Investigation of whole body extract metabolites of Lucilia sericata larvae and potential antibacterial effects

Yıl 2018, Cilt: 9 Sayı: 3, 191 - 198, 30.09.2018

Ali Korhan Sig , Ozgur Koru Engin Araz

https://doi.org/10.18663/tjcl.396308

Öz

Aim:
Complementary medicinal techniques have gainedfocus by modern medicine,
recently. Maggot Debridement Therapy is a widely-used method worldwide. It is
especially recommended for chronic wounds, and has serious advantages such as
low cost, easily-applicability and rare adverse effects, but its effect
mechanisms remains unclear. The aim of this study is to detect components and
to investigate potential antibacterial effects of whole body extract
metabolites of Lucilia sericata
larvae.

Material anf Methods:
Due to potential antibacterial effects, agar well diffusion and flowcytometry
methods were used against Staphylococcus
aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis to do evaluation
on whole body extracts of previously-cloned maggots in specialized climate
room. After this antibacterial effect evaluation, 2-D PAGE analysis was done
for protein investigation.

Results:
Inhibition zones were observed for S.aureus
(16mm), E.coli (22mm) and E.faecalis (14mm), but for P.aeruginosa, the extract could not
provide any inhibiton zone. In flow cytometry, different killing rates were
detected in different extract dilutions, and for the lowest (1/64) dilution,
killing rates were 51.9%, 75%, 80% and 98.7% for P.aeruginosa, E.faecalis, E.coli and S.aureus, respectively. 2-D PAGE showed various proteins with
different molercular mass (<10-260kDa) and pI (3-9).

Conclusion:
Antibacterial effects of maggot whole body extracts on tested strains are
obviously detected. Many protein spots with widely variable molecular mass and
isoelectric points were observed. As a result, this antibacterial effects may
be caused by these proteins, but it is necessary that these proteins must be
further evaluated via mass spectrometry and protein databases.

Anahtar Kelimeler

Lucilia sericata; Chronic Wound Care; Larval Debridement; Biosurgery; Maggot Debridement Therapy

Lucilia sericata larvalarının tüm vücut ekstrakt metabolitlerinin araştırılması ve potensiyel antibakteriyel etkileri

Öz

Amaç:
Yakın dönemde, tamamlayıcı tıp uygulamaları modern tıbbın ilgi alanına
girmiştir. Maggot Debritman Tedavisi dünya çapında yaygın olarak kullanılan bir
yöntemdir. Bu yöntem, özellikle kronik yaraların tedavisinde tavsiye
edilmektedir ve düşük maliyet, kolay uygulanabilirlik ve nadir yan etkiler gibi
avantajları bulunmaktadır, ancak yöntemin etki mekanizması henüz tam olarak
ortaya konulamamıştır. Bu çalışmanın amacı, Lucilia
sericatalarvalarının tüm vücut ekstraktının metabolitlerini ortaya koymak
ve bunların potensiyel antibakteriyel niteliğini araştırmaktır.

Gereç ve Yöntemler:
Antibakteriyel etkinliği araştırmak için, önceden özel iklim odalarında
üretilmiş larvalarının tüm vücut ekstraktları, Staphylococcus aureus, Pseudomonas
aeruginosa, Escherichia coliveEnterococcus faecalisbakterileri için
agar difüzyon ve akan hücreölçer ile test edilmiştir. Antibakteriyel incelemeyi
takiben, iki boyutlu elektroforez ile protein araştırılması yapılmıştır.

Bulgular:
S.aureus (16mm), E.coli (22mm) ve E.faecalis
(14mm) için inhibisyon alanı gözlenmiş ancak P.aeruginosaiçinalan oluşmamıştır. Hücreölçer ile farklı
dilüsyonlarda farklı öldürme oranları gözlenmiş ve en düşük dilüsyonda (1/64), P.aeruginosa, E.faecalis, E.coliveS.aureusiçin sırasıyla%51,9, %75, %80ve
%98,7 oranları alınmıştır. İki boyutlu elektroforezde farklı moleküler ağırlık (<10-260kDa)
ve izoelektrik noktada (3-9) proteinler tespit edilmiştir.

Sonuç:
Maggot ekstraklarının test edilen suşlar üzerine antibakteriyel etkisi net
olarak gözlenmiştir. Farklı moleküler ağırlık ve izoelektrik noktada proteinler
tespit edilmiştir. Antibakteriyel etkinin bu proteinler tarafından sağlanması
muhtemel olsa da, proteinlerin kütle spektrometrisi ve protein veri bankaları
ile ayrıca araştırılması gerekir.

Anahtar Kelimeler

Lucilia sericata; Kronik Yara Bakımı; Larval Debritman; Biyocerrahi; Maggot Debritman Tedavisi

Kaynakça

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