Removal of coomassie brilliant blue G-250 from aqueous solutions by Purolite A420S anion-exchange resin
Öz
In this study, the removal of the toxic dye Coomassie Brilliant Blue G-250 (CBB) from aqueous solutions was investigated using Purolite A420S, a strong base anion-exchange resin. Batch experiments were conducted to evaluate the effects of resin dosage, solution pH, contact time, initial dye concentration, and temperature on the ion-exchange performance. The optimum resin dosage was determined as 0.05 g per 25 mL solution, yielding a maximum removal efficiency of approximately 91%. The removal efficiency was found to be relatively insensitive to pH in the range of 5–9. Kinetic studies indicated rapid initial uptake followed by equilibrium after 240 min, and the experimental data were best described by the pseudo-second-order kinetic model. Equilibrium isotherm analysis showed that CBB sorption followed the Langmuir isotherm model, with a maximum sorption capacity of 5.17 mg/g. Thermodynamic parameters revealed that the sorption process is exothermic and accompanied by a decrease in entropy, indicating increased order at the solid–solution interface during ion exchange.
Anahtar Kelimeler
Coomassie Brilliant Blue G-250 ion exchange Purolite A420S anion-exchange resin dye removal
Etik Beyan
-
Destekleyen Kurum
The Scientific and Technological Research Council of Türkiye
Proje Numarası
2209-A
Teşekkür
The authors would like to thank Purolite International Co. (Turkey) for kindly providing the ion-exchange resin samples used in this study.
Kaynakça
- Ü. Ecer, Ş. Yılmaz, T. Şahan, Synthesis, Characterization, and application of Ag-Doped Mercapto-Functionalized clay for decolorization of Coomassie brilliant Blue: Optimization using RSM, Chem Phys Lett, 825, 2023, 140610
- S. Ata, M.I. Din, A. Rasool, I. Qasim, I.U. Mohsin, Equilibrium, Thermodynamics, and Kinetic Sorption Studies for the Removal of Coomassie Brilliant Blue on Wheat Bran as a Low-Cost Adsorbent, J Anal Methods Chem, 2012, 2012, 405980
- N. Mekaoui, J. Chamieh, V. Dugas, C. Demesmay, A. Berthod, Purification of Coomassie Brilliant Blue G-250 by multiple dual mode countercurrent chromatography, J Chromatogr A, 1232, 2012, 134–141
- N. Kerabchi, M.L. Djaballah, Z. Boutamine, A. Latreche, A. Benalia, D. Kerroum, A. Pizzi, A. Panico, Impact of pH and PS Concentration on the Thermal Degradation of Brilliant Coomassie Blue G-250: An Experimental and Modeling Approach, Water, 17, 2025, 3008
- H.K. Ibrahim, M.A. Al-Da’ Amy, E.T. Kreem, Decolorization of coomassie brilliant blue G-250 dye using snail shell powder by action of adsorption processes, Res J Pharm Technol, 12, 2019, 4921–4925
- P. Moradihamedani, Recent advances in dye removal from wastewater by membrane technology: a review, Polym Bull, 79, 2022, 2603–2631
- R.S. Dassanayake, S. Acharya, N. Abidi, Recent Advances in Biopolymer-Based Dye Removal Technologies, Molecules, 26, 2021, 4697
- H.M. Solayman, M.A. Hossen, A.A. Aziz, N.Y. Yahya, K.H. Leong, L.C. Sim, M.U. Monir, K.D. Zoh, Performance evaluation of dye wastewater treatment technologies: A review, J Environ Chem Eng, 11, 2023, 109610
- E. Forgacs, T. Cserháti, G. Oros, Removal of synthetic dyes from wastewaters: a review, Environ Int, 30, 2004, 953–971
- P. Vijayan P, P.G. Chithra, A.K.S. V, E.B. Ansar, J. Parameswaranpillai, Development and Current Trends on Ion Exchange Materials, Sep Purif Rev, 53, 2024, 40–60
- M. Berrios, J.A. Siles, M.A. Martín, A. Martín, Ion Exchange, Sep Purif Technol Biorefineries, Editors: S. Ramaswamy, H.J. Huang, B.V. Ramarao, 2013, West Sussex, Wiley
- C.A. Pohl, J.R. Stillian, P.E. Jackson, Factors controlling ion-exchange selectivity in suppressed ion chromatography, J Chromatogr A, 789, 1997, 29–41
- I. Levchuk, J.J.R. Márquez, M. Sillanpää, Removal of natural organic matter (NOM) from water by ion exchange – A review, Chemosphere, 192, 2018, 90–104
- K. Grintzalis, C.D. Georgiou, Y.J. Schneider, An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination, Anal Biochem, 480, 2015, 28–30
- M. İmamoğlu, S. Uzun, Removal of Cu(II) ions from aqueous solutions using sunflower stalk biochar prepared with sulfuric acid, Turkish J Anal Chem, 7, 2025, 292–299
- P. Bozbeyoglu, A. Gündoğdu, Adsorption of hexavalent chromium from aqueous solution onto corn cobs – activated carbon, Turkish J Anal Chem, 5, 2023, 107–117
- B. Kabak, Magnetic biochar derived from olive pomace for efficient tetracycline removal from water, Turkish J Anal Chem, 7, 2025, 268–281
- H.J. Chial, H.B. Thompson, A.G. Splittgerber, A Spectral Study of the Charge Forms of Coomassie Blue G, Anal Biochem, 209, 1993, 258–266
- Ö. Arar, Shallow Shell resin versus traditional resin: A case study for Cu(II) removal, Anadolu Univ J Sci Technol Appl Sci Eng, 17, 2016, 530–542
- Y.S. Ho, Second-order kinetic model for the sorption of cadmium onto tree fern: A comparison of linear and non-linear methods, Water Res, 40, 2006, 119–125
- Y.S. Ho, G. McKay, Comparative sorption kinetic studies of dye and aromatic compounds onto fly ash, J Environ Sci Heal Part A, 34, 1999, 1179–1204
- Y.S. Ho, G. Mckay, Kinetic Models for the Sorption of Dye from Aqueous Solution by Wood, Process Saf Environ Prot, 76, 1998, 183–191
- A.E. Yayayürük, O. Yayayürük, Adsorptive performance of nanosized zero-valent iron for V(V) removal from aqueous solutions, J Chem Technol Biotechnol, 92, 2017, 1891–1898
- B. Alyüz, S. Veli, Kinetics and equilibrium studies for the removal of nickel and zinc from aqueous solutions by ion exchange resins, J Hazard Mater, 167, 2009, 482–488
- M. Abbas, A. Cherfi, S. Kaddour, T. Aksil, Adsorption in simple batch experiments of Coomassie blue G-250 by apricot stone activated carbon—Kinetics and isotherms modelling, Desalin Water Treat, 57, 2016, 15037–15048
- H. Wang, H. Xing, K. Yan, D. Han, J. Chen, Oyster shell derived hydroxyapatite microspheres as an effective adsorbent for remediation of Coomassie brilliant blue, Adv Powder Technol, 33, 2022, 103425
- P. Ilgin, A. Gur, Synthesis and characterization of a new fast swelling poly(EPMA-co-METAC) as superabsorbent polymer for anionic dye absorbent, Iran Polym J, 24, 2015, 149–159
- R. Mallampati, K.S. Tan, S. Valiyaveettil, Utilization of corn fibers and luffa peels for extraction of pollutants from water, Int Biodeterior Biodegradation, 103, 2015, 8–15
- P.F. de Sales, Z.M. Magriotis, M.A.L.S. Rossi, R.F. Resende, C.A. Nunes, Optimization by Response Surface Methodology of the adsorption of Coomassie Blue dye on natural and acid-treated clays, J Environ Manage, 130, 2013, 417–428
- P. Sricharoen, S. Kongsri, C. Kukusamude, Y. Areerob, P. Nuengmatcha, S. Chanthai, N. Limchoowong, Ultrasound-irradiated synthesis of 3-mercaptopropyl trimethoxysilane-modified hydroxyapatite derived from fish-scale residues followed by ultrasound-assisted organic dyes removal, Sci Rep, 11, 2021, 5560
- S. Dhananasekaran, R. Palanivel, S. Pappu, Adsorption of Methylene Blue, Bromophenol Blue, and Coomassie Brilliant Blue by α-chitin nanoparticles, J Adv Res, 7, 2016, 113–124
- A. Raj, A. Yadav, A.P. Rawat, A.K. Singh, S. Kumar, A.K. Pandey, R. Sirohi, A. Pandey, Kinetic and thermodynamic investigations of sewage sludge biochar in removal of Remazol Brilliant Blue R dye from aqueous solution and evaluation of residual dyes cytotoxicity, Environ Technol Innov, 23, 2021, 101556
- M. El-Kammah, E. Elkhatib, S. Gouveia, C. Cameselle, E. Aboukila, Enhanced removal of Indigo Carmine dye from textile effluent using green cost-efficient nanomaterial: Adsorption, kinetics, thermodynamics and mechanisms, Sustain Chem Pharm, 29, 2022, 100753
- S. Zghal, I. Jedidi, M. Cretin, S. Cerneaux, M. Abdelmouleh, Adsorptive Removal of Rhodamine B Dye Using Carbon Graphite/CNT Composites as Adsorbents: Kinetics, Isotherms and Thermodynamic Study, Materials (Basel), 16, 2023, 1015
- J.D. Morgan, D.H. Napper, G.G. Warr, Thermodynamics of Ion Exchange Selectivity at Interfaces, 1995
- T. Shahwan, Critical insights into the limitations and interpretations of the determination of ∆Go, ∆Ho, and ∆So of sorption of aqueous pollutants on different sorbents, Colloid Interface Sci Commun, 41, 2021, 100369
- R.M. Barrer, L.V.C. Rees, D.J. Ward, Thermochemistry and thermodynamics of ion exchange in a crystalline exchange medium, Proc R Soc London Ser A Math Phys Sci, 273, 1963, 180–197
- J. Novosad, A.L. Myers, Thermodynamics of ion exchange as an adsorption process, Can J Chem Eng, 60, 1982, 500–503.
Öz
Bu çalışmada; kuvvetli bazik anyon değiştirici olan Purolite A420S kullanılarak sulardan Coomassie Brilliant Blue G-250 (CBB) giderimi incelenmiştir. Sulardan CBB giderimine; optimum reçine miktarı , çözelti pH`ı, temas süresi, derişim ve sıcaklığın etkisi incelenmiştir. Yapılan deneysel çalışmalar sonucunda optimum reçine miktarı 0,05 g/25 mL çözelti olarak bulunmuştur. CBB giderimi için optimum çalışma pH aralığı, 5–9 olarak belirlenmiştir. Gerçekleştirilen kinetik çalışmalarda, Purolite A420S reçinesi yaklaşık 240 dakika sonunda CBB’nin %91’ini gidermiştir. Yapılan denge izoterm çalışmaları, CBB gideriminin Langmuir izoterm modeline uygunluk gösterdiğini ortaya koymuştur. Termodinamik çalışmalar, sorpsiyon sürecinin ekzotermik olduğunu ve iyon değişimi sırasında katı–çözelti ara yüzeyinde düzensizliğin azaldığını göstermiştir.
Anahtar Kelimeler
Coomassie Brilliant Blue G-250 iyon değişimi anyon değiştirici reçine Purolite A420S boya giderimi
Etik Beyan
-
Destekleyen Kurum
Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
Proje Numarası
2209-A
Teşekkür
Bu çalışmada kullanılan iyon değiştirici reçine örneklerini sağladıkları için Purolite International Co. (Türkiye)’ye teşekkür ederiz
Kaynakça
- Ü. Ecer, Ş. Yılmaz, T. Şahan, Synthesis, Characterization, and application of Ag-Doped Mercapto-Functionalized clay for decolorization of Coomassie brilliant Blue: Optimization using RSM, Chem Phys Lett, 825, 2023, 140610
- S. Ata, M.I. Din, A. Rasool, I. Qasim, I.U. Mohsin, Equilibrium, Thermodynamics, and Kinetic Sorption Studies for the Removal of Coomassie Brilliant Blue on Wheat Bran as a Low-Cost Adsorbent, J Anal Methods Chem, 2012, 2012, 405980
- N. Mekaoui, J. Chamieh, V. Dugas, C. Demesmay, A. Berthod, Purification of Coomassie Brilliant Blue G-250 by multiple dual mode countercurrent chromatography, J Chromatogr A, 1232, 2012, 134–141
- N. Kerabchi, M.L. Djaballah, Z. Boutamine, A. Latreche, A. Benalia, D. Kerroum, A. Pizzi, A. Panico, Impact of pH and PS Concentration on the Thermal Degradation of Brilliant Coomassie Blue G-250: An Experimental and Modeling Approach, Water, 17, 2025, 3008
- H.K. Ibrahim, M.A. Al-Da’ Amy, E.T. Kreem, Decolorization of coomassie brilliant blue G-250 dye using snail shell powder by action of adsorption processes, Res J Pharm Technol, 12, 2019, 4921–4925
- P. Moradihamedani, Recent advances in dye removal from wastewater by membrane technology: a review, Polym Bull, 79, 2022, 2603–2631
- R.S. Dassanayake, S. Acharya, N. Abidi, Recent Advances in Biopolymer-Based Dye Removal Technologies, Molecules, 26, 2021, 4697
- H.M. Solayman, M.A. Hossen, A.A. Aziz, N.Y. Yahya, K.H. Leong, L.C. Sim, M.U. Monir, K.D. Zoh, Performance evaluation of dye wastewater treatment technologies: A review, J Environ Chem Eng, 11, 2023, 109610
- E. Forgacs, T. Cserháti, G. Oros, Removal of synthetic dyes from wastewaters: a review, Environ Int, 30, 2004, 953–971
- P. Vijayan P, P.G. Chithra, A.K.S. V, E.B. Ansar, J. Parameswaranpillai, Development and Current Trends on Ion Exchange Materials, Sep Purif Rev, 53, 2024, 40–60
- M. Berrios, J.A. Siles, M.A. Martín, A. Martín, Ion Exchange, Sep Purif Technol Biorefineries, Editors: S. Ramaswamy, H.J. Huang, B.V. Ramarao, 2013, West Sussex, Wiley
- C.A. Pohl, J.R. Stillian, P.E. Jackson, Factors controlling ion-exchange selectivity in suppressed ion chromatography, J Chromatogr A, 789, 1997, 29–41
- I. Levchuk, J.J.R. Márquez, M. Sillanpää, Removal of natural organic matter (NOM) from water by ion exchange – A review, Chemosphere, 192, 2018, 90–104
- K. Grintzalis, C.D. Georgiou, Y.J. Schneider, An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination, Anal Biochem, 480, 2015, 28–30
- M. İmamoğlu, S. Uzun, Removal of Cu(II) ions from aqueous solutions using sunflower stalk biochar prepared with sulfuric acid, Turkish J Anal Chem, 7, 2025, 292–299
- P. Bozbeyoglu, A. Gündoğdu, Adsorption of hexavalent chromium from aqueous solution onto corn cobs – activated carbon, Turkish J Anal Chem, 5, 2023, 107–117
- B. Kabak, Magnetic biochar derived from olive pomace for efficient tetracycline removal from water, Turkish J Anal Chem, 7, 2025, 268–281
- H.J. Chial, H.B. Thompson, A.G. Splittgerber, A Spectral Study of the Charge Forms of Coomassie Blue G, Anal Biochem, 209, 1993, 258–266
- Ö. Arar, Shallow Shell resin versus traditional resin: A case study for Cu(II) removal, Anadolu Univ J Sci Technol Appl Sci Eng, 17, 2016, 530–542
- Y.S. Ho, Second-order kinetic model for the sorption of cadmium onto tree fern: A comparison of linear and non-linear methods, Water Res, 40, 2006, 119–125
- Y.S. Ho, G. McKay, Comparative sorption kinetic studies of dye and aromatic compounds onto fly ash, J Environ Sci Heal Part A, 34, 1999, 1179–1204
- Y.S. Ho, G. Mckay, Kinetic Models for the Sorption of Dye from Aqueous Solution by Wood, Process Saf Environ Prot, 76, 1998, 183–191
- A.E. Yayayürük, O. Yayayürük, Adsorptive performance of nanosized zero-valent iron for V(V) removal from aqueous solutions, J Chem Technol Biotechnol, 92, 2017, 1891–1898
- B. Alyüz, S. Veli, Kinetics and equilibrium studies for the removal of nickel and zinc from aqueous solutions by ion exchange resins, J Hazard Mater, 167, 2009, 482–488
- M. Abbas, A. Cherfi, S. Kaddour, T. Aksil, Adsorption in simple batch experiments of Coomassie blue G-250 by apricot stone activated carbon—Kinetics and isotherms modelling, Desalin Water Treat, 57, 2016, 15037–15048
- H. Wang, H. Xing, K. Yan, D. Han, J. Chen, Oyster shell derived hydroxyapatite microspheres as an effective adsorbent for remediation of Coomassie brilliant blue, Adv Powder Technol, 33, 2022, 103425
- P. Ilgin, A. Gur, Synthesis and characterization of a new fast swelling poly(EPMA-co-METAC) as superabsorbent polymer for anionic dye absorbent, Iran Polym J, 24, 2015, 149–159
- R. Mallampati, K.S. Tan, S. Valiyaveettil, Utilization of corn fibers and luffa peels for extraction of pollutants from water, Int Biodeterior Biodegradation, 103, 2015, 8–15
- P.F. de Sales, Z.M. Magriotis, M.A.L.S. Rossi, R.F. Resende, C.A. Nunes, Optimization by Response Surface Methodology of the adsorption of Coomassie Blue dye on natural and acid-treated clays, J Environ Manage, 130, 2013, 417–428
- P. Sricharoen, S. Kongsri, C. Kukusamude, Y. Areerob, P. Nuengmatcha, S. Chanthai, N. Limchoowong, Ultrasound-irradiated synthesis of 3-mercaptopropyl trimethoxysilane-modified hydroxyapatite derived from fish-scale residues followed by ultrasound-assisted organic dyes removal, Sci Rep, 11, 2021, 5560
- S. Dhananasekaran, R. Palanivel, S. Pappu, Adsorption of Methylene Blue, Bromophenol Blue, and Coomassie Brilliant Blue by α-chitin nanoparticles, J Adv Res, 7, 2016, 113–124
- A. Raj, A. Yadav, A.P. Rawat, A.K. Singh, S. Kumar, A.K. Pandey, R. Sirohi, A. Pandey, Kinetic and thermodynamic investigations of sewage sludge biochar in removal of Remazol Brilliant Blue R dye from aqueous solution and evaluation of residual dyes cytotoxicity, Environ Technol Innov, 23, 2021, 101556
- M. El-Kammah, E. Elkhatib, S. Gouveia, C. Cameselle, E. Aboukila, Enhanced removal of Indigo Carmine dye from textile effluent using green cost-efficient nanomaterial: Adsorption, kinetics, thermodynamics and mechanisms, Sustain Chem Pharm, 29, 2022, 100753
- S. Zghal, I. Jedidi, M. Cretin, S. Cerneaux, M. Abdelmouleh, Adsorptive Removal of Rhodamine B Dye Using Carbon Graphite/CNT Composites as Adsorbents: Kinetics, Isotherms and Thermodynamic Study, Materials (Basel), 16, 2023, 1015
- J.D. Morgan, D.H. Napper, G.G. Warr, Thermodynamics of Ion Exchange Selectivity at Interfaces, 1995
- T. Shahwan, Critical insights into the limitations and interpretations of the determination of ∆Go, ∆Ho, and ∆So of sorption of aqueous pollutants on different sorbents, Colloid Interface Sci Commun, 41, 2021, 100369
- R.M. Barrer, L.V.C. Rees, D.J. Ward, Thermochemistry and thermodynamics of ion exchange in a crystalline exchange medium, Proc R Soc London Ser A Math Phys Sci, 273, 1963, 180–197
- J. Novosad, A.L. Myers, Thermodynamics of ion exchange as an adsorption process, Can J Chem Eng, 60, 1982, 500–503.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Separasyon Bilimi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | 2209-A |
| Gönderilme Tarihi | 21 Aralık 2025 |
| Kabul Tarihi | 26 Ocak 2026 |
| Yayımlanma Tarihi | 31 Ocak 2026 |
| Yayımlandığı Sayı | Yıl 2026 Cilt: 8 Sayı: 1 |