Myotis myotis’ DE (CHİROPTERA: VESPERTİLİONİDAE) İMPLANTASYONUN HİSTOLOJİK OLARAK İNCELENMESİ

Öz

Bu çalışmada, Myotis myotis türü yarasalarda implantasyon histolojik olarak ışık mikroskobunda (Olympus BX51) incelenmiştir. Dişi yarasa örnekleri Kırklareli’ de bulunan Koyunbaba Mağarası’ndan yakalanmıştır. Myotis myotis türü yarasa örneklerinin uteruslarından alınan histolojik kesitler, Hematoksilen-Eosin (Mayer’s), Gomori trichrome ile boyanmış ve incelenmiştir. Myotis myotis’de uterus bicornis’ dir ve implantasyonun uterusun sağ boynuzunda gerçekleştiği gözlenmiştir. Embriyonik disk amnion boşluğunun taban kısmında yer almıştır. Trofoblast, sitotrofoblast ve sinsisyotrofoblast tabakalarına farklılaşmıştır.

Anahtar Kelimeler

• Chiroptera, placenta, Myotis myotis, embriyo

HISTOLOGICAL INVESTIGATION of IMPLANTATION in Myotis myotis (CHİROPTERA: VESPERTİLİONİDAE)

Öz

In this study, implantation in Myotis myotis was examined histologically by light microscopy (Olympus BX51). İndividuals of female bats were captured from the Koyunbaba Cave in Kırklareli. Histological sections of uterus of Myotis myotis were stained with Hematoxylin-Eosin (Mayer’s), Gomori trichrome and examined. We observed that Myotis myotis has a bicornuate uterus and implantation occurs in the right horn. Embriyonic disc is located at the basal side of the amniotic cavity. Trophoblast differentiated to cytotrophoblast and syncytiotrophoblast.

Anahtar Kelimeler

• Chiroptera, placenta, Myotis myotis, embryo

Kaynakça

1. 1. ALTRINGHAM JD. Bats: Biology and behavior. Oxford University Press, p. 264, Oxford, 1996.
2. 2. AMOROSO EC. Placentation. Pp: 127–311, In: Parkes AS, editor. Marshall’s physiology of reproduction. London: Longmans, Green, 1952.
3. 3. BADWAIK NK, RASWEİLER JJIV. Pregnancy. Pp: 221–293. In: Crichton EG, Krutzch PH, editors. Reproductive biology of bats. Academic Press. SanDiego, 2000.
4. 4. BANCROFT JD, COOK HC. Manual of Histological Techniques and Their Diagnostic Application. Churchill Livingstone, p. 457, London, 1994.
5. 5. CARTER AM, GOODMAN SM, ENDERS AC. Female reproductive tract and placentation in suckerfooted bats (Chiroptera: Myzopodidae) endemic to Madagascar. Placenta. 29(6); 484-91, 2008.
6. 6. DEANESLY R, WARWICK T. Observations on pregnancy in the common bat (Pipistrellus pipistrellus). Proceedings of the Zoological Society of London. Series A 109; 57–60, 1939.
7. 7. ELANGOVAN V, RAGHURAM H, YUVANA SATYA PRIYA E, MARIMUTHU G. Wing morphology and flight development in the short-nosed fruit bat Cynopterus sphinx. Zoology. 110; 189–196, 2007.
8. 8. ENDERS AC. A comparative study of the fine structure of the trophoblast in several hemochorial placentas. American Journal of Anatomy. 116; 29–68, 1965.

9. 9. GOPALAKRISHNA A, KARIM KB. Female genital anatomy and the morphogenesis of foetal membranes of Chiroptera and their bearing on the phylogenetic relationships of the group. Pp: 379-428. In: National Academy of Sciences, India: Golden Jubilee Commemoration Volume. U.S. Srivastava, ed. Naya Prokash, Allahabad, India, 1980.
10. 10. HEIDEMAN PD, CUMMINGS JA, HEANEY LR. Reproductive timing and early embryonic development in the Old World fruit bat, Otopteropus cartilagonodus (Megachiroptera). Journal of Mammalogy. 74; 621– 30, 1993.
11. 11. HEIDEMAN PD, POWELL KS. Age-specific reproductive strategies and delayed embryonic development in an old world fruit bat, Ptenochirus jargori. Journal of Mammalogy. 79; 295–311, 1998.
12. 12. KARIM KB. Blastocyst–uterus relationship in the Indian rhinopomatid, Rhinopoma hardwickei hardwickei. Pp: 427–34. In Progress in Developmental Biology, Part A, ed.H. C. Slavkin, New York, Alan Liss., 1986.
13. 13. KARIM KB. Embryology of some Indian Chiroptera. DSc dissertation, Nagpur University, India, 1976.
14. 14. KOOPMAN KF. Bats. In: D.E. Wilson and D.M. Reeder, Editors, Mammalian Species of the World. A Taxonomic and Geographic Reference, Smithsonian Institution Press, Washington, 1993.
15. 15. KRUTZSCH PH. Reproduction of the canyon bat, Pipistrellus hesperus, in southwestern United States. The American Journal of Anatomy. 143; 163–200, 1975.
16. 16. KUNZ TH. Reproduction, growth, and mortality of the vespertilionid bat, Eptesicus fuscus, in Kansas. Journal of Mammalogy. 55; 1–13, 1974.
17. 17. KURTA A, KUNZ TH. Size of bats at birth and maternal investment during pregnancy. Symposia of the Zoological Society of London. 57; 79–106, 1987.
18. 18. LUCKETT WP. The use of fetal membrane data in assessing chiropteran phylogeny. Pp: 245–265. In: Wilson DE, Gardner AL, editors. Proceedings Fifth International Bat Research Conference. Lubbock: Texas Tech Press, 1980.
19. 19. MOSSMAN HW. Comparative morphogenesis of the fetal membranes and accessory uterine structures. Contributions to Embryology, Carnegie Institute. 26; 129–246, 1937.
20. 20. MOSSMAN HW. Vertebrate Fetal Membranes: Comparative Ontogeny and Morphology; Evolution; Phylogenetic Significance; Basic Functions; Research Opportunities. New Brunswick: Rutgers University Press, 1987.
21. 21. NEUWEILER G. The biology of bats. Oxford University Press, p. 310, New York, 2000.
22. 22. RACEY PA. The prolonged storage and survival of spermatozoa in Chiroptera. Journal of Reproduction and Fertility. 56; 391–402, 1979.
23. 23. RASWEILER JJIV. Early embryonic development and implantation in bats. Journal of Reproduction and Fertility. 56; 403–16, 1979.
24. 24. RASWEILER JJIV. Pregnancy in Chiroptera. Journal of Experimental Zoology. 266; 495–513, 1993.
25. 25. REEP RL, BHATNAGAR KP. Brain ontogeny and ecomorphology in bats. Pp: 93–136. In: Adams RA, Pedersen SC, editors. Ontogeny, functional ecology, and evolution of bats. Cambridge: Cambridge University Press, 2000.
26. 26. SHERMAN HB. Birth of the young of Myotis austroriparius. Journal of Mammalogy. 11; 495–503, 1930.
27. 27. STARCK D. Ontogenie und Entwicklungsphysiologie der Sa¨ugetiere. Pp: 1–276. In: Ku¨kenthal W, editor. Handbuch der Zoologie, Vol. 8, part 22, 9(7). Berlin: Walter Gruyter, 1959.
28. 28. WIMSATT WA. Notes on breeding behaviour, pregnancy and parturition in some vespertilionid bats of the eastern United States. Journal of Mammalogy. 26; 23–33, 1945.
29. 29. WIMSATT WA. Reproductive asymmetry and unilateral pregnancy in Chiroptera. Journal of Reproduction and Fertility. 56; 345–357, 1979.
30. 30. WIMSATT WA. Some comparative aspects of implantation. Biology of Reproduction. 12; 1-40, 1975