Verbascum sinuatum'un Çiçek Kısımlarının Antioksidan Potansiyeli, Verbascoside ve Şeker Bileşiminin Değerlendirilmesi

Öz

Bu çalışmada, Verbascum sinuatum bitkisinin çiçek kısımlarından elde edilen hidrometanolik ekstraktın antioksidan kapasitesi değerlendirilmiş ve toplam fenolik, flavonoid, verbaskosit ve şeker içerikleri belirlenmiştir. Ekstraktın antioksidan kapasitesi DPPH, ABTS ve FRAP analizleri kullanılarak değerlendirilmiştir. Toplam fenolik ve flavonoid içerikleri spektrofotometrik yöntemlerle ölçülürken, ekstraktın verbaskosit ve şeker içerikleri sırasıyla HPLC-UV ve HPLC-RID yöntemleri ile kantitatif olarak analiz edilmiştir. Elde edilen sonuçlara göre, ekstraktın DPPH serbest radikal giderme aktivitesi IC₅₀ = 30.70±1.76 µg/mL, ABTS değeri IC₅₀ = 17.31±0.65 µg/mL olarak bulunmuştur. FRAP aktivitesi ise 63.94±0.98 mg TE/g ekstrakt olarak belirlenmiştir. Toplam fenolik içerik 45.23±0.77 mg GAE/g, toplam flavonoid içerik ise 6.31±0.50 mg QE/g olarak ölçülmüştür. HPLC-UV analizine göre, ekstraktın verbaskosit içeriği 9.72±0.85 mg/g olarak tespit edilmiştir. Ayrıca, sonuçlar çiçek ekstraktında en yüksek miktarda bulunan şekerin fruktoz (190.39 mg/g) olduğunu, bunu glukozun (132.91 mg/g) izlediğini ve nispeten düşük miktarda sakkarozun (7.21 mg/g) bulunduğunu göstermiştir. Sonuç olarak, V. sinuatum çiçeklerinin hidrometanolik ekstraktı belirgin düzeyde antioksidan aktivite sergilemiş, özellikle verbaskosit başta olmak üzere önemli miktarda fenolik bileşik içermiş ve fruktoz ile glukoz gibi heksoz şekerleri bakımından zengin bulunmuştur. Bu bulgular, söz konusu bitkinin doğal antioksidan ve biyoaktif bileşik kaynağı olarak değerlendirilme potansiyeline sahip olduğunu göstermektedir.

Anahtar Kelimeler

• Verbascum sinuatum
• antioksidan aktivite
• şeker bileşimi
• HPLC analizi.
• Verbaskosit

Assessment of antioxidant potential, verbascoside, and sugar composition of the floral parts of verbascum sinuatum

Öz

In this study, the antioxidant capacity of the hydromethanolic extract obtained from the floral parts of Verbascum sinuatum was evaluated, and its total phenolic, flavonoid, verbascoside, and sugar contents were determined. The antioxidant capacity of the extract was evaluated using DPPH, ABTS, and FRAP assays. Total phenolic and flavonoid contents were measured spectrophotometrically, while the verbascoside and sugar contents of the extract were quantified by the HPLC-UV and HPLC-RID methods, respectively. According to the results, the DPPH radical scavenging activity of the extract was found to be IC50= 30.70±1.76 µg/mL, and the ABTS value was IC50= 17.31±0.65 µg/mL. The FRAP activity was determined as 63.94±0.98 mg TE/g extract. The total phenolic content was measured as 45.23 0.77 mg GAE/g, and the total flavonoid content as 6.31±0.50 mg QE/g. According to the HPLC-UV analysis, the verbascoside content of the extract was found to be 9.72±0.85 mg/g. Additionally, the results demonstrated that fructose (190.39 mg/g) was the most abundant sugar in the floral extract of V. sinuatum, followed by glucose (132.91 mg/g) and a relatively small amount of sucrose (7.21 mg/g). As a result, the hydromethanolic extract of V. sinuatum floral parts exhibited a considerable level of antioxidant activity, contained significant amounts of phenolic compounds, particularly verbascoside, and was rich in hexose sugars, especially fructose and glucose. These findings suggest that this plant may be considered a promising natural source of antioxidants and bioactive compounds.

Anahtar Kelimeler

• Verbascum sinuatum
• verbascoside
• antioxidant activity
• sugar composition
• HPLC analysis.

Kaynakça

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