Isabella Üzüm Posası Ekstresinin Antilipidemik, Antidiyabetik ve Antioksidan Aktiviteleri: Deneysel Tasarım Yaklaşımı

Öz

Gıda işleme yan ürünleri, gıda, ilaç, kozmetik ve doğal renklendirici endüstrilerinde uygulamaları olan biyoaktif bileşiklerin değerli kaynakları olarak kabul edilmektedir. Bu çalışma, Isabella üzümü (Vitis labrusca) posasının fitokimyasal bileşimini ve pankreatik lipaz, kolesterol esteraz ve α-glukozidaz inhibitör aktivitelerini araştırmayı amaçlamıştır. Ekstraktlar, deney tasarımı yaklaşımı kullanılarak Isabella üzümü posasından hazırlanmıştır. Ekstraktların fenolik içeriği RP-HPLC ile analiz edilmiş ve ekstraksiyon parametrelerinin etkileri değerlendirilmiştir. Etanol konsantrasyonu, mikrodalga gücü ve ekstraksiyon sıcaklığı en etkili faktörler olarak belirlenmiştir. Ekstraksiyon koşullarını optimize etmek için Box-Behnken tasarımı kullanılmıştır. Optimize edilmiş ekstrakt, antioksidan ve enzim inhibitör aktiviteleri açısından daha ayrıntılı olarak değerlendirilmiştir. Sonuçlar, optimize edilmiş ekstraktın pankreatik lipaz, kolesterol esteraz ve α-glukozidaz enzimlerine karşı inhibitör etkiler gösterdiğini ortaya koymuştur. Ek olarak, CUPRAC, FRAP ve DPPH radikal temizleme testleri kullanılarak güçlü antioksidan aktivite doğrulandı. Bu bulgular, Isabella üzüm posasının antioksidan ve metabolik enzim inhibitör potansiyeline sahip biyoaktif bileşiklerin umut vadeden doğal bir kaynağı olarak değerlendirilebileceğini göstermektedir. Bu nedenle, üzüm işleme yan ürünleri, oksidatif stresi ve obezite ve diyabet gibi metabolik bozuklukları hedefleyen fonksiyonel bileşenlerin ve nutrasötik adaylarının geliştirilmesine katkıda bulunabilir.

Anahtar Kelimeler

• Vitis labrusca
• Box-Behnken
• Lipaz
• Kolesterol esteraz

Proje Numarası

TYL-2023-10977

Antilipidemic, Antidiabetic and Antioxidant Activities of Isabella Grape Pomace Extract: A Design of Experiments Approach

Öz

The food processing by-products are considered valuable sources of bioactive compounds with applications in the food, pharmaceutical, cosmetic, and natural colorant industries. are considered valuable sources of bioactive compounds with applications in the food, pharmaceutical, cosmetic, and natural colorant industries. The present study aimed to investigate the phytochemical composition and the pancreatic lipase, cholesterol esterase, and α-glucosidase inhibitory activities of Isabella grape (Vitis labrusca) pomace. Extracts were prepared from Isabella grape pomace using a design of experiments approach. The phenolic content of the extracts was analyzed by RP-HPLC, and the effects of extraction parameters were evaluated. Ethanol concentration, microwave power, and extraction temperature were identified as the most influential factors. A Box-Behnken design was employed to optimize the extraction conditions. The optimized extract was further evaluated for its antioxidant and enzyme inhibitory activities. The results demonstrated that the optimized extract exhibited inhibitory effects against pancreatic lipase, cholesterol esterase, and α-glucosidase enzymes. In addition, strong antioxidant activity was confirmed using CUPRAC, FRAP, and DPPH DPPH radical scavenging assays. These findings indicate that Isabella grape pomace could be considered a promising natural source of bioactive compounds with antioxidant and metabolic enzyme inhibitory potential. Therefore, grape processing by-products may contribute to the development of functional ingredients and nutraceutical candidates targeting oxidative stress and metabolic disorders such as obesity and diabetes.

Anahtar Kelimeler

• Vitis labrusca
• Lipase
• Cholesterol esterase
• α-Glucosidase
• Box-Behnken

Destekleyen Kurum

Office of Scientific Research Projects of Karadeniz Technical University

Proje Numarası

TYL-2023-10977

Etik Beyan

The authors declare no conflict of interest.

Teşekkür

This work was supported by Office of Scientific Research Projects of Karadeniz Technical University (Project number: TYL-2023-10977).

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