HISTOPATHOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THİAZOLIDINE-4-CARBOXYLIC ACID ON INTESTINE TISSUE OF ZEBRAFISH (Danio rerio)

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 294 - 294, 16.02.2017

Cansu Akbulut Büşra Fidan Mustafa Zengin Nazan Deniz Yön

Öz

Investigation
the histopathological effects of (4s)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid on intestine tissue of zebrafish were aimed.

 (4S)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic
acid is new synthesized substance which obtained from cysteine and valine.
Because of thiazolidine derivates have important biological responses scientist
work intensively on these compounds recent years. It is obvious that thiazolidine
contained compounds will be used in future in the pharmaceutical industry to
treat important diseases.

After one week
adaptation period zebrafish divided into four group (n=10) as one control and 3
experimental groups (0.2 mM,0.4mM 0.6mM). Intestine were dissected after 5 day
of the exposure. Tissues were fixed with 10% neutral buffered formalin and
dehydration were carried out in an ascending series of ethanol. Tissues were
cleared in xylene, embedded in paraffin wax and cut into 5 µm sections on a microtome.
The sections were stained with hematoxylin(H&E). Results were evaluated
with light microscope.

In control group
normal intestine histology have been observed. In 0.2 mM degeneration and
fusion at villi structures and inflammation at submucosa were detected. In 0.4
mM thiazolidine exposed group, degeneration and vacuolization at villi
structure and hypertrophy at serosa were observed. In 0.6 mM thiazolidine
exposed group, degeneration at vili structure, hypertrophy at muscularis
externa, vacuolization at submucosa and increase in the number of goblet cells
were observed.

Anahtar Kelimeler

HISTOPATHOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THİAZOLIDINE-4-CARBOXYLIC ACID ON INTESTINE TISSUE OF ZEBRAFISH (Danio rerio)

Kaynakça

• Cansu AKBULUT, Büşra FİDAN, Mustafa ZENGİN, Nazan Deniz YÖN
• Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Turkey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Turkey