Sağlıklı Genç Yetişkinlerde Akut Tükenme Egzersizinin Dolaşımdaki İrisin Konsantrasyonları Üzerine Etkileri

Öz

Yakın zamanda iskelet kas dokusundan salgılandığı tespit edilen irisin, enerji tüketimini artırarak, metabolizmayı etkileyen miyositler tarafından salgılanan yeni bir hormondur. Bu araştırmanın amacı genç yetişkinlerde akut egzersizinin irisin düzeylerine etkisini belirlemektir. Araştırmaya 20-25 yaş aralığında düzenli olarak egzersiz yapmayan sağlıklı 13 gönüllü erkek katılmıştır. Akut egzersiz öncesi katılımcıların boy uzunluğu ve vücut ağırlığı ölçülerek vücut kütle indeksi, skinfold kaliper kullanılarak vücut yağ yüzdesi hesaplanmıştır. Gönüllülerin akut tükenme egzersizi öncesi ve sonrasında kan örnekleri alınarak plazmaları kazanılmış ve irisin hormon analizleri Enzyme-Linked Immuno Sorbent Assay (ELİSA) yöntemiyle tespit edilmiştir. Verilerin analizinde SPSS 16.0 istatistik paket program kullanılmıştır. Verilerin ortalama ve standart sapmaları hesaplanmış ve ikili küme karşılaştırmaları için bağımsız gruplarda paried simple t-testi kullanılmıştır. Araştırmada anlamlılık düzeyi 0.05 olarak kabul edilmiştir. Çalışma sonucunda dinlenik düzeyi 29,34±4,18 ng/ml olan irisin düzeylerinin akut tükenme egzersizi sonrası 28,45±3,73 ng/ml azaldığı fakat bu durumun istatistiksel olarak önemli olmadığı belirlenmiştir (p>0,05). Sonuç olarak genç yetişkinlerde akut tükenme egzersizinin irisin hormon düzeylerini etkilemediği sonucuna ulaşılmıştır.

Anahtar Kelimeler

• Akut tükenme egzersizi,İrisin,Myokin

Effects of Acute Exhaustion Exercise on Circulating Irisin Concentrations in Healthy Young Adults

Öz

The irisin, which has recently been detected to be secreted from skeletal muscle tissue, is a new hormone secreted by myocytes that affect metabolism by increasing energy consumption. The aim of this study is to determine the effect of acute exhaustion exercise on irisin levels in young adults. Thirteen healthy volunteers who did not exercise regularly between the ages of 20-25 participated in the study. Body mass index was calculated by measuring the height and body weight of the participants and the body fat percentage using the skinfold caliper. Blood samples were obtained before and after acute exhaustion exercise from volunteers and their plasma was obtained and irisin hormone analyzes were determined by Enzyme-Linked Immuno Sorbent Assay (ELISA) method. SPSS 16.0 statistical software package was used for analyzing the data. Average and standard deviations of the data were calculated and paried sample t-test was used in independent groups. In the research, the level of significance was accepted as 0.05. As a result of the study, it was determined that irisin levels, whose rest level was 29.34 ± 4.18 ng / ml, decreased to 28.45 ± 3.73 ng / ml after acute exhaustion exercise, but this was not statistically significant (p> 0.05). As a result, it was concluded that acute exhaustion exercise does not affect the hormone levels of irisin in young adults. 

Anahtar Kelimeler

• Acute exhaustion exercise,irisin,myokin

Kaynakça

1. American College of Sports Medicine. (2000). ACSM’s guidelines for exercise testing and prescription. Sixth Edition. USA. Lippincott. Williams&Wilkins.
2. Akcan, F. (2018). Farklı şiddetlerde uygulanan aerobik ve anaerobik egzersizlerin sporcu ve sedanterlerde serum irisin seviyesine akut etkisinin incelenmesi. Doktora Tezi, Gaziantep Üniversitesi Sağlık Bilimleri Enstitüsü, Gaziantep.
3. Anastasilakis, A. D., Polyzos, S. A., Saridakis, Z. G., Kynigopoulos, G., Skouvaklidou, E. C., Molyvas, D., vd. (2014). Circulating irisin in healthy, young individuals: day-night rhythm, effects of food intake and exercise, and associations with gender, physical activity, diet, and body composition. The Journal of Clinical Endocrinology & Metabolism, 99(9), 3247-3255.
4. Arıkan, Ş. (2018). The effect of acute exercise and gender on the levels of irisin in elite athletes. Physical Education of Students, 22(6), 304-307. Arıkan, Ş., Revan, S., Balcı, Ş. S., Şahin, M. ve Serpek, B. (2018). Effect of training on plasma irisin, leptin and insulin levels. International Journal of Applied Exercise Physiology, 2, 1-8.
5. Benedini, S., Dozio, E., Invernizzi, P. L., Vianello, E., Banfi, G., Terruzzi, I., vd. (2017). Irisin: A potential link between physical exercise and metabolism-an observational study in differently trained subjects, from elite athletesto non-athlete people. Hindawi Journal of Diabetes Research, 1-7.
6. Boström, P., Wu, J., Jedrychowski, M. P., Korde, A., Ye, L., Lo, J. C., vd. (2012). A PGC1-[agr]-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature, 481, 463-468.
7. Choi, Y. K., Kim, M. K., Bae, K. H., vd. (2013). Serum irisin levels in new- onset type 2 diabetes. Diabetes Research and Clinical Practice, 100(1), 96‐101.
8. Daskalopoulou, S. S., Cooke, A. B., Gomez, Y. H., Mutter, A. F., Filippaios, A., Mesfum, E. T., vd. (2014). Plasma irisin levels progressively increase in response to increasing exercise work loads in young, healthy, active subjects. European Journal of Endocrinology, 171(3), 343-352.

9. Durnin, J. V. ve Womersley, J. (1974). Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 years. British Journal of Nutrition, 32(1),77- 97.
10. Egan, B., Carson, B. P., Garcia-Roves, P. M., Chibalin, A. V., Sarsfield, F. M., Barron, N., vd. (2010). Exercise intensity-dependent regulation of peroxisome proliferator-activated receptor coactivator-1 mRNA abundance is associated with differential activation of upstream signaling kinases in human skeletal muscle. The Journal of Physiology, 588(10),1779-1790.
11. Fox, J., Rioux, B. V., Goulet, E. D. B., Johanssen, N. M., Swift, D. L., Bouchard, D. R., vd. (2017). Effect of an acute exercise bout on immediate post- exercise irisin concentration in adults: A meta- analysis. The Scandinavian Journal of Medicine & Science in Sports, 26, 16-28.
12. Huh, J. Y., Mougios, V., Skraparlis, A., Kabasakalis, A. ve Mantzoros, C. S. (2014a). Irisin in response to acute and chronic whole-body vibration exercise in humans. Metabolism, 63(7), 918-21.
13. Huh, J. Y., Mougios, V., Kabasakalis, A., Fatouros, I., Siopi, A., vd. (2014b). Exercise-induced irisin secretion is independent of age or fitness level and increased irisin may directly modulate muscle metabolism through AMPK activation. The Journal of Clinical Endocrinology & Metabolism, 99(11), E2154-E2161.
14. Huh, J. Y., Panagiotou, G., Mougios, V., Brinkoetter, M., Vamvini, M. T., Schneider, B. E. ve Mantzoros, C. S. (2012). FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism, 61, 1725-1738.
15. Huh, J. Y., Siopi, A., Mougios, V., Park, K. H. ve Mantzoros. C. S. (2015). Irisin in response to exercise in humans with and without metabolic syndrome. The Journal of Clinical Endocrinology & Metabolism, 100(3), E453-E457.
16. Kabasakalis, A., Nikolaidis, S., Tsalis, G., Christoulas, K. ve Mougios, V. (2019). Effects of sprint interval exercise dose and sex on circulating irisin and redox status markers in adolescent swimmers. Journal of Sports Sciences, 37(7), 827-832.
17. Khalafi, M., Shabkhız, F., Alamdarı, K. A. ve Bakhtıyarı A. (2016). Irisin responseto two types of exercise training in type 2 diabetic male rats. Arak Medical University Journal, 19, 37-45. Kraemer, R. R., Shockett, P., Webb, N. D., Shan, U. ve Castracana, V. D. (2014). A transient elevated irisin blood concentration in response to prolonged, moderate aerobic exercise in young men and women. Hormone and metabolic research, 46(2), 150-154.
18. Leger, L. A. ve Lambert, J. (1982). A maximal multistage 20-m shuttle run test to predict\ dot VO2 max. European Journal Of Applied Physiology and Occupational Physiology, 49, 1-12.
19. Moreno-Navarrete, J. M., Ortega, F., Serrano, M., vd. (2013). Irisin is expressed and produced by human muscle and adipose tissue in association with obesity and insulin resistance. The Journal of Clinical Endocrinology & Metabolism, 98(4), E769‐E778.
20. Norheim, F., Langleite, T. M., Hjorth, M., Holen, T., Kielland, A., Stadheim, H. K., vd. (2014). The effects of acute and chronic exercise on PGC-1α, irisin and browning of subcutaneous adipose tissue in humans. The FEBS Journal, 281(3), 739-749.
21. Nygaard, H., Slettaløkken, G., Vegge, G., Hollan, I., Whist, J. E., Strand, T., vd. (2015). Irisin in blood increases transiently after single sessions of intense endurance exercise and heavy strength training. PLoS One, 10(3),1-12.
22. Özçelik, O., Algül, S., Deniz, M., Baydaş, F. ve Tan, F. (2017). Sabah ve gece yapılan aerobik egzersizin antrenmanlı deneklerde serum irisin düzeyine etkilerinin belirlenmesi. Genel Tıp Dergisi, 27(1), 1-5.
23. Park, K. H., Zaichenko, L., Brinkoetter, M., vd. (2013). Circulating irisin in relation to insulin resistance and the metabolic syndrome. The Journal of Clinical Endocrinology & Metabolism, 98(12), 4899‐4907.
24. Pedersen, B. K. ve Febbraio, M. A. (2012). Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nature Reviews Endocrinology, 8, 457‐465.
25. Pedersen, B. K. ve Saltin, B. (2015). Exercise as medicine-evidence for prescribing exercise as therapy in 26 different chronic diseases. The Scandinavian Journal of Medicine & Science in Sports, 25(Suppl 3), 1‐72.
26. Pekkala, S., Wiklund, P. K., Hulmi, J. J., Ahtiainen, J. P., Horttanainen, M., Pöllänen, E., vd. (2013). Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health? The Journal of Physiology, 591(21), 5393-5400.
27. Polyzos, S. A., Anastasilakis, A. D., Efstathiadou, Z. A., Makras, P., Perakakis, N., Kountouras, J., vd. (2018). Irisin in metabolic diseases. Endocrine, 2, 260-274.
28. Roca-Rivada, A., Castelao, C., Senin, L. L., vd. (2013). FNDC5/irisin is not only a myokine but also an adipokine. PLoS One, 8(4), e60563. Rodriguez, A., Becerril, S., Mendez-Gimenez, L., vd. (2015). Leptin administration activates irisin- induced myogenesis via nitric oxide- dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice. International Journal of Obesity, 39, 397‐407.
29. Scalzo, R. L., Peltonen, G. L., Giordano, G. R., vd. (2014). Regulators of human white adipose browning: evidence for sympathetic control and sexual dimorphic responses to sprint interval training. PLoS One, 9, 90696.
30. Staiger, H., Bohm, A., Scheler, M., Berti, L., Machann, J., Schick, F., vd. (2013). Common genetic variation in the human FNDC5 locus, encoding the novel muscle-derived ‘browning’ factor irisin, determines insulin sensitivity. PLoS ONE, 8, e61903.
31. Stengel, A., Hofmann, T., Goebel-Stengel, M., Elbelt, U., Kobelt, P. ve Klapp, B.F. (2013) Circulating levels of irisin in patients with anorexia nervosa and different stages of obesity – correlation with body mass index. Peptides, 39, 125-130.
32. Stepto, N. K., Benziane, B., Wadley, G. D., Chibalin, A. V., Canny, B. J., Eynon, N., vd. (2012). Short-term intensified cycle training alters acute and chronic responses of PGC1α and cytochrome C oxidase IV to exercise in human skeletal muscle. PLoS One, 7(12), e53080.
33. Tavassoli, H., Heidarianpour, A. ve Hedayati, M. (2019). The effects of resistance exercise training followed by de-training on irisin and some metabolic parameters in type 2 diabetic rat model. Archives of Physiology and Biochemistry, 7, 1-8.
34. Tsuchiya, Y., Ando, D., Goto, K., Kiuchi, M., Yamakita, M. ve Koyama, K. (2014). High-intensity exercise causes greater irisin response compared with low-intensity exercise under similar energy consumption. The Tohoku Journal of Experimental Medicine, 233, 135-140.
35. Tsuchiya, Y., Ando, D., Takamatsu, K. ve Goto, K. (2015). Resistance exercise induces a greater irisin response than endurance exercise. Metabolism, 64, 1042-1050.
36. Qiu, S., Cai, X., Sun, Z., Schumann, U., Zugel, M. ve Steinacker, J. M. (2015). Chronic exercise training and circulating irisin in adults: A meta- analysis. Sports Medicine, 45, 1577‐1588.