçlar: Q aleli ile QQLM genotipi ve KYA arasında anlamlı bir ilişki bulduk ve bunların KYA için risk faktörleri olabileceğini düşündük. Ayrıca R aleli ile QRLM, RRLL ve RRLM genotiplerinin NKA grubunda daha yüksek olması ve istatistiksel olarak anlamlı bir ilişki bulunması bunların KYA için koruyucu faktörler olabileceğini düşündürdü.

Anahtar Kelimeler

• koroner yavaş akım
• paraoksonaz
• gen polimorfizmi
• antioksidan

Kaynakça

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