Araştırma Makalesi
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Yıl 2019, Cilt: 4 Sayı: 1, 15 - 21, 16.05.2019

Öz

Kaynakça

  • Galende, P.P., et al., Purification and structural stability of white Spanish broom (Cytisus multiflorus) peroxidase. 2015. 72: p. 718-723.
  • Thomas, E.J.P.i.c. and biology, Lactoperoxidase: structure and catalytic properties. 1991. 1: p. 123-142.
  • Naidu, A., Lactoperoxidase, in Natural food antimicrobial systems. 2000, CRC Press. p. 116-145.
  • Koes, R., W. Verweij, and F.J.T.i.p.s. Quattrocchio, Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. 2005. 10(5): p. 236-242.
  • Li, R.-S., et al., Design, synthesis and evaluation of flavonoid derivatives as potential multifunctional acetylcholinesterase inhibitors against Alzheimer’s disease. 2013. 23(9): p. 2636-2641.
  • Birt, D.F. and E. Jeffery, Flavonoids. Advances in Nutrition, 2013. 4(5): p. 576-577.
  • Hoensch, H.P. and R.J.W.j.o.g.o. Oertel, Emerging role of bioflavonoids in gastroenterology: Especially their effects on intestinal neoplasia. 2011. 3(5): p. 71.
  • Haenen, G.R. and A. Bast, [50] Nitric oxide radical scavenging of flavonoids, in Methods in enzymology. 1999, Elsevier. p. 490-503.
  • Knekt, P., et al., Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. 1997. 146(3): p. 223-230.
  • Pandey, A.K., et al., Datamining clustering techniques in the prediction of heart disease using attribute selection method. 2013. 14: p. 16-17.
  • Xu, J.-D., L.-W. Zhang, and Y.-F.J.C.C.L. Liu, Synthesis and antioxidant activities of flavonoids derivatives, troxerutin and 3’, 4’, 7-triacetoxyethoxyquercetin. 2013. 24(3): p. 223-226.
  • Aslani, B.A. and S.J.L.s. Ghobadi, Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. 2016. 146: p. 163-173.
  • Shindler, J.S., W.G.J.B. Bardsley, and B.R. Communications, Steady-state kinetics of lactoperoxidase with ABTS as chromogen. 1975. 67(4): p. 1307-1312.
  • Atasever, A., et al., One-step purification of lactoperoxidase from bovine milk by affinity chromatography. Food chemistry, 2013. 136(2): p. 864-870.
  • Markwell, M.A.K., et al., A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical biochemistry, 1978. 87(1): p. 206-210.
  • Lineweaver, H. and D. Burk, The determination of enzyme dissociation constants. Journal of the American chemical society, 1934. 56(3): p. 658-666.
  • Shoaib, M., et al., In vitro enzyme inhibition potentials and antioxidant activity of synthetic flavone derivatives. 2015. 2015.
  • Balasuriya, B.N., H.V.J.F.F.i.H. Rupasinghe, and Disease, Plant flavonoids as angiotensin converting enzyme inhibitors in regulation of hypertension. 2011. 1(5): p. 172-188.
  • Jia, W.Z., et al., Rapid synthesis of flavone‐based monoamine oxidase (MAO) inhibitors targeting two active sites using click chemistry. 2017. 89(1): p. 141-151.
  • Ramirez, G., et al., Chrysoeriol and other polyphenols from Tecoma stans with lipase inhibitory activity. 2016. 185: p. 1-8.
  • Cruz, I., et al., Xanthone and flavone derivatives as dual agents with acetylcholinesterase inhibition and antioxidant activity as potential anti-alzheimer agents. 2017. 2017.
  • Sarikaya, S.B.O., et al., Inhibition profile of a series of phenolic acids on bovine lactoperoxidase enzyme. Journal of enzyme inhibition and medicinal chemistry, 2015. 30(3): p. 479-483.

Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme

Yıl 2019, Cilt: 4 Sayı: 1, 15 - 21, 16.05.2019

Öz

Peroxidases
(PODs) are a group of enzymes that are commonly found in bacteria, fungi,
plants and animals and have important uses in the food, pharmaceutical industry
and clinical diagnostics. Lactoperoxidase (LPO) enzyme, which is included in
peroxidase enzyme class, is found milk, saliva and tears in mammals. With
thiocyanate and hydrogen peroxide, it forms one of the body's defense systems
against infections. Flavonoid derivatives are abundant in plants and constitute
an important part of the diet. These derivatives, which are produced synthetically
or naturally, have many pharmacological activities. In this study, the effect
of some flavonoid derivatives
(5,7-Dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-4H-chromen-4-one (a),
3,5,7-Trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one (b), 7-Hydroxy-4'-nitroisoflavone (c), 6-Fluoroflavone (d),
7-Hydroxy-3-(4-methoxyphenyl)-4H-chromen-4-one (e), 7-Methoxy-2-phenyl-4H-chromen-4-one (f)) on the lactoperoxidase enzyme was investigated. Firstly, by using Sepharose-4B-L-tyrosine-sulfanilamide
affinity chromatography, the LPO enzyme was purified 65 fold (with a yield of 23%)
from bovine milk and kinetic studies were carried out with flavonoid
derivatives using this enzyme
. The Ki
values of six molecules were found in ranging from 7.85 µM to 0.023 μM.
6-Fluoroflavone
was the most effective inhibitor with Ki
value of 0.023 μM.

Kaynakça

  • Galende, P.P., et al., Purification and structural stability of white Spanish broom (Cytisus multiflorus) peroxidase. 2015. 72: p. 718-723.
  • Thomas, E.J.P.i.c. and biology, Lactoperoxidase: structure and catalytic properties. 1991. 1: p. 123-142.
  • Naidu, A., Lactoperoxidase, in Natural food antimicrobial systems. 2000, CRC Press. p. 116-145.
  • Koes, R., W. Verweij, and F.J.T.i.p.s. Quattrocchio, Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. 2005. 10(5): p. 236-242.
  • Li, R.-S., et al., Design, synthesis and evaluation of flavonoid derivatives as potential multifunctional acetylcholinesterase inhibitors against Alzheimer’s disease. 2013. 23(9): p. 2636-2641.
  • Birt, D.F. and E. Jeffery, Flavonoids. Advances in Nutrition, 2013. 4(5): p. 576-577.
  • Hoensch, H.P. and R.J.W.j.o.g.o. Oertel, Emerging role of bioflavonoids in gastroenterology: Especially their effects on intestinal neoplasia. 2011. 3(5): p. 71.
  • Haenen, G.R. and A. Bast, [50] Nitric oxide radical scavenging of flavonoids, in Methods in enzymology. 1999, Elsevier. p. 490-503.
  • Knekt, P., et al., Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. 1997. 146(3): p. 223-230.
  • Pandey, A.K., et al., Datamining clustering techniques in the prediction of heart disease using attribute selection method. 2013. 14: p. 16-17.
  • Xu, J.-D., L.-W. Zhang, and Y.-F.J.C.C.L. Liu, Synthesis and antioxidant activities of flavonoids derivatives, troxerutin and 3’, 4’, 7-triacetoxyethoxyquercetin. 2013. 24(3): p. 223-226.
  • Aslani, B.A. and S.J.L.s. Ghobadi, Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. 2016. 146: p. 163-173.
  • Shindler, J.S., W.G.J.B. Bardsley, and B.R. Communications, Steady-state kinetics of lactoperoxidase with ABTS as chromogen. 1975. 67(4): p. 1307-1312.
  • Atasever, A., et al., One-step purification of lactoperoxidase from bovine milk by affinity chromatography. Food chemistry, 2013. 136(2): p. 864-870.
  • Markwell, M.A.K., et al., A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical biochemistry, 1978. 87(1): p. 206-210.
  • Lineweaver, H. and D. Burk, The determination of enzyme dissociation constants. Journal of the American chemical society, 1934. 56(3): p. 658-666.
  • Shoaib, M., et al., In vitro enzyme inhibition potentials and antioxidant activity of synthetic flavone derivatives. 2015. 2015.
  • Balasuriya, B.N., H.V.J.F.F.i.H. Rupasinghe, and Disease, Plant flavonoids as angiotensin converting enzyme inhibitors in regulation of hypertension. 2011. 1(5): p. 172-188.
  • Jia, W.Z., et al., Rapid synthesis of flavone‐based monoamine oxidase (MAO) inhibitors targeting two active sites using click chemistry. 2017. 89(1): p. 141-151.
  • Ramirez, G., et al., Chrysoeriol and other polyphenols from Tecoma stans with lipase inhibitory activity. 2016. 185: p. 1-8.
  • Cruz, I., et al., Xanthone and flavone derivatives as dual agents with acetylcholinesterase inhibition and antioxidant activity as potential anti-alzheimer agents. 2017. 2017.
  • Sarikaya, S.B.O., et al., Inhibition profile of a series of phenolic acids on bovine lactoperoxidase enzyme. Journal of enzyme inhibition and medicinal chemistry, 2015. 30(3): p. 479-483.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Volume IV, Issue I, 2019
Yazarlar

Aykut Öztekin 0000-0003-1418-179X

Yayımlanma Tarihi 16 Mayıs 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 4 Sayı: 1

Kaynak Göster

APA Öztekin, A. (2019). Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. Turkish Journal of Science, 4(1), 15-21.
AMA Öztekin A. Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. TJOS. Mayıs 2019;4(1):15-21.
Chicago Öztekin, Aykut. “Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme”. Turkish Journal of Science 4, sy. 1 (Mayıs 2019): 15-21.
EndNote Öztekin A (01 Mayıs 2019) Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. Turkish Journal of Science 4 1 15–21.
IEEE A. Öztekin, “Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme”, TJOS, c. 4, sy. 1, ss. 15–21, 2019.
ISNAD Öztekin, Aykut. “Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme”. Turkish Journal of Science 4/1 (Mayıs 2019), 15-21.
JAMA Öztekin A. Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. TJOS. 2019;4:15–21.
MLA Öztekin, Aykut. “Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme”. Turkish Journal of Science, c. 4, sy. 1, 2019, ss. 15-21.
Vancouver Öztekin A. Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. TJOS. 2019;4(1):15-21.