Bactericidal and antibiofilm activities of copper against biofilm producer pathogens colonized on orthopedic implants

Yıl 2018, Cilt: 9 Sayı: 1, 13 - 18, 01.03.2018

Sahra Kırmusaoğlu

https://doi.org/10.18663/tjcl.300359

Öz

Background:
New and alternative antimicrobial and antibiofilm agent discovery has gained attention since antibiotic resistance
was easily developed. It has been accepted that metals have
antimicrobial activity. Abiotic surfaces such as orthopedic implants that are
impregnated with copper can prevent colonization of biofilm producer pathogens,
and can detach biofilms produced on implants. In this study, the effects of
copper against planktonic bacteria and biofilm embedded bacteria adhered on kirschner
wire orthopedic
implant were studied.

Material
and Methods: MICs, MBCs and MBEC of copper against main biofilm producer pathogens
such as methicillin resistance Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA), methicillin
resistance Staphylococcus epidermidis (MRSE), methicillin sensitive Staphylococcus epidermidis (MSSE),
Escherichia coli (E. coli),
Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P.
aeruginosa), Proteus mirabilis (P. mirabilis) colonized on kirschner wire orthopedic implant were determined.

Results:
MICs,
MBCs, and MBECs of copper against pathogens were ranged from 0.063 to 0.75
mg/mL. This study revealed that 0.75
mg/mL of copper inhibit all isolates
analyzed in this study. The most tolerant pathogen was MRSA. The activities of
copper against biofilm embedded
bacteria and planktonic bacteria were found to be the same.

Conclusion:
Indwelling
medical devices such as orthopedic wires, prosthetics can be impregnated by
copper to overcome colonization and production of matured biofilm on indwelling
devices, consequently, implant associated infections.

 

Anahtar Kelimeler

Biofilm, biofilm embedded pathogens, copper, implants, antibiofilm, antimicrobial

Ortopedik implantlar üzerinde kolonize olan biyofilm üreten patojenler üzerinde bakırın bakterisidal ve antibiyofilm aktivitesi

Öz

Amaç: Antibiyotik direnci kolaylıkla geliştiğinden beri, yeni,
alternatif antimikrobiyal ve antibiyofilm ajan keşfi dikkat çekmektedir. Metallerin antimikrobiyal aktivitelere sahip olduğu kabul edilmiştir.
Bakır ile emdirilmiş ortopedik implantlar gibi abiyotik yüzeyler, biyofilm
üreten patojenlerin kolonizasyonunu önleyebilir ve implant üzerinde oluşturulan
biyofilmleri ayırabilir. Bu çalışmada, bakırın planktonik bakteriler ve
kirschner teli ortopedik
implantı üzerine yapışan biyofilme gömülü bakterilere karşı etkisi
çalışıldı.

Gereç ve Yöntemler: Kirschner teli ortopedik implant üzerinde kolonize olan metisilin
dirençli Staphylococcus aureus (MRSA), metisilin duyarlı Staphylococcus aureus (MSSA), metisilin dirençli
Staphylococcus epidermidis (MRSE), metisilin duyarlı Staphylococcus epidermidis (MSSE), Escherichia
coli (E. coli),
Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P.
aeruginosa), Proteus mirabilis (P. mirabilis) gibi ana
biyofilm oluşturan patojenler üzerinde bakırın MIK, MBK ve MBEK değerleri
belirlendi.

Bulgular: Bakırın, patojenlere karşı MIK, MBK ve MBEK
değerleri 0.063 - 0.75 mg/mL arasında değişmektedir. Bu
çalışma, 0.75 mg/mL bakırın çalışmada analiz edilen tüm izolatları inhibe
ettiğini göstermiştir. En dirençli patojen MRSA idi. Bakırın biyofilme gömülü
bakteriler ve planktonik bakteriler üzerindeki aktivitesi aynıydı.

Sonuç: Ortopedik
teller, protezler gibi yabancı
medikal cisimler, yabancı cisimler üzerinde kolonizasyon ve olgun biyofilm
oluşturulmasını önlemek için bakır ile emdirilebilir.

Anahtar Kelimeler

Biyofilm, biyofilme gömülü patojenler, bakır, implantlar, antibiyofilm, antimikrobiyal

Kaynakça

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