Evaluation of biological activities of silver nanoparticles (AgNPs) synthesized by green nanotechnology from birch (Betula spp.) branches extract

Yıl 2023, Cilt: 5 Sayı: 2, 151 - 161, 29.12.2023

Burçin Özçelik Aslı Kara

https://doi.org/10.51435/turkjac.1401931

Öz

Green synthesis of silver nanoparticles (AgNPs) provide superiority due to their usage of various biological applications. The aim of this study is synthesizing the silver nanoparticles by using Birch (Betula spp.) branches extract with an ecofriendly, cost-effective, simple, and cheap green method. Even Betula is wide-spread tree with rich in phenolic compounds, data on the use of Betula branches is limited. Within this scope, this study is the first for using Betula branche extracts which take part as reducing and capping agent to synthesize the silver nanoparticles to evaluate the antimicrobial activity and antiproliferative efficiency. The biosynthesized AgNPs were characterized by various characterization methods such as UV−visible spectroscopy, dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The characterization analysis has revealed the phenolic compounds of Betula extract acted as reducing and capping agent for formation of AgNPs.
The synthesized selected AgNPs were exhibited spherical shape with 103.2 ± 5.2 and 69.2 ±12.7 nm according to DLS and SEM analysis, respectively. Also, the biological activity of biosynthesized AgNPs were evaluated by antimicrobial and antiproliferative tests on selected microorganisms and cell line, respectively. The IC50 values of B3-4 AgNPs was determined as 64.27 µg/mL on HT29 colorectal cancer cells. And also, the antimicrobial activity results of AgNPs have revealed the dose-dependent inhibition for all studied test microorganisms.
In conclusion, this study evidently suggests the use of silver nanoparticles biosynthesized from Betula branches extract as a potential agent for antimicrobial and anticancer studies.

Anahtar Kelimeler

Betula spp., silver nanoparticles, green synthesis, antiproliferative, colorectal cancer, antimicrobial.

Kaynakça

• S. Bayda,1, M. Adeel, T. Tuccinardi, M. Cordani, F. Rizzolio, The History of Nanoscience and Nanotechnology: From Chemical–Physical Applications to Nanomedicine, Molecules, 2020, PMC6982820.
• M. K. Sahu, R. Yadav, S. P. Tiwari, Recent advances in nanotechnology, International Journal of Nanomaterials, Nanotechnology and Nanomedicine, 2023, 3492.000053. H. Özcan, A. Dalmaz, M. Özdinçer, K. Zenkin, S. Durmuş, Biosynthesis and characterization of α-FeOOH nanoparticles using Isabella grape (Vitis Labrusca L.) extract, Turk J Anal Chem, 2023, 2687–6698.
• S. Nimesh, Nanotechnology: an introduction, Gene Therapy, 2013, USA, Elsevier.
• A. Afkhami, T. Madrakian, M. Ahmadi, Chapter - Nanotechnology and analytical chemistry, Analytical Nanochemistry, 2023, İndia, Elsevier.
• A. Surendiran, S. Sandhiya, S.C. Pradhan, C. Adithan, Novel applications of nanotechnology in medicine, Indian J Med Res, 2009, A229530832.
• U. Yezdani, M. G. Khan, N. Kushwah, A. Verma, F. Khan, Application of Nanotechnology in Diagnosis and Treatment of Various Diseases and Its Future, World J Pharm Pharm Sci, 2018, 2278 – 4357. S. Zaib, J. Iqbal, Nanotechnology: Applications, techniques, approaches, and the advancement in toxicology and environmental impact of engineered nanomaterials, Importance and Applications of Nanotechnology, 2019, MedDocs Publishers LLC, USA.
• W. Abdussalam-Mohammed, Review of Therapeutic Applications of Nanotechnology in Medicine Field and its Side Effects, J Chem Reviews, 2019.
• S. Malik, K. Muhammad, Y. Waheed, Emerging Applications of Nanotechnology in Healthcare and Medicine, 2023, PMC10536529.
• E. Abbasi, M. Milani, S. Fekri Aval, M .Kouhi, A .Akbarzadeh, H. Tayefi Nasrabadi, P. Nikasa, S.W. Joo, Y. Hanifehpour, K. Nejati-Koshki, M. Samiei, Silver nanoparticles: Synthesis methods, bio-applications and properties. Crit Rev Microbiol, 24937409.
• K.C. Hembram, R. Kumar, L. Kandha, P.K. Parhi, C.N. Kundu, B.K. Bindhani, Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent, Artif Cells Nanomed Biotechnol, 2018, 30001158.
• S.H. Lee, B.H. Jun, Silver Nanoparticles: Synthesis and Application for Nanomedicine. Int J Mol Sci, 2019, 3078156.
• S.M. Mousavi, S.A. Hashemi, Y. Ghasemi, A. Atapour, A.M. Amani, A. Savar Dashtaki, A. Babapoor, O. Arjmand, Green synthesis of silver nanoparticles toward bio and medical applications: review study. Artif Cells Nanomed Biotechnol, 2018, 30328732.
• L.M. Nieves, J.C. Hsu, K.C. Lau, A.D.A. Maidment, D.P. Cormode, Silver telluride nanoparticles as biocompatible and enhanced contrast agents for X-ray imaging: an in vivo breast cancer screening study, Nanoscale, 2021, 33325953.
• A.S. Jain, P.S. Pawar, A. Sarkar, V. Junnuthula, S. Dyawanapelly, Bionanofactories for Green Synthesis of Silver Nanoparticles: Toward Antimicrobial Applications. Int J Mol Sci, 2021, 34769419.
• A.S. Takamiya, D.R. Monteiro, L.F. Gorup, E.A. Silva, E.R. de Camargo, J.E. Gomes-Filho, S.H.P. de Oliveira, D.B. Barbosa, Biocompatible silver nanoparticles incorporated in acrylic resin for dental application inhibit Candida albicans biofilm, Mat Sci Eng C-Mater, 2021, 33254968.
• I.A. Wani, T. Ahmad, A. Khosla, Recent advances in anticancer and antimicrobial activity of silver nanoparticles synthesized using phytochemicals and organic polymers, Nanotechnology, 2021, 34340224.
• R.R. Miranda, I. Sampaio, V. Zucolotto, Exploring silver nanoparticles for cancer therapy and diagnosis. Colloids and Surfaces B: Biointerfaces, 2022, 34896692.
• R. Sharma, N. Srivastava, Plant Mediated Silver Nanoparticles and Mode of Action in Cancer Therapy: A Review. Anticancer Agents Medicinal Chem. 2021, 33292139.
• D. Kovács, N. Igaz, M.K. Gopisetty, M. Kiricsi, Cancer Therapy by Silver Nanoparticles: Fiction or Reality? Int J Mol Sci, 2022.
• R.B. Patil, A. D. Chougale, Analytical methods for the identification and characterization of silver nanoparticles: A brief review, Materialstoday: proceedings, 2021.
• A.C.D. Filho, J. de Jesus Soares, M.R.S. Carriço, G.P. Viçozi, W.H. Flores, C.C. Denardin, R. Roehrs, E.L.G. Denardin, Green synthesis silver nanoparticles Bougainvillea glabra Choisy/LED light with high catalytic activity in the removal of methylene blue aqueous solution. Environ Sci Pollut R, 2023, 36547835.
• M. Naveed, B. Bukhari, T. Aziz, S. Zaib, M.A. Mansoor, A.A. Khan, M. Shahzad, A.S. Dablool, M.W. Alruways, A.A. Almalki, A.S. Alamri, M. Alhomrani, Green Synthesis of Silver Nanoparticles Using the Plant Extract of Acer oblongifolium and Study of Its Antibacterial and Antiproliferative Activity via Mathematical Approaches, Molecules, 2022, 35807470.
• M. Chandhru, R. Logesh, S. Kutti Rani, N. Ahmed, N. Vasimalai, Green synthesis of silver nanoparticles from plant latex and their antibacterial and photocatalytic studies, Environ Technol, 2022, 33825663.
• N. Willian, S. Syukri, Z. Zulhadjri, H. Pardi, S. Arief, Marine plant mediated green synthesis of silver nanoparticles using mangrove Rhizophorastylosa: Effect of variable process and their antibacterial activity, F1000Research, 2022, 37359252.
• M. Arif, R. Ullah, M. Ahmad, A. Ali, Z. Ullah, M. Ali, F.A. Al-Joufi, M. Zahoor, H. Sher, Green Synthesis of Silver Nanoparticles Using Euphorbia wallichii Leaf Extract: Its Antibacterial Action against Citrus Canker Causal Agent and Antioxidant Potential, Molecules, 2022, 35684463.
• U. Habib, A. Ahmad Khan, T.U. Rahman, M.A. Zeb, W. Liaqat, Green synthesis, characterization, and antibacterial activity of silver nanoparticles using stem extract of Zanthoxylum armatum, Microsc Res Techniq, 2022, 36125078. Epub 2022 Sep 20.
• E. Dekker, P.J. Tanis, J.L.A Vleugels, P.M. Kasi, M.B .Wallace, Colorectal cancer, Lancet, 2019, 31631858.
• F. Baidoun, K. Elshiwy, Y. Elkeraie, Z. Merjaneh, G. Khoudari, M.T. Sarmini, M. Gad, M. Al-Husseini, A. Saad, Colorectal Cancer Epidemiology: Recent Trends and Impact on Outcomes, Curr Drug Targets, 2021, 33208072.
• R. Sinha, Colorectal cancer, Clin Radiol, 2021, 34579868.
• D. Penkov, V. Andonova, D. Delev, I. Kostadinov, M. Kassarova, Antioxidant Activity of Dry Birch (Betula Pendula) Leaves Extract, Folia Medica, 2018, 31188766.
• Y.A. Heo. Birch Bark Extract: A Review in Epidermolysis Bullosa, Drugs, 2023, 37658982.
• S. Hradılova, A. Panacek, R. Zborıl, Green Synthesized Silver Nanoparticles Derived From an Extract of the Betula Pendula Tree, Nanocon, Oct 17-19, Brno, 2018, Czech Republic.
• P. Devaraj, P. Kumari, C. Aarti, A. Renganathan, Synthesis and Characterization of Silver Nanoparticles Using Cannonball Leaves and Their Cytotoxic Activity against MCF-7 Cell Line, J Nanotechnol, 2013, 598328.
• S. Azizi, F. Namvar, M. Mahdavi, M.B. Ahmad, R. Mohamad, Biosynthesis of Silver Nanoparticles Using Brown Marine Macroalga, Sargassum Muticum Aqueous Extract, Materials, 2013, 28788431.
• J. Jalab, W. Abdelwahed, A. Kitaz, R. Al-Kayali, Green synthesis of silver nanoparticles using aqueous extract of Acacia cyanophylla and its antibacterial activity, Heliyon, 2021, 2405–8440.
• Z. E. Jiménez Pérez, R. Mathiyalagan, J. Markus, Y. Kim, H.M. Kang, R. Abbai, K.H. Seo, D. Wang, V. Soshnikova, D.C Yang, Ginseng-berry-mediated gold and silver nanoparticle synthesis and evaluation of their in vitro antioxidant, antimicrobial, and cytotoxicity effects on human dermal fibroblast and murine melanoma skin cell lines. Int J Nanomed, 2017, 12:709–723.
• E. F. Apriani M. Mardiyanto, A. Hendrawan, Optimization of Green Synthesis of Silver Nanoparticles From Areca Catechu L. Seed Extract With Variations of Silver Nitrate and Extract Concentrations Using Simplex Lattice Design Method, Farmacia, 2022.
• M. Bilal, T. Rasheed, H.M.N. Iqbal, C. Li, H. Hu, X. Zhang, Development of silver nanoparticles loaded chitosan-alginate constructs with biomedical potentialities. Int J Biol Macromol, 2017, 28705499.
• S. Jain, M.S. Mehata, Medicinal Plant Leaf Extract and Pure Flavonoid Mediated Green Synthesis of Silver Nanoparticles and their Enhanced Antibacterial Property, Sci Rep, 2017, 29158537.
• G, Shumi, T.B. Demissie, R. Eswaramoorthy, R.F. Bogale, G. Kenasa, T. Desalegn, Biosynthesis of Silver Nanoparticles Functionalized with Histidine and Phenylalanine Amino Acids for Potential Antioxidant and Antibacterial Activities, ACS Omega, 2023, 37457474.
• K. Anandalakshmi, J. Venugobal, V. Ramasamy, Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity, Appl Nanoscience, 2016.
• L. Salvioni, E. Galbiati, V. Collico, G. Alessio, S. Avvakumova, F. Corsi, P. Tortora, D. Prosperi, M. Colombo, Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations, Int J Nanomed, 2017, 28408822.
• A. Casas, M. Virginia Alonso, M. Oliet, E. Rojo, F. Rodríguez, FTIR analysis of lignin regenerated from Pinus radiata and Eucalyptus globulus woods dissolved in imidazolium-based ionic liquids, J Chem Technol Biot, 2012, 472–480.
• H. Yang, R. Yan, H. Chen, D. H. Lee, C. Zheng. Characteristics of hemicellulose, cellulose and lignin pyrolysis, Fuel, 2007.
• K. Raja, A. Saravanakumar, R. Vijayakumar, Efficient synthesis of silver nanoparticles from Prosopis juliflora leaf extract and its antimicrobial activity using sewage, Spectrochim Acta A, 2012, 490–494.
• P.R. Rathi Sre, M. Reka, R. Poovazhagi, M. Arul Kumar, K. Murugesan, Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam, Spectrochim Acta A, 2015, 1137–1144.
• T. G. F. Souza, V. S. T. Ciminelli, N. D. S. Mohallem, A comparison of TEM and DLS methods to characterize size distribution of ceramic nanoparticles, Journal of Physics: Conference Series, 29 November-2 December, 2016, Bento Gonçalves, Brazil.
• J. Bao, Z. Jiang, W. Ding, Y. Cao, L. Yang, J. Liu, Silver nanoparticles induce mitochondria-dependent apoptosis and late non-canonical autophagy in HT-29 colon cancer cells, Nanotechnol Rev, 2022, 1781–1788.
• N.S. Alduraihem, R.S. Bhat, S.A. Al-Zahrani, D.M. Elnagar, H.M. Alobaid, M.H. Daghestani, Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica, Processes, 2023.
• P.C. Shathviha, D. Ezhilarasan, S. Rajeshkumar, J. Selvaraj, β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells, Avicenna J Med Biotechnol, 2021, 33680372.
• P. Durai, A. Chinnasamy, B. Gajendran, M. Ramar, S. Pappu, G. Kasivelu, A. Thirunavukkarasu, Synthesis and characterization of silver nanoparticles using crystal compound of sodium para-hydroxybenzoate tetrahydrate isolated from Vitex negundo. L leaves and its apoptotic effect on human colon cancer cell lines, Eur J Med Chem, 2014, 25016231.
• M. Składanowski, P. Golinska, K. Rudnicka, H. Dahm, M. Rai, Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles, Med Microbiol Immun, 2016, 7620485.
• F.M. Aldayel, M.S. Alsobeg, A. Khalifa, In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera, Brazilian J Biol, 2022, 34346959.
• O.M. Bondarenko, M. Sihtmäe, J. Kuzmičiova, L. Ragelienė, A. Kahru, R. Daugelavičius, Plasma membrane is the target of rapid antibacterial action of silver nanoparticles in Escherichia coli and Pseudomonas aeruginosa, Int J Nanomed, 2018, 30498344.
• M. Adil, T. Khan, M. Aasim, Evaluation of the antibacterial potential of silver nanoparticles synthesized through the interaction of antibiotic and aqueous callus extract of Fagonia indica, AMB Express, 2019.
• M.A. Al Mashud, M. Moinuzzaman, M.S. Hossain, Green synthesis of silver nanoparticles using Cinnamomum tamala (Tejpata) leaf and their potential application to control multidrug resistant Pseudomonas aeruginosa isolated from hospital drainage water, Heliyon, 2022, 35855998.
• A. Panáček, L. Kvítek, M. Smékalová, Bacterial resistance to silver nanoparticles and how to overcome it, Nat Nanotechnol, 2018, 29203912.
• M. Zarei, A. Jamnejad, E. Khajehali, Antibacterial effect of silver nanoparticles against four foodborne pathogens. Jundishapur J Microb, 2014, 25147658.
• W.L.C. Bernardo, M.F.G. Boriollo, C.C. Tonon, Biosynthesis of silver nanoparticles from Syzygium cumini leaves and their potential effects on odontogenic pathogens and biofilms, Frontier Microbiol, 2022, 36246249.
• M.M.J. Arsène, P.I. Viktorovna, M. Alla, Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans. Veterinary World, 2023, 36855352.