Isocratic liquid chromatography technique for the analysis of cyanocobalamin from beef liver and heart muscle extracts

Yıl 2024, Cilt: 6 Sayı: 2, 102 - 107, 20.12.2024

Labaran Ibrahim

https://doi.org/10.51435/turkjac.1513123

Öz

Reverse-phase high-performance liquid chromatography (RP-HPLC) is among the most widely recommended techniques for analyzing water-soluble vitamins such as vitamins C and B-complexes. The research study was conducted to detect and quantify cyanocobalamin (vitamin B12) from the beef liver and heart muscle extracts using a validated isocratic RP-HPLC procedure. The analytical column was Luna® Phenomenex 5 µm C18 (2) 100 A LC-column (150 × 4.6 mm). The mobile phase consisted of water/ethanol in a ratio of 60:40 (v/v). An enzymatic digestion with 1% potassium cyanide was used for samples (beef liver and heart muscle) extraction. The validated method showed to be linear, R = 0.9977; fast, with a retention time of less than 6.00 min; precise, %RSD of 1.16 and 1.74%; and sensitive, with limits of detection and quantification of 0.00033 and 0.00100 µg/mL. The detected values of cyanocobalamin from the beef liver (BLV) and heart muscle (HRM) extracts were 52.04 ± 0.13 and 42.04 ± 0.29 µg/mL, respectively. BLV extract indicated a higher level of cyanocobalamin. Hence, the validated isocratic RP-HPLC technique can be recommended to analyze cyanocobalamin and related compounds in research laboratories such as diagnostics, foods, and pharmaceuticals.

Anahtar Kelimeler

Cyanocobalamin, ethanol, Isocratic, mobile phase, beef liver extract

Destekleyen Kurum

Rhodes University, South Africa

Teşekkür

Department gf Biochemistry and Microbiology, Rhodes University, South Africa

Isocratic liquid chromatography technique for the analysis of cyanocobalamin from beef liver and heart muscle extracts

Öz

Kaynakça

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