Impact of an Experimental Remineralizing Agent Incorporating Bioactive Glass on Enamel Hardness

Yıl 2024, , 357 - 366, 30.08.2024

İdil Gönüllü , Hande Kemaloglu , Cem Peşkersoy , Houman Golchin , Murat Turkun

https://doi.org/10.58711/turkishjdentres.vi.1507367

Öz

Aim: The aim of this in vitro study was to investigate the effect of an experimental remineralization agent containing bioactive glass on the surface hardness of enamel.
Material and Method: A total of thirty samples were prepared for our investigation. After being embedded in acrylic, these samples were randomly divided into three groups and treated with three different methods: G1. Casein phosphopeptide-amorphous calcium phosphate [GC Tooth Mousse]; G2. Experimental remineralization agent containing bioactive glass; G3. Artificial saliva solution. The samples
underwent demineralization using 37% phosphoric acid for a duration of 30 seconds prior to the application of remineralization agents. Then the agents were administered twice daily for a period of 5 minutes. Following the removal of the agents, the samples were stored in artificial saliva until the subsequent procedure. The process was repeated daily for a duration of 10 days. The surface hardness of the samples was assessed on three separate times in total: initially, following demineralization, and after remineralization, using Vickers microhardness testing.
Results: After demineralization with phosphoric acid, the groups’ microhardness values significantly decreased (p<0.05). Microhardness increased significantly in the test groups after remineralization, but not in the control group (p>0.05). When evaluating both remineralization agents, no statistically
significant disparity was observed in the microhardness increases they induced on the enamel surface.
Conclusion: Bioactive glass significantly increased demineralized enamel surface microhardness, according to the study. However, the experimental remineralizastion agent’s physical and biological properties need further study. Additionally, demineralized enamel surfaces did not become
harder with artificial saliva solution alone.

Anahtar Kelimeler

Demineralization, Microhardness, Bioactive glass, Remineralization

Biyoaktif Cam İçeren Deneysel Bir Remineralizasyon Ajanının Minenin Yüzey Sertliğine Etkisi

Öz

Amaç: Bu in vitro çalışmanın amacı, biyoaktif cam içeren deneysel bir remineralizasyon ajanının minenin yüzey sertliği üzerindeki etkisini araştırmaktır.
Gereç ve Yöntem: Onbeş adet insan 3. molar dişinden toplam otuz adet mine kesiti hazırlandı. Örnekler akrilik içine gömüldükten sonra rastgele üç gruba ayrıldı: G1. Kazein fosfopeptid-amorf kalsiyum fosfat [GC Tooth Mousse]; G2. Biyoaktif cam içeren deneysel remineralizasyon ajanı; G3. Yapay tükürük çözeltisi. Örnekler, remineralizasyon ajanlarının uygulanmasından önce 30 saniye süreyle %37’lik fosforik asit kullanılarak demineralizasyona tabi tutuldu. Daha sonra örneklerin yüzeylerine 5 dakikalık bir süre boyunca günde iki kez olmak üzere remineralizasyon ajanları uygulandı. Ajanların pamuk peletler ile temizlenmesinin ardından, örnekler bir sonraki tekrara kadar yapay bir tükürük çözeltisinde saklandı.
İşlem 10 gün boyunca günlük olarak tekrarlandı. Örneklerin yüzey sertliği Vickers mikrosertlik testi kullanılarak başlangıçta, demineralizasyonu takiben ve remineralizasyondan sonra olmak üzere üç ayrı zamanda değerlendirildi.
Bulgular: Fosforik asitle demineralizasyon sonrasında grupların mikrosertlik değerlerinde anlamlı bir azalma tespit edildi (p<0.05). Remineralizasyon ajanları test gruplarında mikrosertliği arttırırken kontrol grubunda bir artış görülmedi (p>0.05). Her iki remineralizasyon ajanı karşılaştırıldığında, mine yüzeyinde meydana getirdikleri mikrosertlik artışları arasında istatistiksel olarak anlamlı bir fark saptanmadı.
Sonuç: Biyoaktif cam içeren deneysel ajanın demineralize mine yüzeylerinin mikrosertlik değerlerini anlamlı şekilde artırdığı tespit edildi. Ancak, deneysel ajanın fiziksel ve biyolojik özelliklerinin daha ileri araştırmalarla incelenmesi gerekmektedir. Ayrıca, yapay tükürük solüsyonunun tek başına demineralize mine yüzeylerinin sertliğini artırmadığı belirlenmiştir.

Anahtar Kelimeler

demineralizasyon, mikrosertlik, biyoaktif cam, remineralizasyon

Kaynakça

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