Determination of mycorrhizal developments in Pinus pinea (stone pine) seedlings inoculated with Tuber aestivum (summer truffle)

Yıl 2020, Cilt: 1 Sayı: 2, 124 - 130, 01.12.2020

Sevgin Özderin

Öz

While natural harvesting of truffles (Tuber sp.) has decreased worldwide in recent years, truffle cultivation has been increasing as a consequence of modern cultivation techniques. Tuber aestivum, which is known in the world as the summer truffle, is the most prevalent edible ectomycorrhizal fungus with increasing commercial interest, both in the world and in Turkey. Truffles can develop symbiotically with various forest trees and some bush species. In this study, by inoculating the seedlings of Pinus pinea (stone pine), which is a main forest species grown naturally in Turkey, with Tuber aestivum, the mycorrhizal developments that emerged were determined morphologically and it was seen that stone pine, selected as the host species, was compatible with Tuber aestivum. Ninety-two of the stone pine seeds were allowed to germinate and germination was observed in 60 seeds. The germination rate was determined to be 65%. Sixty germinated saplings were inoculated with Tuber aestivum. Mycorrhiza were observed in 46 seedlings. The success rate was recorded at 77%. Mycorrhizal dimensions were determined to be an average of 0.0023 cm.

Anahtar Kelimeler

Tuber aestivum, Pinus pinea, ectomycorrhiza, truffle cultivation, Turkey

Proje Numarası

0

Kaynakça

• Akman, Y. (1995). Türkiye Orman Vejetasyonu. Palme Yayınevi, Ankara.
• Allı, H. & Doğan, H.H. (2019). A new genus (Balsamia) addition for Turkish mycota. Mantar Dergisi / The Journal of Fungus 10(1): 23–25. DOI: 10.30708mantar.482166.
• Alsheikh, A.M. & Trappe, J.M. (1983). Taxonomy of Phaeangium lefebvrei, a desert truffle eaten by birds. Can J Mycol 61: 1919–1925.
• Álvarez-Lafuente, A., Benito-Matías, L.F., Peñuelas-Rubira, J.L. & Suz, L.M. (2017). Multi-cropping edible truffles and sweet chestnuts: production of high-quality Castanea sativa seedlings inoculated with Tuber aestivum, its ecotype T. uncinatum, T. brumale, and T. macrosporum. Mycorrhiza 28(1): 29–38. DOI: 10.1007/s00572-017-0805-9.
• Bonito, G., Trappe, J.M. & Vilgalys, R. (2009). North American truffles in Tuberaceae: Molecular and morphological perspectives. Acta Botanica Yunnanica 31(S16):39–51.
• Boyd, C.D. & Hellebrand, K.E. (1991). Assessment of the effect of mycorrhizal fungi on drought tolerance of conifer seedlings. Can J Bot 69: 1764–1771.
• Brundrett, M., Bougher, N., Dell, B., Grove, T. & Malajczuk, N. (1996). Working with mycorrhizas in forestry and agriculture. ACIAR Monograph 32, Canberra.
• Cordell, C.E., Owen, J.H. & Marx, D.H. (1987). Mycorrhizae nursery management for improved seedling quality and field performance. Meeting the challenge of the nineties. Proceedings of the Intermountain Forest Nursery Association. Oklahoma City, Okla, GTR- RM-151, pp. 105–115.
• Davis, P.H., Mill, R.R. & Tan, K. (1988). Flora of Turkey and the East Aegean Islands (Suppl.). Vol. 10. Edinburgh University Press, Edinburgh.
• García-Montero, L.G., Di Massimo, G., García-Abril, A. & Grande, M.A. (2007). Suitability of lime trees for Tuber melanosporum culture: mycorrhizae, soil studies, and truffle harvest associated with Tilia platyphyllos and T. x vulgaris. Sydowia 59(1): 46–55.
• Güner, A., Özhatay, N., Ekim, T. & Başer, K.H.C. (2000). Flora of Turkey and the East Aegean Islands. (Suppl. 2). Vol. 11. Edinburgh University Press, Edinburgh.
• Hall, I.R., Stephenson, S.L, Buchanan, P.K., Yun, W. & Cole, A.L.J. (2003). Edible and Poisonous Mushrooms of the World. Timber Press, Portland.
• Hall, I., Brown, G.T. & Zambonelli, A. (2007). Taming the Truffle: The History, Lore and Science of the Ultimate Mushroom. Timber Press, Portland.
• Leyval, C., Turnau, K. & Haselwandter, K. (1997). Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects. Mycorrhiza 7: 139–153. DOI: 10.1007/s005720050174.
• Marozzi, G., Sánchez, S., Benucci, G.M.N., Bonito, G., Falini, L.B., Albertini, E. & Donnini, D. (2016). Mycorrhization of pecan (Carya illinoinensis) with black truffles: Tuber melanosporum and Tuber brumale. Mycorrhiza 27(3): 1–7. DOI: DOI 10.1007/s00572-016-0743-y.
• Marschner, H. (1995). Mineral Nutrition of Higher Plants. Academic Press, London, pp. 382–383.
• Marx, D.H. (1980). [Ectomycorrhizal fungus inoculation: a tool for improving forestation practices] In: Mikola, P. (Ed.) Tropical Mycorrhiza Research. Oxford University Press, New York, pp. 13–71.
• Masetti, C. & Mencuccini, M. (1991). Régenération naturelle du pin pignon (Pinus pinea L.) dans la pineta Granducale di Alberse (Parco Naturale della Maremma, Toscana, Italie). Ecologia Mediterranea 17: 103–118.
• Mirov, N.T. (1967). The Genus Pinus. The Ronald Press Company, New York, p. 602.
• Montero, G., Candela J.A., Gutiérrez, M., Pavon, J., Ortega, C., García, C.G. & Cañellas, I. (1997). Manual de Claras Para Repoblaciones de Pinus pinea L. EGMASA & Junta de Andalucia, Huelva.
• Moreno, G., Alvarado, P. & Manjón, J.L. (2014). [Hypogeous Desert Fungi] In: Kagan-Zur, V., Roth-Bejerano, N., Sitrit, Y. & Morte, A. (Eds.) Desert Truffles. Springer, Berlin, Heidelberg, pp. 3–20.
• Mousain, D., Falconnet, J., Gruez, J., Chevalier, G., Tillard, P., Bousquet, N., Plassard, C. & Cleyet-Marel, J.C. (1987). [Controlled ectomycorrhizal development of Mediterranean forest seedlings in the nursery. First results and prospects] In: Sylvia, D.M., Hung, L-L.L, Grahan, J.H. (Eds.) Proceedings of the 7th North American Conference on Mycorrhizae. University of Florida, Florida, Gainesville.
• Oria, J.A. (1989). Silvicultura y ordenación de montes productores de hongos micorrizógenos comestibles. Boletín de la Sociedad Micológica de Madrid 13: 175–188.
• Oria, J.A. (1991). Bases para la selvicultura y evaluación de montes productores de hongos micorrizógenos comestibles. Montes 26: 48–55.
• Özderin, S., Yılmaz, F. & Allı, H. (2018). Determining mycorrhiza rate in some oak species inoculated with Tuber aestivum Vittad. (summer truffle). Turkish Journal of Forestry 19(3): 226–232. DOI: 10.18182/tjf.435372.
• Özderin, S. & Allı, H. (2020). Determination of mycorrhizal developments in pecan nut seedlings inoculated with Tuber aestivum Vittad. (summer truffle). Turkish Journal of Forestry 21(2): 131–135. DOI: 10.18182/tjf.644385.
• Pruett, G.E., Bruhn, J.N. & Mihail, J.D. (2009). Greenhouse production of Burgundy truffle mycorrhizae on oak roots. New Forests 37(1): 43–52. DOI: 10.1007/s11056-008-9108-5.
• Rincón, A., Álvarez, I.F. & Joan, P. (1999). Ectomycorrhizal fungi of Pinus pinea L. in northeastern Spain. Mycorrhiza 8(5): 271–276. DOI: 10.1007/s005720050245.
• Sesli, E. & Denchev, C.M. (2014). Checklists of the myxomycetes, larger ascomycetes, and larger basidiomycetes in Turkey. 6th ed. Mycotaxon Checklists Online. http://www. mycotaxon.com/ resources/checklists/sesli-v106-checklist.pdf. [07.07.2020].
• Smith, S.E. & Read, D.J. (1997). Mycorrhizal Symbiosis. Academic Press, London.
• Stobbe, U., Büntgen, U., Sproll, L., Tegel, W., Egli, S. & Fink, S. (2012). Spatial distribution and ecological variation of re-discovered German truffle habitats. Fungal Ecology 5(5): 591–599. DOI: 10.1016/j.funeco.2012.02.001.
• Varol, Ö. (2003). Floristic composition and diversity of a Pinus pinea L. forest in the west-Anatolia Region (Mugla-Turkey). Flora Mediterranea 13: 331–346.
• Yuanzhi, T. (2016). Method for cultivating wild truffles. Google Patents (CN105349435A). https://patents.google.com/patent/CN105349435A/en [07.07.2020].
• Zambonelli, A. (1990). Confronto fra diversi metodi di conservazione dell'inoculo di Tuber spp. Micologia Italiana 19(3): 23–29.
• Zambonelli, A., Salomoni, S. & Pisi, A. (1993). Caratterizzazione anatomomorfologica delle micorrize di Tuber spp. su Quercus pubescens Willd. Micologia Italiana 22(3): 73–90.