The frequency of respiratory tract viral agents in children with lower respiratory tract infections

Yıl 2013, Cilt: 48 Sayı: 3, 215 - 220, 01.10.2013

Sinem Akçalı Nisel Yılmaz Özlem Güler Tamer Şanlidağ1 Murat Anıl

Öz

Aim: Lower respiratory tract infections LRTI have high morbidity rates in children In this study it was aimed to investigate the prevalence of respiratory viruses in children with LRTI symptoms Material and Method: A total of 160 children who were diagnosed with LRTI between October 2009 and March 2010 were included in the study The presence of respiratory syncytial virus RSV A B influenza virus A B parainfluenza virus PIV 1 2 3 4 human metapneumovirus rhinovirus and coronavirus OC43 229E in throat swab samples were investigated by real time PCR The RealAccurateTM Respiratory RT PCR Kit PathoFinder B V Netherlands Results: In 67 samples 41 8 at least one virus which could cause acute respiratory tract infection was found Overall RSV was the most frequently identified virus 52 2 followed by rhinovirus 26 8 coronavirus 5 metapneumovirus 2 9 and PIV 1 1 4 As the other viral agents coronavirus was detected in 4 samples 5 hMPV was detected in 2 samples 2 9 and PIV was detected in 1 sample 1 4 When the frequency of coinfections was evaluated RSV Rhinovirus association was found in 4 samples RSV Coronavirus association was found in 1 sample Rhinovirus Coronavirus association was found in 1 sample and RSV Rhinovirus Coronavirus association was found in 1 sample Conclusions: In 41 8 of the study group a viral factor responsible for the clinical signs was detected For that reason rapid and sensitive diagnosis of viruses which lead to respiratory infections will guide the clinician for avoidance of redundant antibiotic therapy and preventing viral hospital infections Turk Arch Ped 2013; 48: 215 220

Anahtar Kelimeler

Children, respiratory tract infections, real-time PCR, viruses

Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı

Öz

Amaç: Alt solunum yolu enfeksiyonları çocuklarda sık görülür ve özellikle erken çocukluk çağında ölüm oranı yüksektir Bu araştırmada bölgemizde alt solunum yolu enfeksiyonları belirtileri bulunan çocuklarda solunum yolu viral etkenlerinin sıklığının belirlenmesi amaçlanmıştır.

Gereç ve Yöntem: Çalışma grubu Ekim 2009 Mart 2010 tarihleri arasında alt solunum yolu enfeksiyonları belirtileri ile hastaneye başvuran 160 çocuktan ort 14 6 ay oluşturulmuştur Bu çocuklardan alınan boğaz sürüntü örneklerinde respiratuar sinsityal virüs A B influenza virüs A B parainfluenza virüs 1 2 3 4 insan metapnömovirüs rinovirüs ve koronavirüs OC43 229E varlığı real time PCR yöntemiyle [The RealAccurateTM Respiratory RT PCR Kit PathoFinder BV Hollanda ] araştırılmıştır.

Bulgular: Örneklerin 67 rsquo;sinde 41 8 alt solunum yolu enfeksiyonları belirtilerine neden olabilecek bir viral etken saptanmıştır Respiratuar sinsityal virüs 61 2 rsquo;lik oranla 41 67 en sık saptanan virüs olmuştur Bunu 35 8 24 67 ile rinovirüsler izlemektedir Dört örnekte 5 koronavirüs iki örnekte 2 9 ldquo;insan metapnömovirüs rdquo; bir örnekte de 1 4 parainfluenza virüs diğer viral etkenler olarak belirlenmiştir Birlikte enfeksiyon sıklıkları değerlendirildiğinde dört örnekte respiratuar sinsityal virüs rinovirüs birlikteliği bir örnekte respiratuar sinsityal virüs koronavirüs birlikteliği bir örnekte rinovirüs koronavirüs birlikteliği bir örnekte de respiratuar sinsityal virüs rinovirüs koronavirüs birlikteliği saptanmıştır. 

Çıkarımlar: Çalışma grubunun 41 8 rsquo;inde klinik belirtilerden sorumlu olabilecek bir viral etken saptanmıştır Bu nedenle viral solunum yolu enfeksiyonlarına neden olan virüslerin hızlı ve duyarlı tanısı gereksiz antibiyotik kullanımının engellenebilmesi ve bu virüslerin neden olabilecekleri hastane enfeksiyonlarının önlenebilmesi açısından klinisyene yol gösterici olacaktır.

Anahtar Kelimeler

Çocuk, solunum yolu enfeksiyonları, real-time PCR, virüsler

Kaynakça

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