Protective role of Curcumin in distant tissue damage (heart, kidney and lung) induced bt liver ischemia-reperfusion in rats

Öz

Purpose: There are many different mechanisms in the pathogenesis of distant tissue injury, the generation of reactive oxygen species is the most frequently observed mechanism. Therefore, in this study, we investigated the protective role of curcumin on remote tissue (heart, kidney and lung) damage induced by experimentally induced liver ischemia reperfusion (I/R). Materials and Methods: The study, 24 Wistar-Albino rats were used. Three groups(n=8) consisted on Sham, I/R, Curcumin. The rats were opened through a midline incision and the hepatic portal vein and hepatic artery leading to the liver were closed with a bulldog clamp. The I/R group was subjected to 45minutes of ischemia followed by 45minutes of reperfusion. Curcumin at a dose of 100mg/kg was given intraperitoneally to the treatment group. Rats were sacrificed after the experiment for biochemical examination. Reduced glutathione(GSH) and 8-isoprostagladinF2α(8-isoPGF2α) levels in liver, heart, kidney and lung tissue samples were measured to determine remote tissue damage, oxidative stress damage and protective effects of curcumin. Results: GSH levels in liver, heart, kidney and lung tissues were significantly higher and 8-isoPGF2α levels were significantly lower in curcumin group (p<0.05). Conclusion: Intraperitoneal administration of curcumin after liver I/R induction may protect against I/R injury by regulating the functions of both local and distant tissues.

Anahtar Kelimeler

• ischemia
• reperfusion
• curcumin
• liver

Sıçanlarda karaciğer iskemisi-reperfüzyonunun neden olduğu uzak doku hasarında (kalp, böbrek ve akciğer) Kurkuminin koruyucu rolü

Öz

Amaç: Uzak doku hasarının patogenezinde birçok farklı mekanizma vardır ve reaktif oksijen türlerinin oluşumu en sık gözlenen mekanizmadır. Bu nedenle, bu çalışmada, deneysel olarak indüklenen karaciğer iskemi reperfüzyonu (I/R) tarafından indüklenen uzak doku (kalp, böbrek ve akciğer) hasarı üzerinde kurkuminin koruyucu rolünü araştırdık. Materyal ve Metod: Çalışmada 24 Wistar-Albino sıçan kullanıldı. Üç grup (n=8) Sham, I/R ve Kurkumin'den oluşuyordu. Üç grup (n=8) Sham, I/R ve Curcumin'den oluşmuştur. Sıçanlar orta hat kesisi ile açılmış ve karaciğere giden hepatik portal ven ve hepatik arter bulldog klemp ile kapatılmıştır. I/R grubu 45 dakika iskemiye ve ardından 45 dakika reperfüzyona tabi tutulmuştur. Tedavi grubuna 100 mg/kg dozunda kurkumin intraperitoneal olarak verildi. Deneyden sonra sıçanlar biyokimyasal inceleme için öldürüldü. Karaciğer, kalp, böbrek ve akciğer doku örneklerinde indirgenmiş glutatyon (GSH) ve 8-izoprostagladinF2α (8-isoPGF2α) düzeyleri ölçülerek uzak doku hasarı, oksidatif stres hasarı ve kurkuminin koruyucu etkileri belirlendi. Bulgular: Karaciğer, kalp, böbrek ve akciğer dokularındaki GSH düzeyleri kurkumin grubunda anlamlı olarak daha yüksek, 8-isoPGF2α düzeyleri ise anlamlı olarak daha düşüktü (p<0,05). Sonuç: Karaciğer I/R indüksiyonundan sonra intraperitoneal kurkumin uygulaması, hem lokal hem de uzak dokuların işlevlerini düzenleyerek I/R hasarına karşı koruma sağlayabilir.

Anahtar Kelimeler

• iskemi
• reperfüzyon
• kurkumin
• karaciğer

Kaynakça

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