Lectin Staining of Extensor Digitorum Longus Muscle Cell Membranes in Alloxan Diabetic Rats

Öz

Aim: Effect of alloxan-diabet on Extensor digitorum longus (EDL) skeletal muscle of rats were observed by lectin staining techniques in light microscope.

Material and Methods: After 30 days alloxan (dose of alloxan: 55 mg/kg) injected by intravenously, samples of muscles were obtained from control and diabetic rats. The muscle samples sections were cutted by cryostat microtome and stained by four biotinylated lectins [Wheat Germ Agglutinin (WGA), Pea Nut Agglutinin (PNA), Concavalin A (ConA), Griffonia simplicifolia I (GS)]. The lectins were fixed by avidin-peroxidase complex. All lectins were bounded to EDL muscle cell membranes of control and diabetic rats.

Results: GS and WGA lectins were strongly (+++) stained extensor digitorum muscle cell membranes of alloxan-diabetic rats. Not only cell membranes but also cytoplasmic miyofibrills of diabetic muscle cells were stained by GS lectin. PNA was moderate (++) stained diabetic muscle cell membranes. Con A was weakly (+) stained diabetic muscle cell membranes with respect to control cell membranes. According to our findings, alloxan-diabetes altered the molecular structure of glycoproteins in cell membranes of EDL skeletal muscles of rats.

Conclusion: We suggested that this study will contribute to diabetes research to show the damaging effects of diabetes on cell membranes.

Anahtar Kelimeler

• Extensor digitorum longus muscle cell membrane,Lectin stainning,Diabetes mellitus,Alloxan

Alloxan Diyabetli Sıçanlarda Extensor Digitorum Longus Kas Hücresi Membranlarının Lektin Boyaması

Öz

Amaç: Alloksan diyabetin sıçanların Extensor Digitorum Longus (EDL) iskelet kasına etkisi lektin boyama tekniği ile ışık mikroskobunda incelendi.

Gereç ve Yöntemler: İntravenöz yoldan alloksan enjekte edildikten 30 gün sonra, kontrol ve diyabetik grubu sıçanlardan kas örnekleri alındı. Kas örneklerinden kryostatlı mikrotom ile kesitleri alındı ve dört biyotinli lektin [Wheat Germ Agglutinin (WGA), Pea Nut Agglutinin (PNA), Concavalin A (ConA), Griffonia simplicifolia (GS)] ile boyandı. Lektinler, avidin-peroksidaz kompleksi ile tespit edildi. Lektinlerin hepsi kontrol ve diyabetik sıçan grubunun EDL iskelet kasına bağlandı.

Bulgular: GS ve WGA lektinleri alloksan diyabetik sıçanların EDL iskelet kaslarını kuvvetli (+++) boyadı. Özellikle GS lektini sadece hücre zarına değil aynı zamanda kas fibrillerini kuvvetli boyadı. PNA lektini orta derecede (++) EDL kaslarını boyadı. Con A ise kontrol grubuna göre diyabetik kasları daha zayıf (+) boyadı. Elde ettiğimiz bulgulara göre alloksan-diyabet sıçanların EDL iskelet kaslarının hücre zarlarındaki glikoproteinlerin moleküler yapısını değiştirmiştir.

Sonuç: Bu çalışma diyabetin hücre zarlarına hasar verici etkisini göstermek açısından diyabetle yapılan araştırmalara katkıda bulunacaktır.

Anahtar Kelimeler

• Extensor digitorum longus iskelet kası hücre zarı,Lektin boyama,Diabetes mellitus,Alloksan

Kaynakça

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