Gestasyonel Diyabet ile IL8/CXCL8 rs4073 Gen Polimorfizmi Arasındaki İlişkinin Araştırılması

Yıl 2023, , 66 - 71, 28.04.2023

Güneş Çakmak Genç , Sevim Karakaş Çelik , Mürsel Hazaloğlu , Müge Harma , Ahmet Dursun

https://doi.org/10.25048/tudod.1198053

Öz

Amaç: Gestasyonel Diyabet (GDM) ilk defa gebelikte tanı konulan glukoz intoleransıdır. Hem anne hem de bebekte komplikasyon
riskini artıran GDM için ileri gebelik yaşı, çok sayıda parite, önceki gebelikte GDM öyküsü, genetik faktörler gibi birçok risk faktörü
tanımlanmıştır. Ayrıca bazı inflamatuar mediatörler, kemokinler ve onları kodlayan genlerin polimorfizmlerinin GDM ile ilişkisi
gösterilmiştir. Biz bu çalışmada GDM tanısı konulan gebelerle sağlıklı gebelerdeki genotip ve allel frekans dağılımlarını karşılaştırarak,
GDM yatkınlığı ile IL8/CXCL8 rs4073 (251A/T) gen polimorfizminin ilişkisini belirlemeyi amaçladık.
Gereç ve Yöntemler: Çalışmamıza GDM tanılı 100 gebe ve kontrol grubu olarak 100 sağlıklı gebe dahil edilmiştir. Tüm katılımcılar
Zonguldak Bülent Ecevit Üniversitesi Tıp Fakültesi Hastanesi Kadın Hastalıkları ve Doğum Anabilim Dalı’nda takip edilmiştir. IL8/
CXCL8 rs4073 gen polimorfizminin genotiplenmesi, polimeraz zincir reaksiyonu bazlı restriksiyon fragman uzunluk polimorfizmi
(PCR-RFLP) yöntemi kullanılarak belirlenmiştir. GDM hastalarında ve kontrollerde her bir gen polimorfizminin genotip sıklığını
karşılaştırmak için χ2 testi kullanıldı.
Bulgular: Gestasyonel diyabetli gebelerle sağlıklı gebeler arasında rs4073 polimorfizmi genotip ve allel frekans dağılımları açısından
istatistiksel olarak anlamlı bir farklılık saptanmamıştır (sırasıyla; p=0,260, p=1,000).
Sonuç: Bulgularımız IL8/CXCL8 rs4073 gen polimorfizminin GDM’ye yatkınlıkla ilişkili olmadığını göstermekle birlikte bulgularımızın
doğrulanması için farklı etnik grupları içeren geniş örneklem büyüklükleriyle, katılımcıların vücut kütle indeksleri, gebelik öncesi
kiloları gibi klinik parametreler de dahil edilerek yapılacak ileri çalışmalara ihtiyaç vardır

Anahtar Kelimeler

Gestasyonel Diyabet, IL-8, CXCL8, rs4073, gen polimorfizm

Investigation of the Relationship Between Gestational Diabetes Mellitus and IL8/CXCL8 rs4073 Gene Polymorphism

Öz

Aim: Gestational Diabetes (GDM) is glucose intolerance that is first diagnosed during pregnancy. Many risk factors such as advanced
gestational age, multiparity, history of GDM in a previous pregnancy, and genetic factors have been defined for GDM, which increases
the risk of complications in both mother and baby. In addition, some inflammatory mediators, chemokines, and polymorphisms of the
genes encoding them are associated with GDM. In this study, we aimed to determine the relationship between GDM susceptibility and
IL8/CXCL8 rs4073 (-251A/T) gene polymorphism by comparing the genotype and allele frequency distributions in pregnant women
diagnosed with GDM and healthy pregnant women.
Material and Methods: One hundred pregnant patients diagnosed with GDM and 100 healthy pregnant as the control group, were
included in our study. All participants were followed up in Zonguldak Bülent Ecevit University Medical Faculty Hospital, Department of Obstetrics and Gynecology. Genotyping of the IL8/CXCL8 rs4073 gene polymorphism was determined using the polymerase chain
reaction-based restriction fragment length polymorphism (PCR-RFLP) method. The χ2 test was used to compare the genotype frequency
of each gene polymorphism in GDM patients and controls.
Results: There was no statistically significant difference between pregnant women with GDM and healthy pregnant women in terms of
rs4073 polymorphism genotype and allele frequency distributions (p=0.260, p=1.000, respectively).
Conclusion: Although our findings show that IL8/CXCL8 rs4073 gene polymorphism is not associated with susceptibility to GDM,
further studies with large sample sizes including different ethnic groups and clinical parameters of participants such as body mass index
and pre-pregnancy weight, are needed to confirm our findings

Anahtar Kelimeler

Gestational diabetes mellitus, IL-8, CXCL8, rs4073, gene polymorphism

Kaynakça

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