Pepsinogen identification in the middle ear fluid of children with otitis media with effusion

Yıl 2016, Cilt: 26 Sayı: 2, 73 - 78, 10.05.2016

Aytuğ Buyruk Üstün Osma Mustafa Deniz Yılmaz Hülya Eyigör

Öz

Objectives: This study aims to investigate the presence and concentration of pepsin/pepsinogen in middle ear fluid and to discuss the potential mechanisms involved in the pathogenesis of this condition. Patients and Methods: A total of 33 children 21 boys, 12 girls; mean age 5.7±2.4 years; range 3 to 13 years diagnosed with otitis media with effusion and scheduled for operation were enrolled into the study. Fluids aspirated from the middle ear were assessed for the presence of pepsinogen and albumin and blood samples were drawn simultaneously for comparison. Results: Mean pepsinogen concentration was statistically significantly higher in middle ear fluids compared with serum samples 262.4 ng/mL [range: 211.7 ng/mL - 301.1 ng/mL] versus 102.6 ng/mL [range: 80.7 ng/mL - 134.5 ng/mL], respectively p<0.001 . On the other hand, mean albumin concentration was significantly lower 1.1 g/dL [range: 0.01 g/dL - 9.5 g/dL] versus 5.8 g/dL [range: 0.9 - 9.5 g/dL], respectively p<0.001 . The highest pepsinogen concentration was detected in patients with purulent effusion 275.3 ng/mL . Conclusion: Our findings support the theory of gastro-esophageal reflux related pepsinogen transition to the middle ear and indicate that pepsinogen may a reliable biochemical marker for the assessment of gastro-esophageal reflux.

Anahtar Kelimeler

Gastro-esophageal reflux, otitis media with effusion, pepsin, pepsinogen

Efüzyonlu otitis medialı çocukların orta kulak sıvılarında pepsinojenin saptanması

Öz

Amaç: Bu çalışmada orta kulak sıvısında pepsin/pepsinojen varlığı ve konsantrasyonu araştırıldı ve bu durumun patogenezindeki olası mekanizmalar tartışıldı.Hastalar ve Yöntemler: Efüzyonlu otitis media saptanan ve ameliyat planlanan toplam 33 çocuk 21 erkek, 12 kız; ort. yaş 5.7±2.4 yıl; dağılım 3-13 yıl çalışmaya alındı. Pepsinojen ve albumin varlığına ilişkin orta kulaktan aspire edilen sıvılar değerlendirildi ve karşılaştırma için eş zamanlı olarak kan örnekleri alındı.Bulgular: Orta kulak sıvılarında ortalama pepsinojen konsantrasyonu serum örnekleriyle karşılaştırıldığında istatistiksel olarak anlamlı şekilde yüksekti sırasıyla 262.4 ng/mL [dağılım: 211.7 ng/mL - 301.1 ng/mL]’ye kıyasla 102.6 ng/mL [dağılım: 80.7 ng/mL - 134.5 ng/mL] p

Anahtar Kelimeler

Gastro-özofageal reflü, efüzyonlu otitis media, pepsin, pepsinojen

Kaynakça

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