The Impact of 3G Mobile Phone Base Station Radiation on 14-3-3 Family Proteins in Hepatocellular Carcinoma Cell Line

Yıl 2018, , 87 - 91, 20.03.2018

Öğünç Meral , Mert Pekcan Elçin Özgür , Görkem Kısmalı Deniz Demirkıran Göknur Güler Öztürk Nesrin Seyhan

https://doi.org/10.18663/tjcl.348452

Öz

Aim: The widespread increase of
mobile telecommunication services is mainly responsible for the amount of
radiofrequency radiation (RFR) in public and residential areas. Third
generation (3G) is different from second generation (2G) system technologies
with respect to carrier frequency. 14-3-3
proteins are a family of highly conserved cellular proteins that plays a
crucial role in the regulation of metabolic pathways. Besides 14-3-3 proteins regulate many cellular
processes such as cell growth, differentiation and apoptotic cell death.
The aim was to study the possible effects of third
generation mobile phone base station like Radiofrequency Radiation exposure on
the 14-3-3 protein expression in the hepatocellular carcinoma cells (HepG2).

Materials and Methods: HepG2
cells were exposed RFR  in four different
periods as 1 hour, 2, 3 and 4 hours and then western blot analysis was
performed to analyse protein expression level.

Results: Expressions of 14-3-3
protein were found unaltered in the control, sham and exposure groups.

Conclusion: This
report presents data about the effects of 3G system technologies mobile phone
base station radiation on 14-3-3 protein expression. 14-3-3 isoenzyme analysis
and long term exposure experiments should be done to understand the exact
effects of 3G mobile phone radiation on HepG2 cell line.

Anahtar Kelimeler

14-3-3 protein family, Hepatocellular carcinoma, Radiofrequency

Baz istasyonundan yayılan 3G cep telefonu radyasyonunun hepatoselüler karsinoma hücre hattında 14-3-3 Protein Ailesine etkisi

Öz

Amaç: Mobil telekomünikasyon servislerindeki yaygın artış,
halka açık alanlar ve konutlardaki radyofrekans radyasyon (RFR) miktarının başlıca
sorumlusudur. Üçüncü nesil (3G) sistem teknolojileri ikinci nesil (2G)
teknolojilere göre taşıyıcı frekansları bakımından oldukça farklıdırlar. 14-3-3
protein ailesi metabolik yolların düzenlenmesinde önemli rollere sahip hücresel
proteinleri içerir. Bununla beraber 14-3-3 protein ailesi hücre büyümesi,
farklılaşması ve apoptotik hücre ölümü gibi birçok hücresel süreçte görev
alırlar. Çalışmanın amacı 3G cep telefonu baz istasyonundan yayılan radyofrekans
radyasyona maruz kalan hepatoselüler karsinoma hücrelerinde (HepG2) 14-3-3
protein ekspresyonunun incelenmesidir.

Gereç ve Yöntemler: HepG2 hücreleri 1, 2, 3, ve 4 saat olmak üzere dört farklı periyotta radyofrekans
radyasyona maruz bırakılmış ve protein ekspresyonundaki farklılıkların
incelenmesi için western blot analizi gerçekleştirilmiştir.

Bulgular: Kontrol, sham ve maruziyet gruplarında 14-3-3 protein ekspresyonunda değişime
rastlanmamıştır.

Sonuçlar: Bu çalışmada üçüncü nesil cep telefonu baz istasyonundan yayılan
radyasyonun 14-3-3 protein ekspresyonuna etkisi ile ilgili veriler elde
edilmiştir. 14-3-3 izoenzim analizleri ve uzun süreli maruziyeti içeren
çalışmaların yapılması ile üçüncü nesil cep telefonundan yayılan radyasyonun
HepG2 hücre hattına etkisinin daha iyi anlaşılmasına olanak sağlanacaktır.

Anahtar Kelimeler

14-3-3 protein ailesi, Hepatoselüler karsinom, Radyofrekans

Kaynakça

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