Psephellus huber-morathii Wagenitz’in Uçucu Bileşiminin HD, SPME ve n-Heksan Ekstraksiyonu Kullanılarak Karşılaştırmalı Değerlendirilmesi ve Antioksidan Aktivitesi

Öz

Bu çalışmanın amacı, Psephellus huber-morathii Wagenitz’in toprak üstü kısımlarının uçucu bileşimi ve antioksidan aktivitesini; hidrodistilasyon (HD), katı faz mikroekstraksiyon (SPME) ve n-heksan ekstraksiyonu yöntemleriyle karşılaştırmalı olarak değerlendirmektir. Kimyasal profiller GC-MS ile analiz edilmiş ve sırasıyla 26, 13 ve 33 olmak üzere toplam 60 bileşik tanımlanmış; bu bileşikler toplam kompozisyonun %98,2, %99,2 ve %99,3’ünü oluşturmuştur. Başlıca bileşenler; uçucu yağda (EO) n-heksadekanoik asit (%22,1), spathulenol (%12,1), karyofilen oksit (%8,3) ve α-pinen (%7,3); SPME ekstraktında hekzanal (%20,6), 1,3,5-trimetilbenzen (%18,0) ve 1-etil-4-metilbenzen (%12,5); n-heksan ekstraktının SPME’sinde ise 1,3,5-trimetilbenzen (%37,1) ve 1-etil-4-metilbenzen (%26,6) olarak belirlenmiştir. Antioksidan aktiviteler, uçucu yağ (EO), n-heksan ve asetonitril ekstraktları için FRAP, CUPRAC ve DPPH yöntemleri kullanılarak değerlendirilmiştir. Asetonitril ekstraktı, FRAP (1433 ± 4,39 µM TE/g) ve CUPRAC (1013 ± 4,02 µM TE/g) testlerinde en yüksek etkiyi gösterirken, uçucu yağ DPPH serbest radikal süpürücü aktivitede en güçlü etkiyi sergilemiştir (IC₅₀: 0,0612 ± 0,0020 mg/mL).

Anahtar Kelimeler

• Psephellus huber-morathii
• SPME
• uçucu yağ
• antioksidan

Comparative evaluation of volatile composition of Psephellus huber-morathii Wagenitz using HD, SPME, and n-hexane extract and antioxidant activity

Öz

The present study aimed to comparatively evaluate the volatile composition and antioxidant activity of the aerial parts of Psephellus huber-morathii Wagenitz obtained by hydrodistillation (HD), solid-phase microextraction (SPME), and n-hexane extraction. The chemical profiles were analyzed by GC-MS, resulting in the identification of 60 compounds (26, 13, and 33, each), accounting for 98.2% 99.2%, and 99.3% of the total composition, respectively. The major constituents were identified as n-hexadecanoic acid (22.1%), spathulenol (12.1%), caryophyllene oxide (8.3%), and α-pinene (7.3%) in the essential oil (EO); hexanal (20.6%), 1,3,5-trimethylbenzene (18.0%), and 1-ethyl-4-methylbenzene (12.5%) in the SPME extract; and 1,3,5-trimethylbenzene (37.1%) and 1-ethyl-4-methylbenzene (26.6%) in the SPME of n-hexane extract. Antioxidant activities were assessed for EO, n-hexane, and acetonitrile extracts using the FRAP, CUPRAC, and DPPH assays. The acetonitrile extract was the most effective against FRAP (1433 ± 4.39 µM TE/g) and CUPRAC (1013 ± 4.02 µM TE/g) values, whereas the EO showed the strongest DPPH radical scavenging activity (IC₅₀: 0.0612 ± 0.0020 mg/mL).

Anahtar Kelimeler

• Psephellus huber-morathii
• SPME
• essential oil
• antioxidant

Kaynakça

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