HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) OVARY TISSUE

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 205 - 205, 16.02.2017

Nazan Deniz Yön Ceyda Öztürk Mustafa Zengin Cansu Akbulut

Öz

Examination the histopathological
effects of (4s)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic acid on
ovary tissue of zebrafish were aimed.

 (4S)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid is new synthesized substance which obtained from
cysteine and valine. Because of thiazolidine derivates have important
biological responses scientist work intensively on these compounds recent
years. It is obvious that thiazolidine contained compounds will be used in
future in the pharmaceutical industry to treat important diseases.

Zebrafish were raised in a
computer-controlled incubation chamber, and received 14 hours of daylight and
10 hours of darkness everyday. After one week adaptation period zebrafish
divided into four group (n=10) as one control and experimental groups (0,2mM, 0,4mM,
0,6Mm). For investigating the effects of (4s)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid , ovary tissues were dissected after 5 day of
the exposure. Tissues were fixed with 10% neutral buffered formalin and
dehydration were carried out in an ascending series of ethanol. After tissues
were cleared in xylene, embedded in paraffin wax and cut into 5 µm sections on
a microtome. The sections were stained with hematoxylin (H&E). Results were
evaluated with light microscope.

In control group normal ovary
histology was observed. Primary oocytes, cortical alveolus stage oocytes,
vitellogenic oocytes and mature oocytes were monitored clearly. In 0,2mM
exposure group, seperation between follicular epithelium and zona radiata was
also monitored at cortical alveoli. Opening at structure of oocytes were
observed. Openings between zona radiata and vitelline envelope were detected at
cortical alveoli. Disintegration of vitelline envelope were monitored at
vitellogenic oocyte structure. Vacuolization were observed at cortical alveolus
stage oocyte structure. In 0,4mM exposure group, seperation between follicular
epithelium and zona radiata was also monitored at mature oocyte. Vacuolization
at cortical alveolus stage oocytes were monitored. Seperation between
follicular epithelium and zona radiata was monitored at cortical alveoli. In
0,6mM exposure group, openings between zona radiata and vitelline envelope were
detected at cortical alveoli. Vacuolization at cortical alveolus stage oocytes
were monitored. Degeneration was detected at mature oocyte.

Anahtar Kelimeler

HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) OVARY TISSUE

Kaynakça

• Nazan Deniz YÖN, Ceyda ÖZTÜRK, Mustafa ZENGİN, Cansu AKBULUT
• Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Turkey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Turkey