Yeni Tanı Almış Tip 2 Diabetes Mellitus Hastalarında Plazma E Vitamini ve Plazma Nitrit/Nitrat Anyon Düzeylerinin Değerlendirilmesi

Öz

Amaç: Oksidatif stres, tip 2 Diabetes mellitus (T2DM) başlangıcını indükler. Hem plazma E vitamini (EV) hem de nitrit/nitrat anyon (NOx) seviyeleri, farklı mekanizmalar yoluyla diyabetin neden olduğu endotelyal hasarı belirlemek için çok önemli faktörler olabilir. Bu nedenle bu çalışmada yeni tanı almış T2DM hastalarının plazma EV ve NOx konsantrasyonlarını değerlendirmeyi ve hastaların lipid profilleri ile herhangi bir korelasyon olup olmadığını anlamayı amaçladık. Gereç ve Yöntemler: Hastalar (n=15) ve kontroller (n=15) üniversitemiz diyabet polikliniğine sağlık muayenesi için ilk geldiklerinde kan örnekleri alındı ve plazma EV ve NOx düzeylerinin saptanması için kullanıldı. Hasta dosyaları hem demografik verileri hem de biyokimyasal parametreleri kaydetmek için değerlendirildi. Veriler Kolmogorov-Smirnov testine göre normal dağıldı. Bu nedenle hasta ve kontrol verilerinin karşılaştırılmasında Student t testi ve korelasyon analizi için Pearson testi uygulandı. Bulgular: Yeni tanı konmuş T2DM hastalarında total kolesterol (TK) ve trigliserit düzeyleri anlamlı olarak yükselirken (sırasıyla p=0,036 ve p<0,001), HDL-kolesterol düzeyleri ise anlamlı düzeyde azaldı (p<0,001). T2DM hastalarında plazma NOx seviyelerindeki artış TK seviyeleri ile pozitif korelasyon gösterdi (r=0,392, p=0,032). Diyabet grubunda plazma EV seviyeleri azaldı. Plazma EV ve TK (r=-0,415, p=0,022) ve ayrıca trigliserit (r=-0,380, p=0,039) düzeyleri arasında anlamlı bir negatif korelasyon bulundu. Sonuç: Bu çalışma, EV'nin hücre zarı üzerindeki koruyucu etkisinin T2DM'de azaldığını göstermektedir. Plazma NOx seviyelerinin yükselmesi, hiperglisemide artan nitrik oksit sentaz seviyelerine bağlıdır. Plazma EV ve NOx seviyeleri arasında bir ilişki bulunmamakla birlikte, özellikle yüksek TK seviyeleri olan hastalarda T2DM'nin neden olduğu vasküler hasarı anlamada spesifik biyobelirteçler olarak kullanılabilirler.

Anahtar Kelimeler

• E vitamini
• nitritler
• nitratlar
• kolesterol
• diabetes mellitus;tip 2

Proje Numarası

BAP 2015-92436924-01

The Evaluation of Plasma Vitamin E and Plasma Nitrite/Nitrate Anion Levels in Newly Diagnosed Type 2 Diabetes Mellitus Patients

Öz

Aim: Oxidative stress induces the onset of type 2 Diabetes mellitus (T2DM). Both plasma vitamin E (VE) and nitrite/nitrate anion (NOx) levels might be crucial factors to determine diabetes induced endothelial damage through different mechanisms. Therefore, in this study, we aimed to evaluate plasma VE and NOx concentrations of newly diagnosed T2DM patients and to understand whether any correlation exists with the patients’ lipid profiles. Material and Methods: Blood samples were taken when the patients (n=15) and the controls (n=15) first came to our university’s diabetes policlinic for investigation and were used to detect plasma VE and NOx levels. Patients’ files were evaluated to record both the demographic data and biochemical parameters. The data was distributed normally according to Kolmogorov-Smirnov test. Therefore, Student t test was chosen to compare patient and control data and Pearson test was applied for correlation analysis. Results: In newly diagnosed T2DM patients, total cholesterol (TC) and triglyceride levels were found to be significantly elevated (p=0.036, and p<0.001, respectively), whereas, HDL-cholesterol levels were significantly reduced (p<0.001). Plasma NOx levels increased in T2DM patients and this was in positive correlation with TC levels (r=0.392, p=0.032). Plasma VE levels reduced in the diabetic group. A significant negative correlation was found between plasma VE and TC (r=-0.415, p=0.022) as well as triglyceride (r=- 0.380, p=0.039) levels. Conclusion: This study demonstrates that the protective effect of VE on cell membrane decreases in T2DM. The elevation of plasma NOx levels is due to increased nitric oxide synthase levels in hyperglycemia. Although no correlation was found between plasma VE and NOx levels, they can be used as specific biomarkers in understanding T2DM induced vascular damage especially in patients with high TC levels.

Anahtar Kelimeler

• vitamin E
• nitrites
• nitrates
• cholesterol
• diabetes mellitus;type 2

Destekleyen Kurum

Zonguldak Bulent Ecevit University Scientific Research Project (BAP) Unit

Proje Numarası

BAP 2015-92436924-01

Teşekkür

We would like to thank Cinar Severcan (PhD) for his help in statistical analysis.

Kaynakça

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