The Role of Ozone Applications in Endodontics
Yıl 2024,
Cilt: 3 Sayı: 2, 390 - 397, 30.08.2024
Merve Gökyar
,
İdil Özden
,
Hesna Sazak Öveçoğlu
Öz
Ozone is recommended as an alternative or supportive treatment agent to current treatment methods. The prophylactic and therapeutic effects of ozone have been proven in the field of medicine. Ozone’s disinfectant, antimicrobial, and tissue healing properties have highlighted its use in dentistry. Ozone
has a very high potential for antimicrobial use in endodontics. In endodontics, ozonized water, ozonized oil, and ozone gas can be used as irrigation solutions or intracanal medicaments. Ozone can also be used for postoperative pain control in endodontics.
Kaynakça
- 1. Bocci V. Ozone as Janus: this controversial gas can be
either toxic or medically useful. Mediators Inflamm.
2004;13(1):3-11.
- 2. Saini R. Ozone therapy in dentistry: A strategic review. J
Nat Sci Biol Med. 2011;2(2):151-3.
- 3. Di Paolo N, Bocci V, Gaggioti E. Ozone therapy. Int J Artif
Organs. 2004;27(3):168-75.
- 4. Bocci VA. Scientific and medical aspects of ozone therapy.
State of the art. Arch Med Res. 2006;37(4):425-35.
- 5. Sen S, Sen S. Ozone therapy a new vista in dentistry:
integrated review. Medical Gas Research. 2020;10(4),189–
192.
- 6. Sujatha B, Kumar M, Pratap G, Vardhan R. Ozone therapy–A
paradigm shift in dentistry. Health Sci. 2013;2(3):1-10.
- 7. Azarpazhooh A, Limeback H. The application of ozone
in dentistry: a systematic review of literature. J Dent.
2008;36(2), 104–116.
- 8. McKenna DF, Borzabadi-Farahani A, Lynch E. The effect
of subgingival ozone and/or hydrogen peroxide on the
development of peri-implant mucositis: a double- blind
randomized controlled trial. Int J Oral Maxillofac Implants.
2013;28(6):1483-9.
- 9. Baumgartner JC, Cuenin PR. Efficacy of several
concentrations of sodium hypochlorite for root canal
irrigation. J Endod. 1992;18(12):605-12.
- 10. Brugnera A, Zanin F, Barbin EL, Spano JC, Santana
R, Pécora JD. Effects of Er: YAG and Nd: YAG laser
irradiation on radicular dentine permeability using different
irrigating solutions. Lasers Surg Med. 2003;33(4):256-9.
- 11. Song M, Zeng Q, Xiang Y, Gao L, Huang J, Huang J,
Wu K, Lu J. The antibacterial effect of topical ozone on
the treatment of MRSA skin infection. Mol Med Rep.
2018;17(2):2449–2455.
- 12. Tiwari S, Avinash A, Katiyar S, Aarthi Iyer A, Jain S. Dental
applications of ozone therapy: A review of literature. The
Saudi J Dent Res. 2017; 8(1-2):105- 111.
- 13. Silva EJNL, Prado MC, Soares DN, Hecksher F, Martins
JNR, Fidalgo TKS. The effect of ozone therapy in root
canal disinfection: a systematic review. Int Endod J.
2020;53(3):317-332.
- 14. Kaptan F, Güven EP, Topcuoglu N, Yazici M, Külekçi G. In
vitro assessment of the recurrent doses of topical gaseous
ozone in the removal of Enterococcus faecalis biofilms in
root canals. Niger J Clin Pract. 2014;17(5):573–578.
- 15. Polydorou O, Pelz K, Hahn P. Antibacterial effect of an
ozone device and its comparison with two dentin‐ bonding
systems. Eur J Oral Sci. 2006;114(4):349-53.
- 16. Sousa SIV, Alvim‐Ferraz MCM, Martins FG, Pereira MC.
Ozone exposure and its influence on the worsening of
childhood asthma. Allergy. 2009;64(7):1046-55.
- 17. Halbauer K, Prskalo K, Jankovic B, Tarle Z, Panduric V,
Kalenic S. Efficacy of ozone on microorganisms in the
tooth root canal. Coll Antropol. 2013;37(1):101-7.
- 18. Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA. An
in vitro evaluation of the ability of ozone to kill a strain of
Enterococcus faecalis. Int Endod J. 2005;38(1):22-9.
- 19. Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita
M, Nishihara T. Efficacy of ozone on survival and
permeability of oral microorganisms. Oral Microbiol
- 20. Thanomsub B, Anupunpisit V, Chanphetch S,
Watcharachaipong T, Poonkhum R, Srisukonth C. Effects of
ozone treatment on cell growth and ultrastructural changes
in bacteria. J Gen Appl Microbiol. 2002;48(4):193-9.
- 21. Baysan A, Whiley RA, Lynch E. Antimicrobial effect
of a novel ozone-Immunol. generating device on microorganisms
associated with primary root carious lesions in
vitro. Caries Res. 2000;34(6):498-501.
- 22. Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E,
Hollweck R, Hickel R, Brand K. Effect of ozone on oral
cells compared with established antimicrobials. Eur J Oral
Sci. 2006;114(5):435-40.
- 23. Bal FA, Ozkocak I, Cadirci BH, Karaarslan ES,
Cakdinleyen M, Agaccioglu M. Effects of photodynamic
therapy with indocyanine green on Streptococcus mutans
biofilm. Photodiagnosis Photodyn Ther. 2019;26:229-234.
- 24. Kapdan A, Öztaş N, Sümer Z. Comparing the antibacterial
activity of gaseous ozone and chlorhexidine solution on a
tooth cavity model. J Clin Exp Dent. 2013;5(3):e133-e137.
- 25. Pinheiro SL, da Silva CC, da Silva LA, Cicotti MP, da
Silveira Bueno CE, Fontana CE et al. Antimicrobial
efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine,
and ozonated water as irrigants in mesiobuccal root canals
with severe curvature of mandibular molars. Eur J Dent.
2018;12(1):94–99.
- 26. Agarwal S, Tyagi P, Deshpande A, Yadav S, Jain V, Rana
KS. Comparison of antimicrobial efficacy of aqueous ozone,
green tea, and normal saline as irrigants in pulpectomy
procedures of primary teeth. J Indian Soc Pedod Prev Dent.
2020;38(2):164-170.
- 27. Mehta N, Gupta A, Mahesh S, Abraham D, Singh A,
Jala S, Chauhan N, Sreen D. Comparative evaluation of
antibacterial efficacy of Allium sativum extract, aqueous
ozone, diode laser, and 3% sodium hypochlorite in root
canal disinfection: An in vivo study. J Conserv Dent. 2020;
23(6): 577–582.
- 28. Hubbezoglu I, Zan R, Tunc T, Sumer Z. Antibacterial
Efficacy of Aqueous Ozone in Root Canals Infected
by Enterococcus faecalis. Jundishapur J Microbiol.
2014;7(7):e11411.
- 29. Estrela C, Estrela CRA, Decurcio DA, Hollanda ACB,
Silva JA. Antimicrobial efficacy of ozonated water, gaseous
ozone, sodium hypochlorite and chlorhexidine in infected
human root canals. Int Endod J. 2007;40(2):85-93.
- 30. Moraes MM, Coelho MS, Nascimento WM, Nogales CG,
de Campos FUF, Soares AJ, Frozoni M. The antimicrobial
effect of different ozone protocols applied in severe curved
canals contaminated with Enterococcus faecalis: ex vivo
study. Odontology. 2021;109(3):696–700.
- 31. Boch T, Tennert C, Vach K, Al-Ahmad A, Hellwig E,
Polydorou O. Effect of gaseous ozone on Enterococcus
faecalis biofilm– an in vitro study. Clin Oral Investig.
2016;20(7):1733-39.
- 32. Küçük F, Yıldırım S, Çetiner S. Cytotoxicity assessment of
different doses of ozonated water on dental pulp cells. BMC
Oral Health. 2021;19;21(1):32.
- 33. Reddy KS, Prasad SD, Sirisha PD, Prashanth V. Evaluation
of Antimicrobial Efficacy of Ozonated Sesame Oil,
Calcium Hydroxide and their Combination as Intracanal
Medicament against Candida Albicans: An in-vitro study.
Adv Hum Biol. 2015;5(2):78-87.
- 34. Silveira AMV, Lopes HP, Siqueira Jr JF, Macedo SB,
Consolaro A. Periradicular repair after two-visit endodontic
treatment using two different intracanal medications
compared to single- visit endodontic treatment. Braz Dent
J. 2007;18(4):299-304.
- 35. Kriplani S, Sedani S, Mishra A, Umre U. Non-surgical
Management of Periapical Lesions With the Use of Newer
Modalities in Adjunct to the Conventional: A Case Series.
Cureus. 2024;16(3): e57314.
- 36. Vasavada K, Kapoor S. Evaluation of ozonized calcium
hydroxide as an effective intracanal medicament during
root canal procedures: an in vitro observational study. Med
Gas Res. 2020;10(3):122-124.
- 37. Khalilov R. Semptomatik Apikal Periodontitisli Molar
Dişlere Sahip Hastalarda Kanal İçi Ozon Uygulamasının
Postoperatif Ağrıya Etkisi: Randomize, Plasebo Kontrollü
Klinik Çalışma (Uzmanlık Tezi) Erzurum: Atatürk
Üniversitesi Diş Hekimliği Fakültesi, Endodonti Anabilim
Dalı;2017.
- 38. Sağlam H, Aladağ H. Comparison of ıntracanal ozone and
low‐level laser therapy on postoperative pain in vital teeth
with symptomatic apical periodontitis:placebo‐controlled
randomize trial. Lasers Med Sci. 2023; 38(1):227.
- 39. Sinha N, Asthana G, Parmar G, Langaliya A, Shah J,
Kumbhar A, Singh B. Evaluation of Ozone Therapy in
Endodontic Treatment of Teeth with Necrotic Pulp and
Apical Periodontitis: A Randomized Clinical Trial. J Endod.
2021;47(12):1820–1828.
- 40. Ahmed N, Suzuki S, Mosallam R. Effect of Oleozon on
Healing of Exposed Pulp Tissues. J Am Sci. 2011;7(5):38–
44.
- 41. Chandra SP, Chandrasekhar R, Uloopi KS, Vinay C, Kumar
NM. Success of root fillings with zinc oxide-ozonated oil
in primary molars: preliminary results. Eur Arch Paediatr
Dent. 2014;15(3):191-5.
- 42. El-Desouky SS, Omer SMM, Ghouraba RF, Ali Abdel Latif
RM, Kabbash IA, Hadwa SM. Zinc oxide-ozonated olive
oil as a new root canal filling material in primary molars:
a clinical randomized controlled trial. Clin Oral Investig.
2023;27(12):7395-7405.
- 43. Maiya A. Applications of ozone in dentistry. Int J Clin Den
Sci. 2011;2:23-7.
- 44. Menzel DB. Ozone: an overview of its toxicity in man and
animals. J Toxicol Environ Health. 1984;13(2- 3):181-204.
- 45. Pattanaik B, Jetwa D, Pattanaik S, Manglekar S, Naitam
DN, Dani A. Ozone therapy in dentistry: a literature review.
J Interdisciplinary Dent. 2011;1(2):87.
Endodontide Ozon Uygulamalarının Yeri
Yıl 2024,
Cilt: 3 Sayı: 2, 390 - 397, 30.08.2024
Merve Gökyar
,
İdil Özden
,
Hesna Sazak Öveçoğlu
Öz
Ozon, mevcut tedavi yöntemlerine bir alternatif veya yardımcı tedavi ajanı olarak tavsiye edilmektedir. Ozonun profilaktik ve tedavi edici etkisi tıp alanında kanıtlanmıştır. Ozonun sahip olduğu dezenfektan, antimikrobiyal ve doku iyileştirici özellikleri, onu diş hekimliğinde kullanım için öne çıkarmıştır. Ozonun endodontide antimikrobiyal olarak kullanım potansiyeli çok yüksektir. Endodontide ozonlanmış
su, ozonlanmış yağ ve ozon gazı formlarında irrigasyon solüsyonu ya da kanal içi medikament olarak kullanılabilir. Ozon endodontide postoperatif ağrı kontrolünde de kullanılabilir.
Kaynakça
- 1. Bocci V. Ozone as Janus: this controversial gas can be
either toxic or medically useful. Mediators Inflamm.
2004;13(1):3-11.
- 2. Saini R. Ozone therapy in dentistry: A strategic review. J
Nat Sci Biol Med. 2011;2(2):151-3.
- 3. Di Paolo N, Bocci V, Gaggioti E. Ozone therapy. Int J Artif
Organs. 2004;27(3):168-75.
- 4. Bocci VA. Scientific and medical aspects of ozone therapy.
State of the art. Arch Med Res. 2006;37(4):425-35.
- 5. Sen S, Sen S. Ozone therapy a new vista in dentistry:
integrated review. Medical Gas Research. 2020;10(4),189–
192.
- 6. Sujatha B, Kumar M, Pratap G, Vardhan R. Ozone therapy–A
paradigm shift in dentistry. Health Sci. 2013;2(3):1-10.
- 7. Azarpazhooh A, Limeback H. The application of ozone
in dentistry: a systematic review of literature. J Dent.
2008;36(2), 104–116.
- 8. McKenna DF, Borzabadi-Farahani A, Lynch E. The effect
of subgingival ozone and/or hydrogen peroxide on the
development of peri-implant mucositis: a double- blind
randomized controlled trial. Int J Oral Maxillofac Implants.
2013;28(6):1483-9.
- 9. Baumgartner JC, Cuenin PR. Efficacy of several
concentrations of sodium hypochlorite for root canal
irrigation. J Endod. 1992;18(12):605-12.
- 10. Brugnera A, Zanin F, Barbin EL, Spano JC, Santana
R, Pécora JD. Effects of Er: YAG and Nd: YAG laser
irradiation on radicular dentine permeability using different
irrigating solutions. Lasers Surg Med. 2003;33(4):256-9.
- 11. Song M, Zeng Q, Xiang Y, Gao L, Huang J, Huang J,
Wu K, Lu J. The antibacterial effect of topical ozone on
the treatment of MRSA skin infection. Mol Med Rep.
2018;17(2):2449–2455.
- 12. Tiwari S, Avinash A, Katiyar S, Aarthi Iyer A, Jain S. Dental
applications of ozone therapy: A review of literature. The
Saudi J Dent Res. 2017; 8(1-2):105- 111.
- 13. Silva EJNL, Prado MC, Soares DN, Hecksher F, Martins
JNR, Fidalgo TKS. The effect of ozone therapy in root
canal disinfection: a systematic review. Int Endod J.
2020;53(3):317-332.
- 14. Kaptan F, Güven EP, Topcuoglu N, Yazici M, Külekçi G. In
vitro assessment of the recurrent doses of topical gaseous
ozone in the removal of Enterococcus faecalis biofilms in
root canals. Niger J Clin Pract. 2014;17(5):573–578.
- 15. Polydorou O, Pelz K, Hahn P. Antibacterial effect of an
ozone device and its comparison with two dentin‐ bonding
systems. Eur J Oral Sci. 2006;114(4):349-53.
- 16. Sousa SIV, Alvim‐Ferraz MCM, Martins FG, Pereira MC.
Ozone exposure and its influence on the worsening of
childhood asthma. Allergy. 2009;64(7):1046-55.
- 17. Halbauer K, Prskalo K, Jankovic B, Tarle Z, Panduric V,
Kalenic S. Efficacy of ozone on microorganisms in the
tooth root canal. Coll Antropol. 2013;37(1):101-7.
- 18. Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA. An
in vitro evaluation of the ability of ozone to kill a strain of
Enterococcus faecalis. Int Endod J. 2005;38(1):22-9.
- 19. Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita
M, Nishihara T. Efficacy of ozone on survival and
permeability of oral microorganisms. Oral Microbiol
- 20. Thanomsub B, Anupunpisit V, Chanphetch S,
Watcharachaipong T, Poonkhum R, Srisukonth C. Effects of
ozone treatment on cell growth and ultrastructural changes
in bacteria. J Gen Appl Microbiol. 2002;48(4):193-9.
- 21. Baysan A, Whiley RA, Lynch E. Antimicrobial effect
of a novel ozone-Immunol. generating device on microorganisms
associated with primary root carious lesions in
vitro. Caries Res. 2000;34(6):498-501.
- 22. Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E,
Hollweck R, Hickel R, Brand K. Effect of ozone on oral
cells compared with established antimicrobials. Eur J Oral
Sci. 2006;114(5):435-40.
- 23. Bal FA, Ozkocak I, Cadirci BH, Karaarslan ES,
Cakdinleyen M, Agaccioglu M. Effects of photodynamic
therapy with indocyanine green on Streptococcus mutans
biofilm. Photodiagnosis Photodyn Ther. 2019;26:229-234.
- 24. Kapdan A, Öztaş N, Sümer Z. Comparing the antibacterial
activity of gaseous ozone and chlorhexidine solution on a
tooth cavity model. J Clin Exp Dent. 2013;5(3):e133-e137.
- 25. Pinheiro SL, da Silva CC, da Silva LA, Cicotti MP, da
Silveira Bueno CE, Fontana CE et al. Antimicrobial
efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine,
and ozonated water as irrigants in mesiobuccal root canals
with severe curvature of mandibular molars. Eur J Dent.
2018;12(1):94–99.
- 26. Agarwal S, Tyagi P, Deshpande A, Yadav S, Jain V, Rana
KS. Comparison of antimicrobial efficacy of aqueous ozone,
green tea, and normal saline as irrigants in pulpectomy
procedures of primary teeth. J Indian Soc Pedod Prev Dent.
2020;38(2):164-170.
- 27. Mehta N, Gupta A, Mahesh S, Abraham D, Singh A,
Jala S, Chauhan N, Sreen D. Comparative evaluation of
antibacterial efficacy of Allium sativum extract, aqueous
ozone, diode laser, and 3% sodium hypochlorite in root
canal disinfection: An in vivo study. J Conserv Dent. 2020;
23(6): 577–582.
- 28. Hubbezoglu I, Zan R, Tunc T, Sumer Z. Antibacterial
Efficacy of Aqueous Ozone in Root Canals Infected
by Enterococcus faecalis. Jundishapur J Microbiol.
2014;7(7):e11411.
- 29. Estrela C, Estrela CRA, Decurcio DA, Hollanda ACB,
Silva JA. Antimicrobial efficacy of ozonated water, gaseous
ozone, sodium hypochlorite and chlorhexidine in infected
human root canals. Int Endod J. 2007;40(2):85-93.
- 30. Moraes MM, Coelho MS, Nascimento WM, Nogales CG,
de Campos FUF, Soares AJ, Frozoni M. The antimicrobial
effect of different ozone protocols applied in severe curved
canals contaminated with Enterococcus faecalis: ex vivo
study. Odontology. 2021;109(3):696–700.
- 31. Boch T, Tennert C, Vach K, Al-Ahmad A, Hellwig E,
Polydorou O. Effect of gaseous ozone on Enterococcus
faecalis biofilm– an in vitro study. Clin Oral Investig.
2016;20(7):1733-39.
- 32. Küçük F, Yıldırım S, Çetiner S. Cytotoxicity assessment of
different doses of ozonated water on dental pulp cells. BMC
Oral Health. 2021;19;21(1):32.
- 33. Reddy KS, Prasad SD, Sirisha PD, Prashanth V. Evaluation
of Antimicrobial Efficacy of Ozonated Sesame Oil,
Calcium Hydroxide and their Combination as Intracanal
Medicament against Candida Albicans: An in-vitro study.
Adv Hum Biol. 2015;5(2):78-87.
- 34. Silveira AMV, Lopes HP, Siqueira Jr JF, Macedo SB,
Consolaro A. Periradicular repair after two-visit endodontic
treatment using two different intracanal medications
compared to single- visit endodontic treatment. Braz Dent
J. 2007;18(4):299-304.
- 35. Kriplani S, Sedani S, Mishra A, Umre U. Non-surgical
Management of Periapical Lesions With the Use of Newer
Modalities in Adjunct to the Conventional: A Case Series.
Cureus. 2024;16(3): e57314.
- 36. Vasavada K, Kapoor S. Evaluation of ozonized calcium
hydroxide as an effective intracanal medicament during
root canal procedures: an in vitro observational study. Med
Gas Res. 2020;10(3):122-124.
- 37. Khalilov R. Semptomatik Apikal Periodontitisli Molar
Dişlere Sahip Hastalarda Kanal İçi Ozon Uygulamasının
Postoperatif Ağrıya Etkisi: Randomize, Plasebo Kontrollü
Klinik Çalışma (Uzmanlık Tezi) Erzurum: Atatürk
Üniversitesi Diş Hekimliği Fakültesi, Endodonti Anabilim
Dalı;2017.
- 38. Sağlam H, Aladağ H. Comparison of ıntracanal ozone and
low‐level laser therapy on postoperative pain in vital teeth
with symptomatic apical periodontitis:placebo‐controlled
randomize trial. Lasers Med Sci. 2023; 38(1):227.
- 39. Sinha N, Asthana G, Parmar G, Langaliya A, Shah J,
Kumbhar A, Singh B. Evaluation of Ozone Therapy in
Endodontic Treatment of Teeth with Necrotic Pulp and
Apical Periodontitis: A Randomized Clinical Trial. J Endod.
2021;47(12):1820–1828.
- 40. Ahmed N, Suzuki S, Mosallam R. Effect of Oleozon on
Healing of Exposed Pulp Tissues. J Am Sci. 2011;7(5):38–
44.
- 41. Chandra SP, Chandrasekhar R, Uloopi KS, Vinay C, Kumar
NM. Success of root fillings with zinc oxide-ozonated oil
in primary molars: preliminary results. Eur Arch Paediatr
Dent. 2014;15(3):191-5.
- 42. El-Desouky SS, Omer SMM, Ghouraba RF, Ali Abdel Latif
RM, Kabbash IA, Hadwa SM. Zinc oxide-ozonated olive
oil as a new root canal filling material in primary molars:
a clinical randomized controlled trial. Clin Oral Investig.
2023;27(12):7395-7405.
- 43. Maiya A. Applications of ozone in dentistry. Int J Clin Den
Sci. 2011;2:23-7.
- 44. Menzel DB. Ozone: an overview of its toxicity in man and
animals. J Toxicol Environ Health. 1984;13(2- 3):181-204.
- 45. Pattanaik B, Jetwa D, Pattanaik S, Manglekar S, Naitam
DN, Dani A. Ozone therapy in dentistry: a literature review.
J Interdisciplinary Dent. 2011;1(2):87.