FAST DETECTION OF ACETYLSALICYLIC ACID BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY(LC-MSMS)

Yıl 2017, Volume 2, Issue 1(4), 30 - 30, 01.11.2017

Sedat Abusoglu Ali Unlu Abdullah Sivrikaya

Öz

Objectives

Acetylsalicylic acid (ASA) is the
most widely used as an analgesic, anti-inflammatory and antipyretic drug, and
also used to inhibit cyclooxygenase dependent platelet aggregation.   The aim
of this study was to develop a simple, fast and accurate tandem mass method for
determination and quantification of ASA.

 

Methods

Chromatographic seperation was performed
using an Shimadzu LC-20-AD (Kyoto, Japan) coupled
with a ABSCIEX API 3200 triple quadrupole massspectrometer(USA)
equipped with an electrospray ion source (ESI)
operating in negative mode.

 

Results

Intraassay CV values for acetylsalicylic
acid were 8% and 6% for 0.8 and 100 µg/L, respectively.

 

Discussion

The narrow therapeutic range of
these drugs are of importance to be analyzed at critical concentrations. This
method has capability to determine these levels.

 

Conclusion

We can conclude that the
developed method can be useful for clinical studies and routine therapeutic
drug monitoring with the desired precision and accuracy.

 

Keywords

Salicylic toxicity, tandem mass,
high performance, chromatography.

 

Acknowledgments: No conflict
of interest

Anahtar Kelimeler

FAST DETECTION OF ACETYLSALICYLIC ACID BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY(LC-MSMS)

Kaynakça

• Sedat Abusoglu*, Ali Unlu, Abdullah Sivrikaya Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey