Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme

Yıl 2019, Cilt: 4 Sayı: 1, 15 - 21, 16.05.2019

Aykut Öztekin

Öz

Peroxidases
(PODs) are a group of enzymes that are commonly found in bacteria, fungi,
plants and animals and have important uses in the food, pharmaceutical industry
and clinical diagnostics. Lactoperoxidase (LPO) enzyme, which is included in
peroxidase enzyme class, is found milk, saliva and tears in mammals. With
thiocyanate and hydrogen peroxide, it forms one of the body's defense systems
against infections. Flavonoid derivatives are abundant in plants and constitute
an important part of the diet. These derivatives, which are produced synthetically
or naturally, have many pharmacological activities. In this study, the effect
of some flavonoid derivatives
(5,7-Dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-4H-chromen-4-one (a),
3,5,7-Trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one (b), 7-Hydroxy-4'-nitroisoflavone (c), 6-Fluoroflavone (d),
7-Hydroxy-3-(4-methoxyphenyl)-4H-chromen-4-one (e), 7-Methoxy-2-phenyl-4H-chromen-4-one (f)) on the lactoperoxidase enzyme was investigated. Firstly, by using Sepharose-4B-L-tyrosine-sulfanilamide
affinity chromatography, the LPO enzyme was purified 65 fold (with a yield of 23%)
from bovine milk and kinetic studies were carried out with flavonoid
derivatives using this enzyme. The K_i
values of six molecules were found in ranging from 7.85 µM to 0.023 μM. 6-Fluoroflavone
was the most effective inhibitor with K_i
value of 0.023 μM.

Anahtar Kelimeler

Lactoperoxidase, flavonoid derivatives, inhibition

Kaynakça

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