Kronik hepatit B hastalarında HBV-DNA düzeylerinin biyokimyasal ve mikrobiyolojik belirteçlerle ilişkisi

Öz

Amaç: HBV-DNA seviyeleri, Kronik hepatit B (KHB) hastalığını teşhis etmek, enfeksiyon evresini belirlemek, tedaviye karar vermek ve hastalığın seyrini belirlemek için kullanılır. HBeAg aktif viral replikasyonun ve transkripsiyonun, Alanin aminotransferaz (ALT) ve Aspartat aminotransferaz (AST) karaciğer inflamasyonunun bir belirtecidir. Bu çalışma ile hastanemizde KHB ön tanısıyla takip edilen hastaların HBV-DNA düzeylerinin yaş, biyokimyasal ve mikrobiyolojik parametreler arasındaki ilişkinin araştırılması amaçlanmaktadır. Gereç ve Yöntemler: Mayıs 2021- Mayıs 2024 tarihleri arasında hastanemizde KHB ön tanısı ile takip edilen 264 hastanın kan örneklerinden HBV-DNA, mikrobiyolojik ve biyokimyasal parametrelere ait test sonuçları retrospektif olarak incelenmiştir. HBV-DNA düzeyleri HBV-DNA Negatif (Grup 1), HBV-DNA 10-2000 IU/mL (Grup 2) ve HBV-DNA>2000 IU/mL (Grup 3) olmak üzere üç gruba ayrılmıştır. İstatistiksel analizler MedCalc (version 20.009; Ostend, Belgium) istatistik paket programı ile yapılmıştır. Bulgular: HBV-DNA negatif hastalarda HBV-DNA 10-2000 IU/mL ve HBV-DNA >2000 IU/mL olan hastalara göre, HBV-DNA 10-2000 IU/mL olan hastalarda da HBV-DNA>2000 IU/mL olan hastalara göre HBeAg pozitifliği anlamlı derecede düşük tespit edilmiştir (p<0.05). ALT ve AST değerleri ise HBV-DNA>2000 IU/mL olan hastalarda HBV-DNA negatif ve HBV-DNA 10-2000 IU/mL olan hastalara göre anlamlı derecede yüksek saptanmıştır. HBV-DNA düzeyleri ile WBC, HGB, MCV, RDW, GGT, ALP, Total protein, albümin, PT, aPTT, INR değerleri arasında istatistiksel olarak anlamlı bir ilişki bulunmamıştır. Sonuçlar: KHB hastalarında HBeAg ve ALT, AST değerleri ile HBV-DNA düzeyleri arasında anlamlı bir ilişki saptanmıştır. Bu parametreler birlikte KHB hastalığını teşhis edip enfeksiyon evresini oluşturmak, tedaviye karar vermek ve hastalığın seyrini belirlemek için kullanılabilir.

Anahtar Kelimeler

• HBV-DNA
• HBeAg
• ALT
• Kronik hepatit B

Relationship of HBV-DNA levels with biochemical and microbiological markers in chronic hepatitis B patients

Öz

Objective: HBV-DNA levels are used to diagnose Chronic Hepatitis B (CHB), determine the stage of infection, decide on treatment and determine the course of the disease. HBeAg is a marker of active viral replication and transcription, Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) are markers of liver inflammation. This study aims to investigate the relationship between HBV-DNA levels and age, biochemical and microbiological parameters in patients followed up with a preliminary diagnosis of CHB in our hospital. Materials and Methods: HBV-DNA, microbiological and biochemical parameters test results from blood samples of 264 patients followed up in our hospital with a preliminary diagnosis of CHB between May 2021 and May 2024 were retrospectively analyzed. HBV-DNA levels were divided into three groups as HBV-DNA Negative (Group 1), HBV-DNA 10-2000 IU/mL (Group 2) and HBV-DNA>2000 IU/mL (Group 3). Statistical analyses were performed with the MedCalc (version 20.009; Ostend, Belgium) statistical package program. Results: HBeAg positivity was significantly lower in HBV-DNA negative patients compared to patients with HBV-DNA 10-2000 IU/mL and HBV-DNA >2000 IU/mL and in patients with HBV-DNA 10-2000 IU/mL compared to patients with HBV-DNA >2000 IU/mL (p<0.05). ALT and AST values were significantly higher in patients with HBV-DNA >2000 IU/mL compared to patients with HBV-DNA negative and HBV-DNA 10-2000 IU/mL. No statistically significant correlation was found between HBV-DNA levels and WBC, HGB, MCV, RDW, GGT, ALP, Total protein, albumin, PT, aPTT, INR values. Conclusion: A significant relationship was found between HBeAg and ALT, AST values and HBV-DNA levels in CHB patients. These parameters can be used together to diagnose CHB disease, establish the stage of infection, decide on treatment and determine the course of the disease.

Anahtar Kelimeler

• HBV-DNA
• HBeAg
• ALT
• Chronic hepatitis B

Etik Beyan

This study was approved by the Non-Interventional Scientific Research Ethics Committee of Ordu University Ethics Committee with protocol number 93 and date 12.07.2024.

Kaynakça

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