Investigation of antioxidant and anticholinesterase activity of Hypericum Perforatum L. extracts

Yıl 2025, Cilt: 7 Sayı: 1, 1 - 8, 31.01.2025

Demet Dincel , Yasin Darı , Çağla Kızılarslan Hançer , Nur Banu Onal , Murat Kartal , Gülaçtı Topçu

https://doi.org/10.51435/turkjac.1565559

Öz

Hypericum perforatum L. is widely known for its therapeutic properties, including wound healing, antispasmodic effects, and anxiety treatment. This study was examined the total phenolic and flavonoid content, antioxidant capacity, and anticholinesterase activity of aqueous, ethanolic, and water-ethanol extracts from the plant's aerial parts and also green chemistry and sustainability were also investigated. Extraction was performed under reflux at 60°C. The anticholinesterase effect of Hyperium perforatum L., which is known to be used in the treatment of neurological diseases such as coxalgia, paralysis, spastic paralysis, menopausal neurosis, spinal convulsion etc., was investigated in vitro [1]. Three different extracts were prepared as 100% ethanol, 50% ethanol-50% water and 100% water. The antioxidant activity of the prepared extracts was examined using Folin-Ciocalteu in terms of total phenolic-flavanoid content and then the anticholinesterase activity was investigated by Ellman method [2]. LC-HR-MS identified Rhamnocitrin as the most abundant compound in the water-ethanol extract, while Quercitrin and Hederagenin were descent in the ethanol extract. The ethanol extract demonstrated the highest total flavonoid content and enzyme inhibition rates, especially for AChE (65%) and BChE (75%). In contrast, the water extract had the highest phenolic content but lower enzyme inhibition, especially for BChE (16%). Greenness metrics analysis demonstrated superior recyclability for the aqueous extract, earning it the highest score in sustainability. In general, the ethanol extract exhibited the most potent biological activity, while the aqueous extract excelled in green chemistry principles. These findings support the potential of H. perforatum extracts for further development in natural product research.

Anahtar Kelimeler

Folin-Ciocalteu, Hypericum, Anticholinesterase Activity, Green Chemistry, LC-HR/MS

Investigation of antioxidant and anticholinesterase activity of Hypericum Perforatum L. extracts

Öz

Kaynakça

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