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While natural harvesting of truffles (Tuber sp.) has decreased worldwide in recent years, truffle cultivation has been increasing as a consequence of modern cultivation techniques. Tuber aestivum, which is known in the world as the summer truffle, is the most prevalent edible ectomycorrhizal fungus with increasing commercial interest, both in the world and in Turkey. Truffles can develop symbiotically with various forest trees and some bush species. In this study, by inoculating the seedlings of Pinus pinea (stone pine), which is a main forest species grown naturally in Turkey, with Tuber aestivum, the mycorrhizal developments that emerged were determined morphologically and it was seen that stone pine, selected as the host species, was compatible with Tuber aestivum. Ninety-two of the stone pine seeds were allowed to germinate and germination was observed in 60 seeds. The germination rate was determined to be 65%. Sixty germinated saplings were inoculated with Tuber aestivum. Mycorrhiza were observed in 46 seedlings. The success rate was recorded at 77%. Mycorrhizal dimensions were determined to be an average of 0.0023 cm.

Tuber aestivum, Pinus pinea, ectomycorrhiza, truffle cultivation, Turkey

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