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An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models

Yıl 2021, Cilt: 5 Sayı: 3, 241 - 247, 30.12.2021
https://doi.org/10.25048/tudod.972167

Öz

Aim: This study aimed to evaluate the potential effect of curcumin on glucose homeostasis, insulin resistance, inflammation, and
oxidative stress in a fructose-induced metabolic syndrome rat model.
Material and Methods: 24 male adult Wistar albino rats were randomly separated in to 4 groups: control (Group 1), 20% fructose
(Group 2), 20% fructose and 100 mg/kg curcumin (Group 3), and 20% fructose, and 200 mg/kg curcumin (Group 4). Serum glucose,
insulin, and plasma lipoprotein levels were determined by an auto-analyzer. Other parameters were determined by the Elisa Assay
Method. Mann-Whitney U test was performed using the Bonferroni Correction to determine the significance of the difference between
the two group (p≤0.008). Spearman Correlation Analysis was performed among parameters.
Results: Metabolic Syndrome (MetS) was successfully created by observing increased serum glucose, high blood pressure, insulin
resistance, and dyslipidemia in Group 2 compared to Group 1 (p≤0.008). The significance of serum total antioxidant capacity (TAC)
levels among the groups could not be determined. While serum total oxidant status (TOS) and oxidative stress index (OSI) levels of
Group 2 increased significantly compared to Group 1, and the levels of these parameters were significantly decreased in Group 3 and
Group 4 compared to Group 2 (p≤0.008). The serum tumor necrosis factor-α (TNF-α) level of Group 2 increased significantly compared
to Group 1 (p≤0.008). It was determined that the serum TNF-α level of Group 3 and Group 4 decreased significantly compared to Group
2 (p≤0.008). There was a positive correlation between serum OSI and TNF-α levels and serum HOMA-IR and TNF-α levels (p≤0.01).
Conclusion: Our findings indicated that curcumin has a healing effect against MetS in the rat experimental animal model.

Destekleyen Kurum

Gazi University Scientific Research Projects

Proje Numarası

01/2019-16

Kaynakça

  • 1. Basciano H, Federico L, Adeli K. Fructose, insulin resistance, and metabolic dyslipidemia. Nutr Metab (Lond). 2005;2(1):5.
  • 2. Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Zachariah B. Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: Potential role of serine kinases. Chem Biol Interact. 2016;244:187-194.
  • 3. Roberts CK, Sindhu KK. Oxidative stress and metabolic syndrome. Life Sci. 2009;84(21-22):705-712.
  • 4. Kelany ME, Hakami TM, Omar AH. Curcumin improves the metabolic syndrome in high-fructose-diet-fed rats: role of TNF-α, NF-κB, and oxidative stress. Can J Physiol Pharmacol. 2017;95(2):140-150.
  • 5. Aggarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res. 2003;23(1A):363-398.
  • 6. Lestari ML, Indrayanto G. Curcumin. Profiles Drug Subst Excip Relat Methodol. 2014;39:113-204.
  • 7. Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol. 2009;41(1):40-59.
  • 8. Panahi Y, Hosseini MS, Khalili N, Naimi E, Simental-Mendía LE, Majeed M, Sahebkar A. Effects of curcumin on serum cytokine concentrations in subjects with metabolic syndrome: A post-hoc analysis of a randomized controlled trial. Biomed Pharmacother. 2016;82:578-582.
  • 9. Kuptniratsaikul V, Dajpratham P, Taechaarpornkul W, Buntragulpoontawee M, Lukkanapichonchut P, Chootip C, Saengsuwan J, Tantayakom K, Laongpech S. Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis: a multicenter study. Clin Interv Aging. 2014;9:451-458.
  • 10. Mazzolani F, Togni S. Oral administration of a curcumin-phospholipid delivery system for the treatment of central serous chorioretinopathy: a 12-month follow-up study. Clin Ophthalmol. 2013;7:939-945.
  • 11. Allegri P, Mastromarino A, Neri P. Management of chronic anterior uveitis relapses: efficacy of oral phospholipidic curcumin treatment. Long-term follow-up. Clin Ophthalmol. 2010;4:1201-1206.
  • 12. Milani A, Basirnejad M, Shahbazi S, Bolhassani A. Carotenoids: biochemistry, pharmacology and treatment. Br J Pharmacol. 2017;174(11):1290-1324.
  • 13. Perrone D, Ardito F, Giannatempo G, Dioguardi M, Troiano G, Lo Russo L, DE Lillo A, Laino L, Lo Muzio L. Biological and therapeutic activities, and anticancer properties of curcumin. Exp Ther Med. 2015;10(5):1615-1623.
  • 14. Stanic Z. Electrochemical investigation of some biological important compounds correlated to curcumin. In Curcumin: biosynthesis, medicinal uses and health benefits 10th Edition, New York, Nova Science Publisher, 2012
  • 15. Ferguson JJA, Stojanovski E, MacDonald-Wicks L, Garg ML. Curcumin potentiates cholesterol-lowering effects of phytosterols in hypercholesterolaemic individuals. A randomised controlled trial. Metabolism. 2018;82:22-35.
  • 16. Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Zachariah B. Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: Potential role of serine kinases. Chem Biol Interact. 2016 25;244:187-194.
  • 17. Alberti KG, Zimmet P, Shaw J; IDF Epidemiology Task Force Consensus Group. The metabolic syndrome--a new worldwide definition. Lancet. 2005;366(9491):1059-1062.
  • 18. Pan Y, Zhao D, Yu N, An T, Miao J, Mo F, Gu Y, Zhang D, Gao S, Jiang G. Curcumin improves glycolipid metabolism through regulating peroxisome proliferator activated receptor γ signalling pathway in high-fat diet-induced obese mice and 3T3-L1 adipocytes. R Soc Open Sci. 2017;4(11):170917.
  • 19. Yao Y, Wang W, Li M, Ren H, Chen C, Wang J, Wang WE, Yang J, Zeng C. Curcumin Exerts its Anti-hypertensive Effect by Down-regulating the AT1 Receptor in Vascular Smooth Muscle Cells. Sci Rep. 2016;6:25579.
  • 20. Bernardis LL, Patterson BD. Correlation between 'Lee index' and carcass fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol. 1968;40(4):527-528.
  • 21. Xydakis AM, Case CC, Jones PH, Hoogeveen RC, Liu MY, Smith EO, Nelson KW, Ballantyne CM. Adiponectin, inflammation, and the expression of the metabolic syndrome in obese individuals: the impact of rapid weight loss through caloric restriction. J Clin Endocrinol Metab. 2004;89(6):2697-2703.
  • 22. Engin A. The Definition and Prevalence of Obesity and Metabolic Syndrome. Adv Exp Med Biol. 2017;960:1-17.
  • 23. Welty FK, Alfaddagh A, Elajami TK. Targeting inflammation in metabolic syndrome. Transl Res. 2016;167(1):257-280.
  • 24. Nakagawa T, Hu H, Zharikov S, Tuttle KR, Short RA, Glushakova O, Ouyang X, Feig DI, Block ER, Herrera-Acosta J, Patel JM, Johnson RJ. A causal role for uric acid in fructose-induced metabolic syndrome. Am J Physiol Renal Physiol. 2006;290(3):F625-F631.
  • 25. Kantar Ş, Türközkan N, Bircan FS, Paşaoğlu ÖT. Beneficial effects of melatonin on serum nitric oxide, homocysteine, and ADMA levels in fructose-fed rats. Pharm Biol. 2015;53(7):1035-1041.
  • 26. Yilmaz Demirtas C, Bircan FS, Pasaoglu OT, Turkozkan N. The effects of resveratrol on hepatic oxidative stress in metabolic syndrome model induced by high fructose diet. Bratisl Lek Listy. 2018;119(1):36-40.
  • 27. Pasaoglu OT, Bircan FS, Topal T, Turkozkan N. Positive Effects of Melatonin on Renal Nitric Oxide-Asymmetric Dimethylarginine Metabolism in Fructose-Fed Rats. Metab Syndr Relat Disord. 2021;19(3):120-126.
  • 28. Kohli K, Ali J, Ansari MJ, Raheman Z. Curcumin: a natural antiinflammatory agent. Indian Journal of Pharmacology, 2005;37(3):141.
  • 29. Maithili Karpaga Selvi N, Sridhar MG, Swaminathan RP, Sripradha R. Curcumin Attenuates Oxidative Stress and Activation of Redox-Sensitive Kinases in High Fructose- and High-Fat-Fed Male Wistar Rats. Sci Pharm. 2014;83(1):159-175.
  • 30. Naik SR, Thakare VN, Patil SR. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats: evidence of its antioxidant property. Exp Toxicol Pathol. 2011;63(5):419-431.
  • 31. Hismiogullari AA, Hismiogullari SE, Karaca O, Sunay FB, Paksoy S, Can M, Yavuz O. The protective effect of curcumin administration on carbon tetrachloride (CCl 4)-induced nephrotoxicity in rats. Pharmacological Reports. 2015:67(3);410-416.
  • 32. Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Sripradha R, Badhe B. Curcumin inhibits hyperlipidemia and hepatic fat accumulation in high-fructose-fed male Wistar rats. Pharm Biol. 2016;54(12):2857-2863.
  • 33. Wu J, Li Q, Wang X, Yu S, Li L, Wu X, Chen Y, Zhao J, Zhao Y. Neuroprotection by curcumin in ischemic brain injury involves the Akt/Nrf2 pathway. PLoS One. 2013;8(3):e59843.
  • 34. Li Y, Li J, Li S, Li Y, Wang X, Liu B, Fu Q, Ma S. Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK. Toxicol Appl Pharmacol. 2015;286(1):53-63.
  • 35. Kahn CR, Wang G, Lee KY. Altered adipose tissue and adipocyte function in the pathogenesis of metabolic syndrome. J Clin Invest. 2019;129(10):3990-4000.
  • 36. Alipourfard I, Datukishvili N, Mikeladze D. TNF-α Downregulation Modifies Insulin Receptor Substrate 1 (IRS-1) in Metabolic Signaling of Diabetic Insulin-Resistant Hepatocytes. Mediators Inflamm. 2019;2019:3560819.

Kurkuminin Fruktoza İndüklenmiş Metabolik Sendromlu Sıçan Modelleri Üzerindeki Etkisinin İncelenmesi

Yıl 2021, Cilt: 5 Sayı: 3, 241 - 247, 30.12.2021
https://doi.org/10.25048/tudod.972167

Öz

Amaç: Çalışmamızda, fruktoza bağlı metabolik sendrom sıçan modelinde kurkuminin glukoz homeostazı, insülin direnci, inflamasyon
ve oksidatif stres üzerindeki olası etkilerinin değerlendirmesini amaçladık.
Gereç ve Yöntemler: 24 adet erkek erişkin Wistar albino rat rastgele 4 gruba ayrıldı: kontrol (Grup 1), %20 fruktoz (Grup 2), %20
fruktoz ve 100 mg/kg kurkumin (Grup 3) ve %20 fruktoz, ve 200 mg/kg kurkumin (Grup 4). Serum glukoz, insülin ve plazma lipoprotein
seviyeleri otoanalizör tarafından belirlenmiştir. Diğer parametreler ise Elisa Assay Metodu ile incelenmiştir. İki grup arasındaki farkın
anlamlılığını belirlemek için Mann-Whitney U testi kullanılarak Bonferroni Düzeltmesi yapılmıştır (p≤0,008). Parametreler arasında ise
Spearman Korelasyon Analizi yapılmıştır Bulgular: Grup 2'de Grup 1'e göre artan serum glukozu, yüksek tansiyon, insülin direnci ve dislipidemi gözlenerek Metabolik Sendrom
(MetS) başarıyla oluşturulmuştur (p≤0,008). Gruplar arasında serum total antioksidan kapasite (TAC) düzeylerinde farkın anlamlılığı
bulunamamıştır. Grup 2'nin serum total oksidan durum (TOS) ve oksidatif stres indeksi (OSI) seviyeleri Grup 1'e göre anlamlı olarak
artarken, Grup 3 ve Grup 4'te Grup 2'ye göre bu parametrelerin düzeyleri anlamlı olarak azalmıştır (p≤0,008). Grup 2'nin serum tümör
nekrozis faktör-α (TNF-α) düzeyi Grup 1'e göre anlamlı düzeyde artmıştır (p≤0,008). Grup 3 ve Grup 4'ün serum TNF-α düzeyinin
Grup 2'ye göre anlamlı düzeyde düştüğü belirlenmiştir (p≤ 0,008). Serum OSI ve TNF-α düzeyleri ile serum HOMA-IR ve TNF-α
düzeyleri arasında pozitif korelasyon saptanmıştır (p≤0,01).
Sonuç: Bulgularımız, sıçan deneysel hayvan modelinde kurkuminin MetS'e karşı iyileştirici bir etkiye sahip olduğunu göstermiştir

Proje Numarası

01/2019-16

Kaynakça

  • 1. Basciano H, Federico L, Adeli K. Fructose, insulin resistance, and metabolic dyslipidemia. Nutr Metab (Lond). 2005;2(1):5.
  • 2. Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Zachariah B. Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: Potential role of serine kinases. Chem Biol Interact. 2016;244:187-194.
  • 3. Roberts CK, Sindhu KK. Oxidative stress and metabolic syndrome. Life Sci. 2009;84(21-22):705-712.
  • 4. Kelany ME, Hakami TM, Omar AH. Curcumin improves the metabolic syndrome in high-fructose-diet-fed rats: role of TNF-α, NF-κB, and oxidative stress. Can J Physiol Pharmacol. 2017;95(2):140-150.
  • 5. Aggarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res. 2003;23(1A):363-398.
  • 6. Lestari ML, Indrayanto G. Curcumin. Profiles Drug Subst Excip Relat Methodol. 2014;39:113-204.
  • 7. Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol. 2009;41(1):40-59.
  • 8. Panahi Y, Hosseini MS, Khalili N, Naimi E, Simental-Mendía LE, Majeed M, Sahebkar A. Effects of curcumin on serum cytokine concentrations in subjects with metabolic syndrome: A post-hoc analysis of a randomized controlled trial. Biomed Pharmacother. 2016;82:578-582.
  • 9. Kuptniratsaikul V, Dajpratham P, Taechaarpornkul W, Buntragulpoontawee M, Lukkanapichonchut P, Chootip C, Saengsuwan J, Tantayakom K, Laongpech S. Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis: a multicenter study. Clin Interv Aging. 2014;9:451-458.
  • 10. Mazzolani F, Togni S. Oral administration of a curcumin-phospholipid delivery system for the treatment of central serous chorioretinopathy: a 12-month follow-up study. Clin Ophthalmol. 2013;7:939-945.
  • 11. Allegri P, Mastromarino A, Neri P. Management of chronic anterior uveitis relapses: efficacy of oral phospholipidic curcumin treatment. Long-term follow-up. Clin Ophthalmol. 2010;4:1201-1206.
  • 12. Milani A, Basirnejad M, Shahbazi S, Bolhassani A. Carotenoids: biochemistry, pharmacology and treatment. Br J Pharmacol. 2017;174(11):1290-1324.
  • 13. Perrone D, Ardito F, Giannatempo G, Dioguardi M, Troiano G, Lo Russo L, DE Lillo A, Laino L, Lo Muzio L. Biological and therapeutic activities, and anticancer properties of curcumin. Exp Ther Med. 2015;10(5):1615-1623.
  • 14. Stanic Z. Electrochemical investigation of some biological important compounds correlated to curcumin. In Curcumin: biosynthesis, medicinal uses and health benefits 10th Edition, New York, Nova Science Publisher, 2012
  • 15. Ferguson JJA, Stojanovski E, MacDonald-Wicks L, Garg ML. Curcumin potentiates cholesterol-lowering effects of phytosterols in hypercholesterolaemic individuals. A randomised controlled trial. Metabolism. 2018;82:22-35.
  • 16. Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Zachariah B. Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: Potential role of serine kinases. Chem Biol Interact. 2016 25;244:187-194.
  • 17. Alberti KG, Zimmet P, Shaw J; IDF Epidemiology Task Force Consensus Group. The metabolic syndrome--a new worldwide definition. Lancet. 2005;366(9491):1059-1062.
  • 18. Pan Y, Zhao D, Yu N, An T, Miao J, Mo F, Gu Y, Zhang D, Gao S, Jiang G. Curcumin improves glycolipid metabolism through regulating peroxisome proliferator activated receptor γ signalling pathway in high-fat diet-induced obese mice and 3T3-L1 adipocytes. R Soc Open Sci. 2017;4(11):170917.
  • 19. Yao Y, Wang W, Li M, Ren H, Chen C, Wang J, Wang WE, Yang J, Zeng C. Curcumin Exerts its Anti-hypertensive Effect by Down-regulating the AT1 Receptor in Vascular Smooth Muscle Cells. Sci Rep. 2016;6:25579.
  • 20. Bernardis LL, Patterson BD. Correlation between 'Lee index' and carcass fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol. 1968;40(4):527-528.
  • 21. Xydakis AM, Case CC, Jones PH, Hoogeveen RC, Liu MY, Smith EO, Nelson KW, Ballantyne CM. Adiponectin, inflammation, and the expression of the metabolic syndrome in obese individuals: the impact of rapid weight loss through caloric restriction. J Clin Endocrinol Metab. 2004;89(6):2697-2703.
  • 22. Engin A. The Definition and Prevalence of Obesity and Metabolic Syndrome. Adv Exp Med Biol. 2017;960:1-17.
  • 23. Welty FK, Alfaddagh A, Elajami TK. Targeting inflammation in metabolic syndrome. Transl Res. 2016;167(1):257-280.
  • 24. Nakagawa T, Hu H, Zharikov S, Tuttle KR, Short RA, Glushakova O, Ouyang X, Feig DI, Block ER, Herrera-Acosta J, Patel JM, Johnson RJ. A causal role for uric acid in fructose-induced metabolic syndrome. Am J Physiol Renal Physiol. 2006;290(3):F625-F631.
  • 25. Kantar Ş, Türközkan N, Bircan FS, Paşaoğlu ÖT. Beneficial effects of melatonin on serum nitric oxide, homocysteine, and ADMA levels in fructose-fed rats. Pharm Biol. 2015;53(7):1035-1041.
  • 26. Yilmaz Demirtas C, Bircan FS, Pasaoglu OT, Turkozkan N. The effects of resveratrol on hepatic oxidative stress in metabolic syndrome model induced by high fructose diet. Bratisl Lek Listy. 2018;119(1):36-40.
  • 27. Pasaoglu OT, Bircan FS, Topal T, Turkozkan N. Positive Effects of Melatonin on Renal Nitric Oxide-Asymmetric Dimethylarginine Metabolism in Fructose-Fed Rats. Metab Syndr Relat Disord. 2021;19(3):120-126.
  • 28. Kohli K, Ali J, Ansari MJ, Raheman Z. Curcumin: a natural antiinflammatory agent. Indian Journal of Pharmacology, 2005;37(3):141.
  • 29. Maithili Karpaga Selvi N, Sridhar MG, Swaminathan RP, Sripradha R. Curcumin Attenuates Oxidative Stress and Activation of Redox-Sensitive Kinases in High Fructose- and High-Fat-Fed Male Wistar Rats. Sci Pharm. 2014;83(1):159-175.
  • 30. Naik SR, Thakare VN, Patil SR. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats: evidence of its antioxidant property. Exp Toxicol Pathol. 2011;63(5):419-431.
  • 31. Hismiogullari AA, Hismiogullari SE, Karaca O, Sunay FB, Paksoy S, Can M, Yavuz O. The protective effect of curcumin administration on carbon tetrachloride (CCl 4)-induced nephrotoxicity in rats. Pharmacological Reports. 2015:67(3);410-416.
  • 32. Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Sripradha R, Badhe B. Curcumin inhibits hyperlipidemia and hepatic fat accumulation in high-fructose-fed male Wistar rats. Pharm Biol. 2016;54(12):2857-2863.
  • 33. Wu J, Li Q, Wang X, Yu S, Li L, Wu X, Chen Y, Zhao J, Zhao Y. Neuroprotection by curcumin in ischemic brain injury involves the Akt/Nrf2 pathway. PLoS One. 2013;8(3):e59843.
  • 34. Li Y, Li J, Li S, Li Y, Wang X, Liu B, Fu Q, Ma S. Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK. Toxicol Appl Pharmacol. 2015;286(1):53-63.
  • 35. Kahn CR, Wang G, Lee KY. Altered adipose tissue and adipocyte function in the pathogenesis of metabolic syndrome. J Clin Invest. 2019;129(10):3990-4000.
  • 36. Alipourfard I, Datukishvili N, Mikeladze D. TNF-α Downregulation Modifies Insulin Receptor Substrate 1 (IRS-1) in Metabolic Signaling of Diabetic Insulin-Resistant Hepatocytes. Mediators Inflamm. 2019;2019:3560819.
Toplam 36 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makalesi
Yazarlar

Suzan Muratoğlu Severcan 0000-0001-5978-8144

Gülce Koca 0000-0002-2646-1003

Çınar Severcan 0000-0002-3806-6406

Canan Yılmaz 0000-0002-6799-6522

Özge Paşaoğlu 0000-0002-5221-9034

Hatice Pasaoglu 0000-0001-8343-7432

Proje Numarası 01/2019-16
Yayımlanma Tarihi 30 Aralık 2021
Kabul Tarihi 6 Aralık 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 5 Sayı: 3

Kaynak Göster

APA Muratoğlu Severcan, S., Koca, G., Severcan, Ç., Yılmaz, C., vd. (2021). An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models. Turkish Journal of Diabetes and Obesity, 5(3), 241-247. https://doi.org/10.25048/tudod.972167
AMA Muratoğlu Severcan S, Koca G, Severcan Ç, Yılmaz C, Paşaoğlu Ö, Pasaoglu H. An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models. Turk J Diab Obes. Aralık 2021;5(3):241-247. doi:10.25048/tudod.972167
Chicago Muratoğlu Severcan, Suzan, Gülce Koca, Çınar Severcan, Canan Yılmaz, Özge Paşaoğlu, ve Hatice Pasaoglu. “An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models”. Turkish Journal of Diabetes and Obesity 5, sy. 3 (Aralık 2021): 241-47. https://doi.org/10.25048/tudod.972167.
EndNote Muratoğlu Severcan S, Koca G, Severcan Ç, Yılmaz C, Paşaoğlu Ö, Pasaoglu H (01 Aralık 2021) An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models. Turkish Journal of Diabetes and Obesity 5 3 241–247.
IEEE S. Muratoğlu Severcan, G. Koca, Ç. Severcan, C. Yılmaz, Ö. Paşaoğlu, ve H. Pasaoglu, “An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models”, Turk J Diab Obes, c. 5, sy. 3, ss. 241–247, 2021, doi: 10.25048/tudod.972167.
ISNAD Muratoğlu Severcan, Suzan vd. “An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models”. Turkish Journal of Diabetes and Obesity 5/3 (Aralık 2021), 241-247. https://doi.org/10.25048/tudod.972167.
JAMA Muratoğlu Severcan S, Koca G, Severcan Ç, Yılmaz C, Paşaoğlu Ö, Pasaoglu H. An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models. Turk J Diab Obes. 2021;5:241–247.
MLA Muratoğlu Severcan, Suzan vd. “An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models”. Turkish Journal of Diabetes and Obesity, c. 5, sy. 3, 2021, ss. 241-7, doi:10.25048/tudod.972167.
Vancouver Muratoğlu Severcan S, Koca G, Severcan Ç, Yılmaz C, Paşaoğlu Ö, Pasaoglu H. An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models. Turk J Diab Obes. 2021;5(3):241-7.

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