DETERMINATION OF THE EFFECTS OF BISPHENOL A (BPA) ON TOTAL HEMOCYTE COUNTS IN FRESHWATER MUSSELS (Unio mancus)

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 157 - 157, 16.02.2017

Feriha Yıldırım Leila Mehrnıa Pınar Aslan Kevser Yılmaz A.Çağlan Karasu Benlı Figen Erkoç

Öz

Bisphenol A
(BPA), is a synthetical chemical that is widely used in polycarbonate
plasticsandchemical adhesive resins. There are many studies about that the
development and reproduction system negatively affects in various vertebrate
and invertebrate organisms by this chemical which has effects of endocrine
disruptors identified. However, in the determination of the amount of damage
that is still uncertainty and continues research. In this study, total number
of hemocytes were examined to determine the potential damage of BPA on the
reproduction and body systems of Unio mancus eucirrus Bourguignat, 1860 (Bivalvia)
which is a species of freshwater mussels.

The mussels,
that have been collected from Sinop-Karasu stream (average lenght 55,2±3,46 mm
and average weight 21,97±4,30 g), are carried out in 15 liter tanks by putting
15 mussels in each, after 15 days adaptation in dechlorinated tap water
supplied in the laboratory. They were exposed to four different sublethal BPA
doses (0.1 mg/L¸ 1 mg/L¸ 5 mg/L ve 10 mg/L) and at the end of 144 hours
hemolymph samples are taken and total hemocytes are counted with a light microscope
through the hemocytometer. Statistical analysis performed by Kruskal-Wallis
test was used.

It has been
observed a significantincrease on the total number of hemocytes of U. mancus
which are exposed to BPA in comparison to the control group (p <0.05).
Accordingly, the determination of the total hemocyte counts infreshwater
musselswas found to be a good biomarkerto examine the effects of BPA that is
endocrine disruptor, in freshwater ecosystems.

Anahtar Kelimeler

DETERMINATION OF THE EFFECTS OF BISPHENOL A (BPA) ON TOTAL HEMOCYTE COUNTS IN FRESHWATER MUSSELS (Unio mancus)

Kaynakça

• Feriha YILDIRIM, Leila MEHRNIA, Pınar ASLAN, Kevser YILMAZ, A.Çağlan KARASU BENLI, Figen ERKOÇ
• Gazi Üniversitesi, Teknikokullar, Ankara, 06500 Ankara Üniversitesi, Ankara, 06500